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find Author "YIN Zhenhua" 1 results
  • Changes of c-kit and scf mRNA and Protein in Guinea Pig Gallbladder Fed on High Cholesterol Diet

    Objective To discuss the changes of c-kit/scf mRNA and protein in guinea pig gallbladder fed on high cholesterol diet. Methods Twenty guinea pigs were divided into two equal groups of 10 each:the control group and lithogenic group. Normal diet and high cholesterol diet was given to each group respectively. The period of stone permeation was six weeks. RT-PCR and Western blot were used to determin the expressions of c-kit and scf mRNA and protein. Results RT-PCR results showed that the expressions of c-kit mRNA(t=6.985,P<0.01) and scf mRNA (t=6.028, P<0.01)decreased significantly in lithogenic group compared with the control group. Western blot results showed that the expressions of c-kit protein (t=10.256, P<0.01) and scf protein (t=9.586, P<0.01)decreased significantly in lithogenic group compared with the control group. Conclusions The expressions of c-kit/scf mRNA and protein decrease during the formation of cholesterol gallstones in guinea pigs fed on high cholesterol diet. Inhibition of c-kit/scf pathway may play a role in the formation of cholesterol gallstones.

    Release date:2016-09-08 10:36 Export PDF Favorites Scan
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