Objective To introduce the materials, preparative technique and endothel ial ization modification of scaffold. Methods The recent original articles about vascular tissue engineering were extensively reviewed and analyzed. Results The materials including natural materials, biodegradable polymers and composite materials were studied in the field of scaffold. The ways of casting, cell self-assembly, gel spinning and electrospinning were appl ied to prepare the scaffold of vascular tissue engineering. The modification of scaffold was one of the most important elements for vascular tissue engineering. Conclusion The recent researchs about scaffold of vascular tissue engineering focus on composite material and electrospinning, the modification of scaffold can improve the abil ity of adhesion to endothel ial cells.
To evaluate the cytocompatibil ity of Arg-Gly-Asp-recombinant spider silk protein (pNSR16) / poly vinyl alcohol (PVA) through in vitro cytotoxicity experiment and cell-material co-culture experiment. Methods pNSR16/PVA scaffold and its extraction were prepared by using solvent casting/particulate leaching method, and NIH-3T3 cells were cultivated with the extraction in vitro. The cytotoxicity of scaffold was analyzed using MTT assay 1, 3 and 5 days after culture. Scanning electron microscope and HE staining observation were conducted 2, 4 and 6 days after culturing NIH-3T3 cells on the pNSR16/PVA scaffold. Immunohistochemistry detection was performed 6 days after co-culture. Adhesion, growthand expression of the cells on the scaffold were observed. Results The cytotoxicity of pNSR16/PVA scaffold was in grade 0. Scanning electron microscope observation: the cells covered the surface of the scaffold and were arranged in a directional manner 4 days after co-culture. HE staining: the cells adhered to and grew on the surface of scaffold, and migrated into the scaffold with the increase of culture duration. Immunohistochemistry detection: bFGF was secreted by NIH-3T3 cells, and the cells differentiated normally. Conclusion pNSR16/PVA scaffold has a satisfactory cytocompatibil ity and may be an ideal tissue engineered scaffold materia