ObjectivesTo evaluate the reliability and validity on quality criteria for clinical nursing practice guidelines.MethodsFive appraisers were included to assess two clinical nursing practice guidelines using the quality criteria for clinical nursing practice guidelines and AGREE Ⅱ. ICC and coefficient of consistency were adopted to assess the reliability and validity of the appraisal tools.ResultsICC for two guidelines of AIDS and infusion by the quality criteria for clinical nursing practice guidelines were 0.979 and 0.890. ICC for two guidelines of AIDS and infusion by AGREE Ⅱ were 0.957 and 0.887. The coefficient of consistency for guidelines of AIDS and infusion by the two appraisal tools were 0.880 and 0.886, both were highly consistent.ConclusionsThe quality criteria for clinical nursing practice guidelines has good reliability and validity and can be used as appraisal tool for quality evaluation of clinical nursing practice guidelines. It is strongly recommended that the users should understand the appraisal tool or should be trained to decrease the bias.