目的 探讨抗环瓜氨酸肽抗体（anti-CCP）与类风湿因子（RF）对类风湿关节炎（RA）的诊断效能，及RF分型检测在RA活动度判断中的价值。 方法 选取2012年3月－2013年2月就诊的64例RA患者为病例组，103例其他自身免疫性疾病患者为对照组，用酶联免疫吸附试验分别检测anti-CCP和RF-IgM/IgG/IgA，收集数据进行统计分析。 结果 anti-CCP与RF联合指标对RA的诊断灵敏度最高（92.2%），anti-CCP的特异度最高（95.1%）；RF-IgA的水平与骨关节侵蚀程度呈正相关（rs=0.987，P=0.000）；RF的3个亚型都可反映RA疾病的活动度（P＜0.05）。 结论 anti-CCP与RF联合诊断RA，可显著提高诊断灵敏度，RF的分型检测对于RA患者的活动度监测有重要价值。
Objective To analyze the risk factors of hospitalized children with acute asthma exacerbation in Chongqing region. Methods A total of 193 cases were randomly selected from the hospitalized children with acute asthma exacerbation in Chongqing Children’s hospital and Jiangjin District People’s Hospital from January 2009 to December 2009. A self-designed questionnaire was used to collect data. A control group of children were randomly selected from the out-patients who received regular maintain therapy without asthma attacks for more than 3 months. Results The first independent risk factor of asthma hospitalization was respiratory infection ( 85. 5%, 165 /193) . Irregular use of control medications was the second important factor for the acute exacerbation. There were 75% ( 138 /193) patients didn’t take controlmedications regularly, includes 102 undiagnosed and 36 pre-diagnosed cases which was more common than that in regular maintain therapy group ( 21/110, 19. 1% ) . A variety of allergen-induced acute exacerbation of asthma was also common, which accountted for 9. 3 % ( 18/193) . There were more boys than girls ( M/F:124 /69) and no significant difference in the family history of allergic diseases ( P gt; 0. 05) . Conclusion Respiratory infection, under-diagnosis of asthma, and irregular use of the control medications are risk factors of acute exacerbation in children with asthma in Chongqing region. Meanwhile allergen exposure warrantsmore attention.
Objective To determine the effect of methimazole (MMI) on retinal vascular development in neonatal rats, and to investigate the relationship between the concentration of insulin-like growth factor-I (IGF-I) in serum and the development of normal blood vessels and between the concentration of IGF-I and the formation of abnormal blood vessels. Methods There were 75 neonatal SpragueDawley rats in experimental group whose mothers were raised with water with 0.1% MMI at the first day of parturition. Another 50 neonatal rats were in the control group whose mothers were raised with normal water. The rats in the two groups were sub-divided into 4day and 10day subgroup, respectively. The retinal flatmount of the right eyes were stained with adenosine diphosphatase (ADPase); with the paraffin section of the left eyes, the number of nucleolus breaking through retinal inner limiting membrane was counted and the retinal blood vessels were evaluated. Serum IGF-I levels were detected by radioimmunoassay, and the weight of the neonatal rats in each group were observed and recorded. Results The incidence of retinal neovascularization in 10 day MMI group was 27%, and 0% in 4-day MMI group and control group. The serum IGF-I level in 4-day and 10-day MMI group (73.07 ng/ml, 175.13 ng/ml) was obviously lower than which in the 4-day and 10-day control group (168.73 ng/ml,306.38 ng/ml) （P=0.00）. Obvious slow growth of the neonatal rats was found in MMI group compared with which in the control group. Conculsions MMI may inhibit the normal growth of retinal blood vessels and lead neovascularization, which may relate to the initial decrease of the serum IGF-I level. (Chin J Ocul Fundus Dis, 2007, 23: 198-201)
Objective Dermal papillae cells are widely applied to reconstruction of tissue engineered hair foll icle and skin. To investigate the difference of the biological characteristics of dermal papillae cells cultured with keratinocyte medium (KM)and normal medium (NM), and to determin whether it is feasible for the reconstruction of tissue engineered hair foll icle using dermal papillae cells cultured in KM. Methods Scalp samples were obtained in rhytidectomy procedure. Dermal papillaes were isolated by two steps digestive treatment, then cultured with KM and NM in two groups. The time of dermal papillae adherence and cell outgrowth was recorded and the rate of dermal papillae adherence was determined after 5 days. As well as, the difference of cell morphology was observed through inverted phase contrast microscope. The maximum generations were determined in two groups and the cell sheets were observed by HE staining. In third-generation cells, the number of aggregates in every dish and the prol iferation by MTT were compared between two groups. Meanwhile, the expression of α-smooth muscle actin (α-SMA) and ALP were detected by immunofluorescence and specific staining in two groups. Results Dermal papillaes of KM group had a higher rate of adherence and fast outgrowth. The rates of adherence were 54.17% and 36.78% in KM group and in NM group, respectively. In KM group, cells adhered after 24 hours and outgrew after 64 hours. While, cells adhered after 48 hours and outgrew after 80 hours in NM group. The cells were bigger in NM group than in KM group. In third-generation cells, 3.06 ± 1.12 and 9.25 ± 1.73 aggregates formed in NM group and KM group, respectively, the difference was significant (P lt; 0.05). In addition, cells could form cell sheets which were muti-layers in KM group. Mostly 7 and 15 generations could been subcultured in NMgroup and KM group, respectively. The result of MTT indicated that cells prol iferated more actively in KM group; absorbance value of KM group was significantly higher than that of NM group after 7 days (P lt; 0.05). The positive of α-SMA were detected in the third-generation cells of both groups. Ocassionally a l ittle few cells expressed ALP with (987 ± 146) m2 positive area in the sixth-generation cells of NM group. However, the cells still expressed ALP with (8 757 ± 558) μm2 positive area in the fourteenthgeneration cells of KM group and the difference was significant (P lt; 0.05). Conclusion Cells proliferate actively and aggregate obviously and could been subcultured more generations in KM. Therefore, culturing dermal papillae cells with KM is feasible for the reconstruction of tissue engineered hair foll icle.