The performance of intelligent prosthetic knee has an important effect on the realization of physiological gait of transfemoral amputees. A new type of single axis hydraulic damping knee prosthesis was designed based on the analysis of physiological gait. The training methods of the stance and swing phase were proposed. Knee prosthesis test was done through simulation and measurement device. The control target of peak flexion angle during swing of knee prosthesis is chosen to be 60–70°. When the damper valve closure was 0%, maximum swing-phase knee flexion angle of knee prosthesis were (86±2)°, (91±3)° and (97±3)° with the speed of 0.8 m/s, 1.2 m/s and 1.8 m/s, respectively. Once the valve closure was changed, maximum swing-phase knee flexion angle with different speeds could be adjusted between 60° and 70° and the required valve closure percentage were separately 25%, 40% and 70%. The damping adjustment law of intelligent knee prosthesis to achieve physiological gait was revealed.