Objective To investigate the changes and significance of intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-α (TNF-α) in intestinal mucosa in rats with obstructive jaundice. MethodsTwenty-four SD rats were randomly divided into sham operation (SO) group and bile duct ligation (BDL) group, and each group were randomly divided into the day 7 and day 14 subgroup. The expression of ICAM-1 was assayed by immunohistochemistry. The level of TNF-α was determined by ELISA. The activity of myeloperoxidase (MPO) and diamine oxidase (DAO) was determined as well.ResultsOn the day 7 and 14, in the bile duct ligation group, the ICAM-1 protein was mainly expressed in the intestinal epithelia and increased with the time on (P＜0.05); the level of TNFα increased from （14.25±1.01） ng/g to （23.83±1.43） ng/g （P＜0.01）； the intestinal DAO activity decreased from （1.70±0.36） U/mg to （1.22±0.41） U/mg （P＜0.01），and plasma DAO activity increased from （6.44±1.74）U/ml to （8.93±1.29） U/ml （P＜0.01）; the MPO activity increased from （2.85±1.22 ） U/mg to （4.93±1.37） U/mg （P＜0.01）. ConclusionThe ICAM-1 expression is significantly upregulated and the level of TNFα significantly increases after bile duct ligation, which may be involved in the PMNmediated injury to intestinal mucosa.
Objective To study the role of the complement receptor 1 and 3 ( CR1 and CR3) on neutrophils in distinguishing bacterial infection in patients with acute exacerbation of chronic obstructive pulmonary disease ( AECOPD) . Methods 40 patients with AECOPD were divided into two groups according to the detection of bacteria in subairway. 20 patients with stable COPD and 20 healthy subjects with no history of smoking were also included. According to Anthonisen criteria, 40 AECOPD patients weredivided into type Ⅰ( 11 cases) , type Ⅱ ( 12 cases) , and type Ⅲ( 17 cases) . The levels of CR1 and CR3 in blood were measured by flow cytometry. Results In AECOPD patients, 25 cases were detected bacteria,and 15 cases were not detected bacteria. The level of CR1 and CR3 were highest in the bacterial infection group than other groups, and highest in type Ⅰ AECOPD patients than other types. ROC analysis showed that CR1 and CR3 had good diagnostic value in bacterial infection in AECOPD, with optimal cutoff values of 11 and 52, respectively. Conclusion CR1 and CR3 may be good index of distinguishing bacterial infection in AECOPD.
Xiyanping is used to treat infectious diseases with antibiotics in clinic. The aim of this study is to investigate the mechanism of Xiyanping through studying the effect of the combination of Xiyanping with Cefazolin on the chemotaxis and phagocytic function of peripheral blood neutrophils in mice. Ten healthy mice were in control group. Forty healthy mice in experimental group were infected with staphylococcus aureus, and were randomly divided further into four groups, i.e. model group, Xiyanping group, Cefazolin group and combination group (Xiyanping with Cefazolin). Mice in the control group and model group were given normal saline (NS) through abdomen while those in other groups were given Xiyanping, Cefazolin, and Xiyanping with Cefazolin, respectively. The chemotaxis of peripheral blood neutrophils was detected with the transwell method, and the phagocytic function of peripheral blood neutrophils was analyzed with flow cytometry (FCM). In the present study, there was no significance on the chemotactic index of peripheral blood neutrophils in all the groups (P>0.05). The actual phagocytotic rate and index of peripheral blood neutrophils in the blank group, Xiyanping group, and the combination group were significantly higher than those of the model group and Cefazolin group (P<0.05). However, those were not significant in the blank group, Xiyanping group, and the combination group (P>0.05) or between the model group and Cefazolin group (P>0.05). Our results suggested the combination of Xiyanping and Cefazolin could enhance the therapeutic effect by improving the phagocytic function of peripheral blood neutrophils.
