目的:探讨人肺癌细胞转移潜能与KAI1基因表达的关系。方法:应用real-time RTPCR技术检测不同转移潜能的人肺癌细胞株和正常人成纤维细胞株MRC-5中KAI1基因mRNA的表达水平及差异。结果:人肺癌细胞株中KAI1基因mRNA表达水平均显著低于正常肺成纤维细胞株MRC-5；不同转移潜能人大细胞肺癌细胞株中KAI1基因mRNA表达水平有明显差异。结论:KAI1基因表达降低与人肺癌细胞的转移潜能高低有关！
Objective To explore the role of introducing closed-loop management in the decision execution process of hospital president’s office meeting in improving the hospital decision execution and management ability. Methods The topics of the president’s office meeting of Guang’an People’s Hospital from 2021 to 2022 were selected. The topics of the president’s office meeting were divided into 2 groups based on the introduction of closed-loop management. Among them, 2021 was used as the pre-intervention group, and 2022 was used as the post-intervention group. The completion rate of agreed topics, the rate of reconsidering deferred topics, and the impact of closed-loop management on various sequence departments of the hospital before and after intervention were observed. Results A total of 946 topics were included. Among them, there were 499 topics in the pre-intervention group, 305 topics were completed, 38 topics were deferred, and 16 topics were presented for further meetings; after intervention, there were 447 topics, 404 topics were completed, 33 topics were deferred, and 24 topics were presented for further meetings. There was a statistically significant difference in the average completion rate of agreed topics [(60.90±6.30)% vs. (89.62±7.94)%] and the average rate of reconsidering deferred topics [(40.83±18.78)% vs. (65.70±25.62)%] before and after intervention (P<0.05). The average completion rate of agreed topics in administrative, logistic and business sequence increased from (60.13±7.95) %, (67.90±22.13) % and (63.34±18.54) % to (92.41±8.25) %, (88.80±18.78) % and (84.79±18.71) %, respectively. Conclusion The introduction of closed-loop management in the decision execution process of the hospital president’s office meeting can improve the decision-making efficiency and execution ability.
【摘要】 目的 观察在表面麻醉下不作结膜瓣和传统的以穹窿为基底结膜切开的小切口白内障摘除联合5.5 mm直径人工晶状体植入术的临床效果。 方法 收集2007年1月－2010年12月资料较完整的老年性白内障160例，将160例320只眼随机分成两组，每组各80例160只眼。在表面麻醉下行小切口白内障手术。A组不做结膜瓣，行长5.5 mm角巩膜缘的平行切口； B组行以穹窿为基底的传统的结膜瓣，做长5.5 mm角巩膜平行切口；两组均行手法小切口白内障劈核摘除联合5.5 mm直径硬质人工晶状体植入。观察术后效果及术后并发症。 结果 术后1个月，患者眼部舒适者:A组154只眼（96.25%），B组141只眼（88.13%），两组差异有统计学意义（χ2=7.332，Plt;0.05）。术后结膜退缩、下垂、巨大瘢痕：A组7只眼（4.38%），B组29只眼（18.13%），两组差异有统计学意义（χ2=15.149，Plt;0.01），两组患者术后1 d、1周及1个月视力比较：1 d，A组120只眼（75.00%），B组128只眼（80.00%）（χ2=1.467，Pgt;0.05）；1周，A组130只眼（81.25%），B组132只眼（82.25%）（χ2=0.084，Pgt;0.05）；1个月，A组138只眼（86.25%），B组139只眼（86.86%）（χ2=0.027，Pgt;0.05）；角膜水肿于术后2周后均消退。 结论 在表面麻醉下不做结膜瓣较做结膜瓣小切口白内障劈核摘除术联合人工晶状体植入术省时、对眼表面破坏小，角巩膜切口较小且较为隐闭，术后恢复快。在基层医院是一种较好的白内障手术方法。【Abstract】 Objective To observe the clinical effect of non-conjunctival flaps and traditional conjunctival flaps choper extracapsular cataract extraction (ECCE) on patients with senile cataracts. Methods The clinical data of 160 patients with cataracts between January 2007 and December 2010 were collected. The patients were randomly divided into two groups with 80 patients (160 eyes) in each group. The patients in group A underwent the small-incision choper ECCE surgery with non-conjunctival flaps; while the patients in group B underwent the traditional conjunctival flaps. Then the total effective rate, incidence of complications, and satisfaction rate of the two groups were analyzed and compared. Results One month after the surgery, the comfort was found in 154 eyes (96.25%) in group A and in 141 eyes (88.13%) in group B; the difference was significant (χ2=7.332，Plt;0.05). The complications after the surgery including conjunctiva retraction, nutation, and huge scar were found in 7 eyes (4.38%) in group A and in 29 eyes (18.13%) in group B, and the difference was significant (χ2=15.149，Plt;0.01) . The visual acuity of the patients one day, one week and one month after the surgery in the two groups were: one day: 120 eyes (75.00%) in group A and 128 eyes (80.00%) in group B (χ2=1.467，Pgt;0.05); one week: 130 eyes (81.25%) in group A and 132 eyes (82.25%) in group B (χ2=0.084，Pgt;0.05); one month: 138 eyes (86.25%) in group A and 139 eyes (86.86%) in group B (χ2=0.027，Pgt;0.05). The cornea edema was alleviated half month after the surgery. Conclusion Compared with the traditional way, non-conjunctival flaps ECCE may save the surgical time, reduce the damage of the ocular surface, improve the visual acuity and received higher satisfaction rate and less conjunctive complication, which is a good surgical method in local hospital.
