目的 合成可生物降解的基因载体，并分析其生物毒性及转染率。 方法 低分子量聚乙烯亚胺（PEI）通过双硫键交联合成可降解的高分子量PEI衍生物（SS-PEI），通过红外光谱和核磁波谱分析技术分析其化学结构，采用细胞活力实验和检测大鼠肝肾功能指标分析其细胞和体内毒性，并转染羟基荧光素修饰的siRNA（FAM-siRNA）分析细胞转染率。 结果 红外波谱和核磁波谱分析可见酰胺键的特征波谱，噻唑蓝法和肝肾功能指标显示SS-PEI不同剂量组与对照组的差异均无统计学意义（P＞0.05），SS-PEI/FAM-siRNA转染率为（76.0 ± 2.8）%。 结论 SS-PEI的合成可明显提高装载siRNA的效率，具有安全、高效等特点。
Granulomatous lobular mastitis (GLM) is a rare mastitis disease, which mainly occurs in childbearing period. The etiology, diagnosis and treatment of GLM are still disputed. In recent years, the role of Corynebacterium infection in the pathogenesis of GLM has attracted more and more attention. Cystic neutrophilic granulomatous mastitis (CNGM) has been proposed, and more and more studies have targeted CNGM as a unique subtype of GLM. This article summarizes the published literature on CNGM so far to discuss the research progress in clinicopathological features, microbiological findings, clinical diagnosis and treatment of CNGM.