ObjectiveTo study the expression of survivin protein in primary hepatocellular carcinoma(PHC) and its relationship to the proliferation of the tumor cells and prognosis of PHC. MethodsThe expression of survivin protein and the proliferation of tumor cells marked by proliferating cell nuclear antigen (PCNA) in 48 cases of PHC were determined by immunohistochemical method. ResultsThe survivin protein was expressed in 31 of 48 cases of PHC (64.6%). The expression of PCNA was significantly higher in hepatocellular carcinoma (HCC) with positive survivin expression than in HCC with negative survivin expression. The patients with positive survivin expression had the worse prognosis than those with negative survivin expression. ConclusionThe expression of survivin may play an important role in the proliferation of PHC cells and closely associate with the prognosis of PHC, and probably become the prognostic factor and an important target of therapy.
【Abstract】ObjectiveTo study the apoptosis of gallbladder carcinoma cell line GBCSD induced by antisense oligodeoxynucleotide (ASODN) targeting survivin. MethodsASODN targeting survivin was transfected into GBCSD cells mediated by lipofectin. Cultured cells were divided into 3 groups: control group,sense oligonucleotide (SODN) group and ASODN group. After transfected for 16 h, the cultured cells were harvested and the following texts were carried out. The expression of survivin mRNA was detected by RTPCR. Flow cytometer were used to detect apoptosis. Morphological changes were observed by electron microscopy. ResultsThe expression of survivin mRNA was decreased 47.83% in ASODN group while apoptosis was increased from (0.50±0.23)% to (26.28±3.91)%. Abnormal morphological changes of cells were observed in ASODN group and apoptosis bodies were found in some gallbladder carcinoma cells. ConclusionThe expression of survivin may be decreased in GBCSD cells after ASODN transfection.ASODN targeting survivin could induce gallbladder carcinoma cells apoptosis effectively.
ObjectiveTo study the effects of sea cucumber polysaccharide (SCPS) on the proliferation and apoptosis of hepatocellular carcinoma (HCC) cells, and to explore its effect on JAK2/STAT3/survivin pathway. MethodsThe human HCC cell lines HepG2 were placed into 24-well plates after culturing to the logarithmic phase, then dealed with different concentrations (0, 50, 100, 200 μg/mL) of SCPS. The MTT assay was used to detect the effects of different concentrations of SCPS on cell proliferation at 12 h, 24 h, and 36 h. The effect of SCPS on cell apoptosis was analyzed by flow cytometry. The mRNA and protein expression levels of JAK2, STAT3, and survivin were detected by real-time quantitative PCR (qRT-PCR) and Western blot at 36 h after treatment with SCPS, respectively. Then, the human HepG2 cells treated with different concentrations (0, 50, 100, 200 μg/mL) of SCPS were subcutaneously xenografted into nude mice to observe the effect of SCPS on the growth of tumor tissues in nude mice. At the same time, the expressions of phosphorylated JAK2, STAT3, and survivin proteins in tumor tissues were detected by immunohistochemical method. ResultsAfter treatment with different concentrations of SCPS, the proliferation inhibition rate of HepG2 cells increased over time and increased SCPS concentrations (P<0.05). After 36 h cultivation time, the apoptosis rate of cells treated with different concentrations of SCPS was statistically significant (F=117.110, P<0.001) and increased with the increase of SCPS concentrations (P<0.05). The protein expression levels of JAK2, STAT3, survival and their phosphorylated proteins decreased gradually with the increase of SCPS concentrations (P<0.05), but there was no significant difference in the mRNA expression level (P>0.05). With the increase of SCPS concentration, the tumor volume of nude mice gradually reduced (P<0.05). At the same time, the results of immunohistochemical detection showed that the positive expression rates of phosphorylated JAK2, STAT3, and survivin proteins in tumor tissues decreased with the increase of SCPS concentrations (P<0.05). ConclusionFrom preliminary results of this study, SCPS could inhibit the proliferation of HCC cells and promote their apoptosis, which might be achieved by regulating the phosphorylated expressions of JAK2, STAT3, and survivin in the JAK2/STAT3/survivin pathway.
