This study is aimed to investigate the effects of mechanical stretch on the expression of transforming growth factor-β1 (TGF-β1) and fibroblast growth factor-2 (FGF-2), and the signaling pathway in human bronchial epithelioid (16HBE) cells under mechanical stretch. Using loading device with flexible substrate (FX-4000T) to stretch 16HBE cells, we found that the stretching elongation was 15%, at frequency of 1 Hz, stretching for 0.5 h, 1 h, 1.5 h and 2 h. Choosing the higher expression of TGF-β1, FGF-2 and Ca2+ group to carry out intervention experiments, we used the cells pretreated with canonical transient receptor potential 1 (TRPC1) channel antagonist SKF96365, protein kinase C (PKC) inhibitor HA-100, and thereafter mechanical stretch to interpose. Compared with those in the blank control group, TGF-β1 and FGF-2' protein and mRNA, intracellular Ca2+ fluorescence intensity were higher, and the differences were statistically significant (P < 0.05) at the 4 time points, 0.5 h, 1 h, 1.5 h and 2 h. At 0.5 h, the increasing rate was the highest. TGF-β1 protein and mRNA, FGF-2 protein and mRNA, intracellular Ca2+ luorescence intensity in the stretch+SKF96365 and stretch+HA-100 intervented group were decreased, the differences were statistically significant than those in 0.5 h stretch group (P < 0.05) without intervention. The expression of TGF-β1, FGF-2 was up-regulated in 16HBE cells under mechanical stretch, PKC, TRPC1, and Ca2+ may participate in the signal path.
ObjectiveTo investigate the prevalence and risk factors of sever early childhood caries (SECC) among 3 to 5 years old children of Uyghur and Han in Kashi city, and to provide scientific basis for the establishment of oral hygiene policies for caries prevention and diagnostic among children. MethodsAccording to the basic methods of Third National Oral Health Investigation in 2005, a stratified multistage cluster randomly sampling design was applied. The deciduous caries of 893 Uyghur and Han children aged from three to five in ten kindergartens of Kashi city were clinically examined. Data were collected by questionnaire from their parents, and the result analyzed using SPSS 17.0 software. ResultsThe caries prevalence rate of primary tooth was 74.69% and the total prevalence of SECC was 39.19%. The risk factors for SECC include lower age (OR=1.537, 95% CI 1.132 to 2.086, P=0.006), the more frequencies of drinking coke (OR=1.448, 95% CI 1.008 to 2.080, P=0.045), eating fruits (OR=1.840, 95% CI 1.347 to 2.512, P=0.000), drinking milk (OR=1.794, 95% CI 1.311 to 2.456, P=0.000), and before bedtime eating or after brush eating (OR=1.833, 95% CI 1.199 to 2.802, P=0.005), as well as the higher age of starting brush (OR=2.053, 95% CI 1.448 to 2.910, P=0.000). The protective factors for SECC include the more frequency of brushing (OR=0.612, 95% CI 0.445 to 0.844, P=0.003), and higher household income (OR=0.526, 95% CI 0.423 to 0.654, P=0.000). ConclusionThere is a high prevalence of SECC in Kashi city. It is necessary to strengthen the prevention and education activities, and perform early preventive works for caries disease of children.
Quantitative assessment of the symptoms of Parkinson's disease is the key for precise diagnosis and treatment and essential for long term management over years. The challenges of quantitative assessment on Parkinson's disease are rich information, ultra-low load, long term and large range monitoring in free-moving condition. In this paper, we developed wearable devices with multiple sensors to monitor and quantify the movement symptoms of Parkinson's disease. Five wearable sensors were used to record motion signals from bilateral forearms, legs and waist. A local area network based on low power Wi-Fi technology was built for long distance wireless data transmission. A software was developed for signal recording and analyzing. The size of each sensor was 39 mm×33 mm×16 mm and the weight was 18g. The sensors were rechargeable and able to run 12 hours. The wireless transmission radius is about 45 m. The wearable devices were tested in patients and normal subjects. The devices were reliable and accurate for movement monitoring in hospital.