Abstract: Objective To evaluate the biological characteristics of decellularized bovine jugular vein valved conduit treated with dyemediated photo oxidation. Methods From July 2009 to July 2010, this study was carried out in Xijing Hospital affiliated to the Fourth Military Medical University. Forty bovine jugular vein valved conduits were obtained from Qinchuan scalpers aged between 2 to 6 years with the weight ranged from 200 to 400 kg. Then, the 40 conduits were divided into four groups by random digital table with 10 in each group. The conduits were treated with glutaraldehyde in the glutaraldehyde group (GA group), were decellularized in the decellularized group (DC group), were decellularized and treated with dyemediated photo oxidation in the decellularized and dyemediated photo oxidation treated group (DP group) , and were not dealt with in the control group (CO group). Thickness, appearance, histology, water content, shrinkage temperature, breaking strength, and soluble protein level of the conduit wall and valve were detected and compared among the four groups. Results Thickness and water content of the conduit wall and valve in the DP group were similar to those in the CO group (Pgt;0.05), while thickness of both wall and valve in the DP group was less than those in the GA group (wall: 0.8±0.1 mm vs. 1.1±0.1 mm; valve: 02±0.1 mm vs. 0.3±0.1 mm, Plt;0.05), and water content of the conduit wall and valve in the DP group was more than those in the GA group (wall: 86.1%±2.2% vs. 70.4%±2.8%; valve: 87.1%±2.5% vs. 72.1%±3.1%, Plt;0.05). The breaking strength and shrinkage temperature of the DP group were similar to those of the GA group (Pgt;0.05), while the shrinkage temperature (wall: 84.7±1.4 ℃ vs. 70.4±0.3 ℃; valve: 85.7±1.5 ℃ vs. 70.7±0.6 ℃, Plt;0.05) and the breaking strength (wall: 10.4±1.1 N vs. 6.8±1.0 N; valve: 8.0±0.9 N vs. 3.2±0.6 N, Plt;0.05) of the DP group were higher than those in the CO group. Conclusion Decellularized bovine jugular vein valved conduits treated with dyemediated photo oxidation have nice biological characteristics.Key words: Dyemediated photo oxidation; Decellularized; Bovine jugular vein valved conduit; Biological characteristics
Objective To understand the status quo of medical staffs engaged in epilepsy and EEG in Shanxi Province, analyze the existing problems, and summarize the improvement and development direction of epilepsy and EEG in Shanxi Province. Methods A questionnaire survey was conducted among medical staff of epilepsy and electroencephalogram specialty in public hospitals at or above county level in whole province and municipalities. Results ① Generally speaking, there are 17 males and 473 females in this study, with an average age of 38.7 years, the youngest was 23 years-old and the oldest was 70 years-old; ② The regional distribution has a tendency of decrease from Taiyuan in Shanxi Province to the remote areas of southeast, northwest and northwest China, and the epilepsy treatment in some poverty-stricken areas have not even been carried out; ③ The shortest time of working is 3 months and the longest is more than 40 years. The proportion of junior collage students, undergraduates, masters and doctors is 24%, 50%, 25% and 1% respectivel. The professional titles of primary, medium-level, vice-senior and senior are 24%, 39%, 26% and 11% respectively. Conclusion The number of medical workers engaged in EEG specialty in Shanxi Province is insufficient, the regional development is not balanced, and the number of junior and medium-level professional titles is large. We can formulate a mobile policy to encourage experienced medical personnel to communicate with weak areas, so as to improve the overall level of epilepsy and EEG professional development in Shanxi Province.