ObjectiveA competing endogenous RNA (ceRNA) regulatory network associated with long non-coding RNA (lncRNA) specific for lung adenocarcinoma (LUAD) was constructed based on bioinformatics methods, and the functional mechanism of actinfilament-associated protein 1-antisense RNA1 (AFAP1-AS1) in LUAD was analyzed, in order to provide a new direction for the study of LUAD therapeutic targets. MethodsThe gene chip of LUAD was downloaded from the Gene Expression Omnibus (GEO), and lncRNA and mRNA with differential expression between LUAD and normal tissues were screened using GEO2R online software, and their target genes were predicted by online databases to construct ceRNA networks and perform enrichment analysis. In cell experiments, AFAP1-AS1 was genetically knocked down and siRNA was constructed and transfected into LUAD cells A549 by cell transfection. CCK8, transwell, scratch assay and flow cytometry were used to detect the ability of cells to proliferate, invade, migrate and apoptosis. ResultsA total of 6 differentially expressed lncRNA and 494 differentially expressed mRNA were identified in the microarray of LUAD. The ceRNA network involved a total of 6 lncRNA, 22 miRNA, and 55 mRNA. Enrichment analysis revealed that mRNA was associated with cancer-related pathways. In cell assays, knockdown of AFAP1-AS1 inhibited cell proliferation, invasion, and migration, and AFAP1-AS1 promoted apoptosis. ConclusionIn this study, we construct a lncRNA-mediated ceRNA network, which may help to further investigate the mechanism of action of LUAD. In addition, through cellular experiments, AFAP1-AS1 is found to have potential as a therapeutic target for LUAD.
Objective To explore the Shanghai featured day surgery directory to provide policy support for further promoting the development of day surgery and focusing on appropriate diseases. Methods The data of day surgery patients discharged from Shanghai municipal hospitals within 24 hours between January 1 and September 30, 2021 or between January 1 and September 30, 2022 were collected. The number of day surgical cases, average cost and the trend of surgical diseases in 2021 and 2022 were compared. The day surgical diseases suitable for normalized epidemic prevention and control were summarized. Results A total of 35 municipal hospitals were included, including 175 201 patients. A total of 107 101 operations were performed in 2021 and 68 100 in 2022. In the second quarter of 2022, affected by the epidemic situation in Shanghai, the number of cases undergoing day surgery decreased significantly, and the average cost was not significantly affected by the epidemic situation. In 2022, 27 of the day surgery carried out in Shanghai municipal hospitals did not appear in the national recommended directory, and the number of operations for 6 diseases recovered rapidly under the normalized epidemic prevention and control. Conclusion Vigorously implementing day surgery can improve the utilization rate of hospital beds, speed up the turnover of patients, improve the utilization rate of medical resources, and reduce the hospitalization time of patients, the waiting time for surgery and the accompanying time of family members to meet the epidemic prevention requirements.