ObjectiveTo detect the expression of microRNA-497(miR-497) in gastric cancer and its biological significance, and to find out its regulatory links with other genes and investigate the effect of miR-497 overexpression interference on chemotherapy sensitivity of gastric cancer cell line. MethodsNinety-four gastric cancer tissues and adjacent to cancer tissues from 2010 to 2013 in this hospital were collected. The expression of miR-497 in the gastric cancer tissue or adjacent to cancer tissue was detected by real-time PCR. SGC-7901 cell line was transfected by miR-497-negative control and miR-497-mimics, respectively. The BCL2L2 expression was measured by Western blot after upregulation of miR-497. The chemotherapy sensitivity of cell line was tested by MTT assay. Results①The expression of miR-497 was significantly downregulated in the gastric cancer tissues as compared with the adjacent to cancer tissues (P < 0.01). 2 BCL2L2 was predicted to target gene of miR-497. after miR-497 overexpression in cells, the expression of BCL2L2 protein was decreased in the miR-497-mimics cells as compared with negative control cells. The IC50 in the miR-497-mimics cells was significantly lower than that in the negative control cells(P=0.004 6). ConclusionsmiR-497 expression is frequently downregulated in gastric cancer tissue. Overexpression of miR-497 could inhibit the expression of target gene BCL2L2, and improve chemotherapy sensitivity of gastric cancer cell line, which implies that miR-497 may play an important role in carcinogenesis and therapy of gastric cancer.
ObjectiveTo evaluate the clinical manifestation, diagnosis, and treatment of intrahepatic biliary cystadenoma (IBCA). MethodsThe clinical date of 12 patients with IBCA from January 2004 to December 2013 in our hospital were retrospectively analyzed. ResultsOf the total 10 patients were female and 2 were male. The average age was 49.0 years old (16-77 49.0 years old). The course of disease were 1 month-3years (average:7 months). The clinical symptoms were right upper abdominal fullness with a sense of pain in 6 patients, right upper abdominal mass in 1 case, jaundice in 1 case, and no symptoms in 4 cases. Enhanced CT examination showed that the tumor complete capsule and there were internal septations in 11 cases, the density of internal septations was different. There was mild enhancement of cyst wall and internal septations in 9 cases. All of 12 cases received surgical resection. Only 1 case showed recurrence in 2 years after operation, the remaining 11 patients had no recurrence. ConclusionsIBCA often occurs in middle-aged women. The main clinical symptoms are abdominal fullness with a sense of pain, right upper abdominal mass or jaundice. Enhanced CT is the main method of diagnosis before operation. Radical resection of IBCA is the best treatment, and can prevent recurrence effectively.
ObjectiveTo explore the expressions of glucose regulated protein (GRP) 78 and GRP94 in rectal adenocarcinoma and their clinical significances. MethodsIn 45 paraffin-embedded sections of rectal adenocarcinoma tissues and adjacent normal tissues, the expressions of GRP78 and GRP94 were examined by EnVisionTM. ResultsThe positive rates of GRP78 and GRP94 protein expression in the rectal adenocarcinoma tissues were 53.33% (24/45) and 53.33% (24/45), while those in the adjacent normal tissues were 13.33% (6/45) and 15.56% (7/45), respectively. There was a statistical significance of the expression of GRP78 or GRP94 between the tumor tissues and the adjacent normal tissues (all P < 0.001), and it was found that the positive expression rates were relevant to the extent of differentiation, Dukes stage, and lymph node metastasis of cancer (all P < 0.05), but not to the patient's sex, age, and size of tumor (all P > 0.05). And there was a statistical significance in Spearman method about the rate of positive expression between GRP78 and GRP94 (rs=0.464, P < 0.01). ConclusionGRP78 and GRP94 protein is highly expressed in rectal adenocarcinoma tissue, related to its metastasis and invasion, might be used as a useful indicator to judge the malignant degree.