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find Author "YANG Qimei" 1 results
  • Efect of Pseudomonas aeruginosa quorum-sensing systems on airway mucus hypersecretion

    Objective To examine the effects of Pseudomonas aeruginosa(PA)quorum-sensing systems on airway mucus hypersecretion.Methods Sixty Sprague-Dawley rats were intubated with a silicone tube pre-coated with PAO1(wild-type PA strain),PAO1-JP2(quorum-sensing-mutant strain)or saline in the bronchus.After 28 days,the mRNA and protein expression of MUC5AC in the rats’bronchial epithelia were detected by RT-PCR,alcian blue/periodic acid—Schif(AB/PAS)staining and enzyme linked immunosorbent assay(ELISA).Results In the PAO1 group,bronchiolar epithelium goblet cells by AB/PAS staining was significantly more than those in the PAO1-JP2 and control groups(both Plt;0.05).The expression level of MUC5AC mRNA in the PAO1 group was significantly higher than those in the PAO1-JP2 and control groups(both P lt;0.05).The ELISA showed that the concentration of MUC5AC protein in bronchoalveolar lavage fluid(BALF)in the PAO1 group was much higher than that in the PAO1-JP2 group(P lt;0.05).Conclusion PA quorum-sensing system plays an important role in airway mucus hypersecretion

    Release date:2016-09-14 11:53 Export PDF Favorites Scan
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