Objective To study the expression of 4 circular RNA (circRNA) in peripheral blood mononuclear cells (PBMC) of patients with epilepsy and to predict its function by bioinformatics, so as to provide basis for exploring the pathogenesis of epilepsy. Methods From May 2020 to May 2021, 22 epilepsy patients were treated in the Department of Neurology of the First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology, and 22 control group were selected. There were 13 males and 8 females in the epilepsy group, with an average age of (36.41±8.39)years. There were 11 males and 11 females in the control group, with an average age of (34.41±8.68) years. The expression levels of circRNA EFCAB2, C14orf159, PARG and TMEM39 in PBMC were detected by real-time fluorescence quantitative PCR, and their functions were predicted by bioinformatics. Results Compared with the control group, the relative expression of EFCAB2 and C14orf159 in PBMC of epileptic patients was 1.42±0.06 (t=29.41) and 1.31±0.03 (t=25.27), PARG and TMEM39 were not detected in peripheral blood PBMC. Bioinformatics analysis showed that three mirnas obtained by EFCAB2 were miR-6873-3p, miR-6739-3p and miR-7110-3p. Three mirnas were obtained by C14orf159: miR-1180-3p, miR-6501-3p, and miR-3622b-5p. The seizure-related genes were predicted by TargetScan database. EFCAB2: miR-6873-3p met the requirements of 11 downstream genes. A total of 7 downstream genes of miR-6739-3p met the requirements.A total of 14 downstream genes were eligible for miR-7110-3p and a total of 9 downstream genes were eligible for miR-6501-3p. A total of 14 downstream genes were eligible for miR-3622B-5p.miR-1180-3p has a total of 1 downstream genes that meet the requirements. Conclusions Studies have shown that two circrnas, EFCAB2 and C14orf159, may be important biological markers of epilepsy. Through bioinformatics analysis, these two circrnas may act as "molecular sponges" to regulate epilepsy. EFCAB2 has the potential to act as a "molecular sponge" for miR-6873-3p and miR-7110-3p, and it was found that miR-6873-3p and miR-7110-3 share a common downstream target gene MAP1B-which plays a role in epilepsy by regulating voltage-gated sodium channels. C14orf159 can act as a molecular sponge for miR-6501-3p to regulate the expression of CCL3 and play a role in epilepsy.
ObjectiveTo systematically review the research on pediatric treatment satisfaction of medication (TS-M). MethodsThe PubMed, Embase, Cochrane Library, CBM, WanFang Data, VIP, CNKI databases and medical scale websites were electronically searched to collect studies on pediatric TS-M from inception to November 2022. Two reviewers independently screened literature, and extracted data. Using descriptive analysis, we comprehensively reviewed the TS-M assessment tool selected for the studies of children. We evaluated the methodological quality and measurement properties of existing TS-M scales for children using the Consensus-based Standards for the selection of health Measurement Instruments (COSMIN) assessment criteria. ResultsA total of 157 studies were included, including 150 pediatric studies using TS-M evaluation tools and 7 studies on the development and validation of TS-M scales for children, covering 7 specific TS-M scales for children. Our review revealed that 67.3% of the pediatric studies used unvalidated self-administered TS-M questionnaires or interviews, 24.7% used adult TS-M scales, and only 6.0% used two pediatric-specific TS-M scales. The results of the quality assessment indicated that the development quality of existing TS-M pediatric scales was considered "doubtful" or "inadequate", and the internal consistency was "sufficient" but the structural validity was probably "uncertain". High-quality research on the content validity, test-retest reliability and construct validity of the pediatric TS-M scale was still lacking. ConclusionCurrently, the use of TS-M evaluation tools in pediatric studies has irrationalities: over 90% of pediatric studies use self-made questionnaires or adult scales to evaluate children's TS-M; and the existing pediatric TS-M scales globally have narrow applications, questionable development quality, and lack some measurement performance studies. Pediatric TS-M scales with a wide range of applications are lacking.