目的:通过检测髓母细胞瘤（Medulloblastoma）中凋亡抑制蛋白（survivin）的表达情况，探讨它的临床意义，为髓母细胞瘤的治疗寻求新的思路。方法:对50例髓母细胞瘤及10例正常脑组织石蜡切片行免疫组化检测，通过对survivin蛋白在髓母细胞瘤患者与正常脑组织中的表达差异，并运用相关统计分析方法分析它在不同性别、年龄段、肿瘤大小、病理类型、临床分期的表达差异及其与预后的相关性。结果:（1）髓母细胞瘤中survivin蛋白阳性表达率为72%，明显高于正常脑组织的阳性表达率0%，二者表达差异明显（Plt;0.01）；亚组分析发现在survivin蛋白阳性组（n=36）和阴性组（n=14）病例中，性别分布、肿瘤部位、肿瘤大小、病理分型等因素在两者间无明显差别（Pgt;0.05），而年龄及TM临床分期在两组间差异显著（P分别为0.044和0.027）。（2）survivin阳性患者预后与和阴性组相比差异显著（P=0.001）。结论:survivin可能参与了对髓母细胞瘤细胞凋亡的调控，并对髓母细胞瘤的发生发展可能起着重要作用。由于survivin蛋白表达与髓母细胞瘤患者年龄、临床分期及预后等也密切相关，可作为判断病情和评价预后的指标。
Objective To investigate the expressions of platelet derived growth factor (PDGF) and survivin in hepatocellular carcinoma (HCC) with portal vein tumor thrombosis (PVTT) and to evaluate the relationships among the expressions of PDGF, survivin and the proliferation of cancer cells. Methods The expression of PDGF mRNA in 16 cases in HCC with PVTT group was observed by in situ hybridization and the results were compared with that in HCC tissue group. The expressions of PDGF and survivin protein in 36 cases in HCC with PVTT group were detected with immunohistochemistry and it was found that there was a correlation between them. Flow cytometry (FCM) was used to measure the proliferation of cancer cells and it was also used to analyze the relations among PDGF, survivin and the proliferation of cancer cells. Results The expression level of PDGF mRNA in HCC with PVTT group was significantly higher than that in HCC tissue group (P＜0.01). There was a positive correlation between the expressions of PDGF and survivin protein in HCC with PVTT (P＜0.01). The degree of proliferation of cancer cells in PDGF and survivin protein positive group was significantly higher than that in negative group (P＜0.01). Conclusion PDGF and survivin gene over-expressed in HCC with PVTT group and there is a positive correlation between the expressions of PDGF and survivin protein. The proliferation of cancer cells increases as the expressions of PDGF and survivin increase.
ObjectiveTo study the role of survivin gene in the research work of tumor. MethodsLiteratures about the molecular structure, function, mechanism,distribution of survivin gene and its role in the treatment of tumor were reviewed.ResultsSurvivin was a new member of inhibitor of apoptosis protein family, expressed in almost all the human tumors independent to the expression of bcl2. The expression of survivin was significantly correlated with the poor prognosis of tumor patients. Survivin inhibited the apoptosis of tumor cells via inhibiting the activity of caspase3, the effector molecule of the apoptosis signal transduction pathway. Inhibition of the expression of survivin gene could block its effect of inhibiting apoptosis and consequently get a therapeutic effect of tumors.ConclusionSurvivin is commonly expressed in human tumor tissues. It can be identified as an important prognostic parameter of poor outcome and a new therapeutic target in cancer.
