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find Author "ZHANG Jinhui." 1 results
  • Expression of Toll-Like Receptor 2 in Liver Kupffer Cell and Sinsusoidal Endothelial Cell in Hepatic Endogenous Injury in Mice

    【Abstract】Objective To observe the synthesis of TLR2 protein and its mRNA expression in Kupffer cells (KCs) and sinusoidal endothelial cells(SECs).Methods Thirty-two BALB/c mice divided into two groups (operation group and false operation group) were used to prepare the model of partial hepatic ischemia/reperfusion (I/R) injury. After injury KCs and SECs were isolated with twosteps situ perfusion technique. And these cells were dyed by rat anti-mouse TLR2 IgG and anti-rat IgG2b labeled with flurescein isothiocyanate (FITC). The sysnthesis of TLR2 protein were determined by flow cytometric (FCM) analysis and real time reverse transcription polymerase chain reaction (Real-Time RT-PCR) analysis for gene expression.Results As for KCs: TLR2 expression was significant higher in operation group, compared with false operation group 〔protein expression: (9.19±1.07)% vs (1.52±0.21)%, P<0.01; gene expression: 0.54±0.77 vs 2.62±2.19, P<0.05〕. But there were no significant differences with expression in SECs. Conclusion Synthesis of TLR2 protein and its gene expression increased in KCs in the mouse partial hepatic ischemia-reperfusion injury.

    Release date:2016-08-28 04:44 Export PDF Favorites Scan
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