Acute respiratory distress syndrome (ARDS) is the most common cause of acute respiratory failure. Extensive researches have been conducted for the pathophysiology of this disease, but the mortality rate remains high. Previous studies have found that catecholamines play an important role in acute lung injury, and newly discover prompted that upregulation of phagocyte-derived catecholamines augmented the acute inflammatory response in acute lung injury which provides a new way of thinking. In the current review, we describe the mechanism of the phagocyte-derived catecholamines augmenting the acute lung injury.
Objective To summarize the important role of tumor associated neutrophils (TANs) in the occurrence, development and prognosis of hepatocellular carcinoma (HCC). Methods The basic and clinical application research literatures on the correlation between TANs and the occurrence, development and prognosis of HCC were reviewed. Results As the most abundant inflammatory cells in human body, neutrophils were also an important part of the immunosuppressive microenvironment of HCC, which was closely related to the occurrence and development, immune escape and drug sensitivity of HCC. In the tumor microenvironment, the interaction between TANs, HCC cells and other components such as neutrophil extracellular traps was regulated by a variety of signal pathways. Some parameters related to neutrophils, such as neutrophils to lymphocytes ratio, had been used as effective biomarkers for predicting the clinical prognosis of HCC patients. Conclusions The exact role of neutrophils in HCC remains to be further studied. With the continuous accumulation of research results on the function of tumor associated immunosuppressive neutrophils and new therapies targeting neutrophils, more patients with HCC will benefit from immunotherapy and personalized targeted therapy.
ObjectiveTo investigate the relation between preoperative peripheral blood neutrophil/albumin (NEU/ALB) and hypersensitive C-reactive protein (hs-CRP)/ALB ratios and seroma after laparoscopic transabdominal preperitoneal (LTAPP) hernia repair. MethodsThe patients diagnosed with inguinal hernia and underwent LTAPP hernia repair surgery admitted to the Heji Hospital Affiliated to Changzhi Medical College from June 2020 to June 2023 were retrospective collected. Multivariate logistic regression analysis was conducted to identify the risk factors affecting the occurrence of seroma after LTAPP repair surgery. The area under receiver operating characteristic curve (AUC) and 95% confidence interval (95% CI) were used to evaluate the discriminatory value of preoperative peripheral blood NEU/ALB ratio and hs-CRP/ALB ratio for seroma after LTAPP repair surgery. Delong test was used to compare the discriminatory value of these indicators. Test level α=0.05. ResultsA total of 357 patients who met the inclusion criteria were included in this study, and the seroma occurred in 42 cases (11.8%). The results of multiple logistic regression analysis showed that the larger the diameter of the hernia sac [OR (95%CI)=3.021 (1.498, 6.094), P=0.002], the more intraoperative bleeding [OR (95%CI)=4.654 (2.829, 7.657), P<0.001], and the higher the NEU/ALB ratio [OR (95%CI)=2.585 (1.618, 4.130), P<0.001] and hs-CRP/ALB ratio [OR (95%CI)=1.874 (1.239, 2.834), P=0.003], the higher the probability of seroma after LTAPP hernia repair. The AUC (95%CI) of NEU/ALB and hs-CRP/ALB indicators for predicting seroma after LTAPP repair surgery were 0.750 (0.702, 0.794) and 0.762 (0.715, 0.806), respectively. The optimal cutoff values were 2.970 and 4.001, with sensitivity of 78.6% and 73.8%, and specificity of 60.3% and 65.7%, respectively. The AUC (95%CI) of the combined prediction of seroma after LTAPP repair surgery was 0.851 (0.810, 0.886), with sensitivity and specificity of 71.4% and 87.0%, respectively. The AUC of the combined prediction of seroma after LTAPP repair surgery was higher than that of ALB (Z=2.864, P=0.004) and hs-CRP/ALB alone (Z=2.956, P=0.003). ConclusionFrom the data analysis results of this study, the incidence rate of seroma after LTAPP hernia repair is not low, and the occurrence of seroma should be paid close attention to patients with large hernia sac diameter, more intraoperative bleeding, and NEU/ALB and hs-CRP/ALB ratios.