This paper proposes a forced oscillation respiration resistance detector which has the characteristics of portable and friendly interface, with remote transmission function. STM32 is used to produce single frequency or complex frequency oscillation signal. In the experiments, the signal was magnified by the power amplifier to drive speaker to generate oscillates airflow into the subject's oral cavity. The analog to digital coverter of STM32 was used to measure the signals obtained by the pressure sensor and the flow sensor, and then the operation parameters were to be displayed on the TFT-LCD touch screen, and could also be transferred to the master computer. Simulated lung and volunteerism were used to verify the reliability of the detector. The test results showed that the system was reliable, and it achieved the significance in respiratory impedance detecting.
The aim of this study was to establish an assessment method for determiningα-Gal(α-1, 3-galactosyle) epitopes contained in animal tissue or animal tissue-derived biological materials with ELISA inhibition assay. Firstly, a 96 well plate was coated with Galα-1, 3-Gal/bovine serum albumin (BSA) as a solid phase antigen and meanwhile, the anti-α-Gal M86 was used to react withα-Gal antigens which contained in the test materials. Then, the residual antibodies (M86) in the supernatant of M86-Gal reaction mixture were measured using ELISA inhibition assay by theα-Gal coating plate. The inhibition curve of the ELISA inhibition assay, the R2=0.999, was well established. Checking using bothα-Gal positive materials (rat liver tissues) andα-Gal negative materials (human placenta tissues) showed a good sensitivity and specificity. Based on the presently established method, theα-Gal expression profile of rat tissues, decellular animal tissue-derived biological materials and porcine dermal before and after decellular treatment were determined. The M86 ELISA inhibition assay method, which can quantitatively determine theα-Gal antigens contained in animal tissues or animal tissue-derived biomaterials, was refined. This M86 specific antibody based-ELISA inhibition assay established in the present study has good sensitivity and specificity, and could be a useful method for determining remnantα-1, 3Gal antigens in animal tissue-derived biomaterials.