west china medical publishers
Author
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Author "Zhang Youcheng" 2 results
  • MEASURING CONDITIONS OF HUMAN HEPATIC α1-ADRENERGIC RECEPTORS WITH RADIOLIGAND BINDING ANALYSIS

    To quantitatively measure the α1-adrenergic receptors and select the suitable conditions of receptor-ligand binding assay. Radioligand binding analysis was used to measure the concentration of α1-adrenergic receptors, and the measuring conditions were selected, respectively. Under the selected conditions, the α1-adrenergic receptors in lier plasma membranes of 8 nonhepatopathy subjects were measured.Under such suitable conditions as 3H-prazosin concentration 0.1-2.0nmol/L, incubating temperature 37℃, reaction time 20 minutes and seperating free ligand from system with double layer of filter paper, the binding of 3H-prazosin to α1receptors from 8 nonhepatopathy subjects were saturable with a high affinity. The Bmax, Kd, RMC and Hill coefficient were 142.1±14.1 fmol per milligram of protein, 0.2382±0.0548 nM, 739.0±167.6 fmol per gram of liver and 0.90±0.03, respectively.The suitable conditions may be very important for measuring α1-adrenergic receptors in human liver plasma membranes, which should be considered in hepatopathy studies.

    Release date:2016-08-29 03:18 Export PDF Favorites Scan
  • EFFECTS OF NIMODIPINE ON BLOOD-PANCREATIC BARRIER PENETRANCE IN PATIENTS WITH PANCREATIC DISEASES

    To survey the penetrance of Nimodipine to blood-pancreatic barrier. Pancreatic fluid of 15 cases with pancreatic diseases were respectively sampled from pancreatic drainage tubes at different time after Nimodipine taken orally, and the concentrations of Nimodipine in the fluid were measured with high performance liquid chromatography (HPLC). The average concentration of Nimodipine in pancreatic fluid at 0.5,1,1.5,2,2.5,3,3.5,4 hours after Nimodipine administration were 4.79,7.31,9.21,8.04,6.05,5.11,3.37 and 1.19 ng/ml, respectively. The highest value of Nimodipine level in pancreatic fluid were 9.21 ng/ml at 1.5 to 2 hours following Nimodipine taken orally. These suggested that the Nimodipine can penetrate across the bloodpancreatic barrier and make its pharmacolohical effect in pancreatic tissue.

    Release date:2016-08-29 03:18 Export PDF Favorites Scan
1 pages Previous 1 Next

Format

Content