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find Author "XIANG Xudong" 1 results
  • P eroxisome proliferators-activated receptor gamma-agonists inhibis the expression of phosphorylation of signal transducer and activator of transcription 6 in T-lymphocytes of patients with acute asthma in vitro

    Objective To explore the possible anti-inflammatory mechanism of peroxisome proliferators-activated receptor(PPAR) gamma-agonists by investigating the effects of Rosiglitazone on the expression of phosphorylation of signal transducer and activator of transcription 6(p-STAT6) and the secretion of interleukin(IL)-4 in T-lymphocytes from patients with acute asthma.Methods Peripheral blood T-lymphocytes from 10 healthy volunteers(group A) and 10 patients with acute asthma were isolated,purificated and cultured.T-lymphocytes from the asthma patients were divided into a control group(group B) and a Rosiglitazone treated group(group C).Rosiglitazone was added with a single dose of 10-4 mol/L at 0 hour of cultrue.After cultured for 48 hours,the concentration of IL-4 in supernatant of each groups were detected by ELISA.The express of p-STAT6 in the T-lymphocytes were determined by Western blot and immunohistochemical techniques.Results The levels of IL-4 were increased markedly in group B than those in group A and group C[(170.34±9.05)pg/mL vs(76.82±7.06)pg/mL and(123.59±8.70)pg/mL,both Plt;0.01],and which in group C was significantly lower than group A(Plt;0.01).The levels of p-STAT6 in T lymphocytes were increased markedly in group B than in group A and C[Western blot:(6.28±0.19 vs 3.07±0.18 and 4.12±0.16;immunohistochemistry:(36.58%±7.41)% vs(11.39±4.02)% and(23.92±5.8)%,all Plt;0.01),and which in group C were significantly higher than that in group B(both Plt;0.01).There was a positive correlation between the level of p-STAT6 and IL-4(Plt;0.01).Conclusion The levels of p-STAT6 and IL-4 in T-lymphocytes of patients with acute asthma were suppressed by Rosiglitazone in vitro.

    Release date:2016-09-14 11:57 Export PDF Favorites Scan
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