Objective To observe the effect of autologous peripheral blood stem cells(PBSC) transplantation in the treatment of ischemic lower extremity disorders. Methods Therapeutic group:fortyfive patients received recombinant human granulocyte colony-stimulating factor 450 to 600 U/d by hypodermic injection for 5 days to mobilize stem cells.On the 6th day,PBSC were collected by COBE 6.1 Spectra Version and were injected into ischemic lower extremity. Control group:33 patients were treated with dilating vessels drugs. After operation some indexes were evaluated. Results After operation, these indexes were improved. Skin temperature and TcpO2 were improved obviously, being statistically significant difference(P＜0.05). Conclusion Autologous PBSC transplantation might be a safe and effective method for treating lower extremity ischemic disorder. It could improve the quality of life of many patients as amputation of lower extremity of foot might be avoided or reduced.