West China Hospital has focused on discipline construction, substantially upgrading research results by strengthening scientific research, and has made great progress in the discipline construction in recent years. This paper aims to explore common various measures for the promotion of construction of state key disciplines, so as to offer a reference for construction of state key disciplines of large-scale, general medical colleges or hospitals.
Pricing and investment of scientific and technological achievements is one of the important forms of achievement transformation. Due to its unique transformation mechanism advantages, it has become increasingly prominent in “industry-university-research” cooperative innovation, and this transformation form has gradually becomea focus of hospital achievement transformation. Based on the policies and relevant laws/regulations, from the aspects of scientific and technological achievements pricing and investment and its development history, the difference between pricing and investment and transfer/licensing, and common problems of pricing and investment practice, this paper analyzes and discusses the characteristics and the problems that may be encountered in the implementation process of pricing and investment of scientific and technological achievements. It aims to provide feasible basis and reference for the practice of pricing and investment in scientific and technological achievements in hospitals.
Hepatocellular carcinoma (HCC) is the most common liver malignancy, where HCC segmentation and prediction of the degree of pathological differentiation are two important tasks in surgical treatment and prognosis evaluation. Existing methods usually solve these two problems independently without considering the correlation of the two tasks. In this paper, we propose a multi-task learning model that aims to accomplish the segmentation task and classification task simultaneously. The model consists of a segmentation subnet and a classification subnet. A multi-scale feature fusion method is proposed in the classification subnet to improve the classification accuracy, and a boundary-aware attention is designed in the segmentation subnet to solve the problem of tumor over-segmentation. A dynamic weighted average multi-task loss is used to make the model achieve optimal performance in both tasks simultaneously. The experimental results of this method on 295 HCC patients are superior to other multi-task learning methods, with a Dice similarity coefficient (Dice) of (83.9 ± 0.88)% on the segmentation task, while the average recall is (86.08 ± 0.83)% and an F1 score is (80.05 ± 1.7)% on the classification task. The results show that the multi-task learning method proposed in this paper can perform the classification task and segmentation task well at the same time, which can provide theoretical reference for clinical diagnosis and treatment of HCC patients.
Recently, deep learning has achieved impressive results in medical image tasks. However, this method usually requires large-scale annotated data, and medical images are expensive to annotate, so it is a challenge to learn efficiently from the limited annotated data. Currently, the two commonly used methods are transfer learning and self-supervised learning. However, these two methods have been little studied in multimodal medical images, so this study proposes a contrastive learning method for multimodal medical images. The method takes images of different modalities of the same patient as positive samples, which effectively increases the number of positive samples in the training process and helps the model to fully learn the similarities and differences of lesions on images of different modalities, thus improving the model's understanding of medical images and diagnostic accuracy. The commonly used data augmentation methods are not suitable for multimodal images, so this paper proposes a domain adaptive denormalization method to transform the source domain images with the help of statistical information of the target domain. In this study, the method is validated with two different multimodal medical image classification tasks: in the microvascular infiltration recognition task, the method achieves an accuracy of (74.79 ± 0.74)% and an F1 score of (78.37 ± 1.94)%, which are improved as compared with other conventional learning methods; for the brain tumor pathology grading task, the method also achieves significant improvements. The results show that the method achieves good results on multimodal medical images and can provide a reference solution for pre-training multimodal medical images.
Abstract: Objective To evaluate the treatment efficacy of post-infarction left ventricular pseudo-aneurysm (LVPA) through surgical procedure, and explore the diagnosis and differential diagnosis details of LVPA. Methods Between May 1993 and July 2007, 7 cases were diagnosed through echocardiography aided with left ventriculography or multi-sliced computer tomography (MSCT) or magnetic resonance imaging (MRI); 6 cases with LVPA were surgically treated through different procedure that included direct closure, cut and patching or cut and sandwiching procedure choose according to its location, anatomical morphology, and comorbidity; accompanied diseases were treated by coronary artery bypass grafting(CABG) procedure. Results Six cases were diagnosed before surgery, and 1 case was diagnosed during the surgical procedure. One died from the cardiac tamponade due to rupture of LVPA before the surgical procedure, so the inhospital mortality was 14.3%（1/7）. There was no operative death. With the follow-up from 2 months to 13 years of the 6 operational survivors, 1 case died from cardiac rupture and pericardial tamponade 4 years after the repair procedure. Of the 5 surviving LVPA, the left ventricular ejection fraction(LVEF) values were from 43% to 52%, and 3 cases were in New York Heart Association (NYHA) class Ⅰ, and 2 cases were in NYHA class Ⅱ. Conclusion Echocardiography, aided with left ventriculography or MSCT or MRI, is an effective measure for diagnosis of LVPA. Surgical procedure is an effective measure to treat LVPA,but different surgical procedures, accompanied with homeochronous CABG procedure,should be adopted to deal with LVPA according its location, anatomical morphology, and accompanied deformity. The perioperative and mid-long term efficacy were good for the surgical treatment of LVPA, but it is imperative to pay attention to prevention of the recurrence and the late rupture of repaired LVPA.