ObjectiveTo explore the risk factors of diabetic retinopathy. MethodsWe retrospectively analyzed the clinical data of 137 patients with diabetes mellitus (DM) from July 2012 to July 2015. According to the situation of retinopathy, the patients were divided into three groups. Forty-three patients without retinopathy were regarded as the control group, 46 non-proliferative retinopathy patients as the observation group, and 48 patients with proliferative retinopathy as the trial group. DM blood pressure, blood glucose, glycosylated hemoglobin, blood lipid, albumin creatinine ratio and other indicators were collected and analyzed, and multiple-factor non-conditional logistic regression analysis was carried out. ResultsGlycosylated hemoglobin, total cholesterol, triglyceride, low density lipoproteincholesterol, high density lipoprotein cholesterol, body mass index, postprandial 2-hour blood glucose and fasting blood glucose were not significantly different among the three groups (P ＞ 0.05) , but the duration of diabetes, vascular endothelial growth factor and urinary albumin creatinine ratio were significantly different (P ＜ 0.05) . The diabetic duration, glycosylated hemoglobin, systolic blood pressure, urinary albumin creatinine ratio and vascular endothelial growth factor were independently associated with diabetic retinopathy (P ＜ 0.05) . ConclusionThe prolonged disease course of diabetic patients, unstable status of blood glucose and blood pressure, and the increase of blood vessel growth factor and vascular endothelial growth factor can cause the development of diabetic retinopathy.
ObjectiveTo assess the effect of plasmid-mediated short hairpin RNA (shRNA) on Klotho gene in mice medullary collecting duct (IMCD3) cells. MethodsThree pairs of shRNA for Klotho (the first, second, and third pairs of shRNA) were designed and pRNAU6-Klotho were constructed, which were transfected into IMCD3 cells by Lipofactine2000. The negative control group and untreated group were set up at the same time. After 24 hours, the expressions of Klotho mRNA and protein levels were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. ResultsThe second pairs of shRNA had the best interference effect compared with the control group according to RT-PCR (P<0.01). The results of Western blotting showed that the Klotho protein levels in the second pairs of shRNA group differed much from all the other 4 groups (P<0.001). ConclusionPlasma-mediated shRNA can highly inhibit the expression of Klotho, which suggests that it may be potential to study the pathogenesis in kidney disease.