Objective To observe the ultrastructure of theca interna of the de-endothelium allogenetic blood vessels in dogs by transmission and scanning electron microscope at different phases. Methods The endothelium of the allogenetic blood vessels were first removed and cryodensiccated, and were then end to end anastomosed to canine femoral artery. Samples were collected and observed with scanning and transmission electron microscope on the first, second, fourth, eighth, twelfth, sixteenth, and twentieth week after transplantation, respectively. Results A layer of cellulose membrane was formed on the surface of allogenetic blood vessels one week after transplantation; Fusiform cells were observed at the anastomotic stoma of the allogenetic blood vessels two weeks after transplantation, and theca interna, which was covered by fusiform cells and elliptical erythrocytes, was formed twelve weeks later; Slightly hyperplastic smooth muscle cells and collagenous fibers were observed under the endothelium twelve to twenty weeks after transplantation. Conclusion The endothelium cells could cover the surface of the allogenetic blood vessels without remarkable hyperplasia of intima during a short period of time, which may suggest the satisfactory histocompatibility of canine allogenetic blood vessels.
ObjectiveTo understand the prevalence of iliac vein stenosis in crowds without lower extremityvenous disease and symptoms (abbreviated as asymptomatic crowd) and patients with lower extremity varicose vein and analyze relevant to factors of iliac vein stenosis. MethodsAccording to the inclusion and exclusion criteria, the CT imaging data and clinical informations of objects in the department of vascular surgery, the First Affiliated Hospital of Chongqing Medical University from 2020 to 2021 were collected. The objects included the patients with lower extremity varicose vein in the department of vascular surgery of this hospital and asymptomatic crowds in the physical examination center of this hospital. The occurrence of iliac vein stenosis of the objects was compared and the relevant to risk factors affecting the occurrence of iliac vein stenosis were analyzed. ResultsA total of 268 subjects who met the criteria were included in this study, the iliac vein stenosis was occurred in 63 (23.5%) subjects. there were 162 asymptomatic crowds and 106 patients with lower extremity varicose vein. The incidence of iliac vein stenosis was higher in the patients with lower extremity varicose vein than that in the asymptomatic crowds [36.8% (39/106) versus 14.8% (24/162), χ2=17.212, P<0.001]. The results of multivariate logistic regression analysis showed that the female crowds had a higher risk of iliac vein stenosis as compared with the male crowds [OR=3.131, 95%CI (1.188, 8.257), P=0.021] and the crowds with higher body mass index (BMI) had a lower risk of iliac vein stenosis [OR=0.802, 95%CI (0.666, 0.966), P=0.020] in the asymptomatic crowds, as well as the risk of iliac vein stenosis was decreased in the older patients with lower extremity varicose vein [OR=0.946, 95%CI (0.901, 0.993), P=0.026]. ConclusionFrom the results of this study, the incidence of iliac vein stenosis is not low, and the incidence rate of patient with lower extremity varicose vein is higher than that of asymptomatic crowd, and there may be associated with gender, age, or BMI.