ObjectiveTo explore the expressions of survivin, p53, and Ki67 in recurrence or metastasis breast cancer tissue, and explore their correlations and clinical significance. MethodsEighty-six patients with the chest wall local recurrence, axillary or supraclavicular lymph node metastases get treated in this hospital between January 2005 and January 2010 were excised and the expressions of survivin, p53, and Ki67 were detected by immunohistochemistry test, then compared them between the recurrence and metastasis breast cancer tissues and the primary breast cancer tissues. ResultsThe positive expression rate of survivin, p53, and Ki67 in the recurrence and metastasis breast cancer tissues were significantly higher than those in the primary breast cancer tissues, survivin: 90.70% (78/86) versus 61.63% (53/86), χ2=20.014 895, Plt;0.001; p53: 68.60% (59/86) versus 52.33% (45/86), χ2=4.766 968, Plt;0.05; Ki67: 62.79% (54/86) versus 46.51% (40/86), χ2=4.597 927，Plt;0.05. The positive expression rates of survivin in the recurrence and metastasis patients with p53, Ki67 negative expression were significantly higher than those of the primary breast cancer tissue (70.37% versus 24.39%, χ2=14.071 113, Plt;0.05; 75.00% versus 39.13%, χ2=6.540 373, Plt;0.05). The correlation coefficient of survivin with p53 and Ki67 positive expressions in the recurrence and metastasis breast cancer tissue and the primary breast cancer tissue were 0.876 214, 0.773 643 and 0.725 164, 0.698 112, respectively, Plt;0.05. ConclusionThe positive expression rates of survivin, p53, and Ki67 which increase in recurrence and metastasis breast cancer tissue indicate bad prognosis.
ObjectiveTo summarize the role of survivin gene in tumor. MethodsThe research status on biological characteristics the role of survivin gene in tumor for gene therapy and clinical application was retrospectively analyzed after related domestic and foreign literatures were reviewed. ResultsSurvivin gene was by far found to be the best powerful apoptosis inhibition gene, which played antiapoptosis role mainly through inhibiting directly the activity of caspase-3 and caspase-7 in the downstream of cascade reaction. Survivin gene promoted tumor cell proliferation and differentiation through speeding up tumor cells transition of G1→S phase and eluding the recognition of tumor cells to the apoptosis in G2/M phase. Survivin gene played important role in the intermediate links of vasiformation through angiogenic factor (VEGF, bFGF, Ang-1, and COX-2). ConclusionSurvivin gene may inhibit the apoptosis, promote the proliferation and differentiation of tumor cells and tumor angiogenesis, suggested that survivin gene has potential to act as a novel tumor marker and become an indicator of malignant tumor.
Objective To investigate the inhibitory effect of survivin antisense oligonucleotides (ASODN) on proliferation of pancreatic cancer cells PANC-1. Methods The ASODN and sense oligodeoxynucleotides (SODN) were complementary to survivin sequences. FAM-marked ASODN was transfected into PANC-1 cells mediated by positive ion liposome as ASODN group. Blank control group (normal cells), negative control group (normal medium)， and SODN group were established for comparison. The transfection efficiency was detected by flow cytometry (FCM) after transfection; MTT assay was used to detect cytotoxicity; Cell morphological changes were examined by transmission electron microscopy; The cell cycle and apoptotic rate were analyzed by FCM; Immunohistochemical staining techniques were used, and the expressions of survivin were observed under light microscopy, examined and analysed by computer image. Results ①The transfection efficiency was 31.9%, 37.4%, 41.4%, 52.6%, 24.2%, 11.4%, 16.1%, and 15.5% when the transfecting concentration of ASODN was 50, 100, 150, 200, 250, 400, 600, and 800 nmol/L, respectively; The transfection efficiency was 12.0%, 50.8%, and 11.2% when the inoculated cells was 2×104/well, 2×105/well, and 2×106/well, respectively; The transfection efficiency was 58.8%, 34.0%, and 23.6% when 2 μl, 3 μl, and 4 μl liposome was used during transfection, respectively. ②Cell gap was oversize, morphous was round, adherent cells were less after transfection under fluorescence microscope. ③The inhibition rate in the ASODN group was higher than that in each control group (Plt;0.05) on 24, 36, 48 h after treating by survivin ASODN, which increased as time prolonged (Plt;0.05). ④The apoptosis showed a ladder-shaped line in the ASODN group. ⑤Apoptotic morphology was demonstrated in the ASODN group, such as apoptotic cells with nuclear chromatin highly concentrated, crescent nuclear staining aggregated by the side nuclear membrane, nucleolus disappeared by AO and EB stains. ⑥The apoptotic rate 〔（38.1±3.4）%〕 in the ASODN group was higher than that in the SODN group 〔（4.16±1.7）%〕, Plt;0.05. ⑦G2/M cell cycle arrested in the ASODN group. ⑧After transfection, the expression of survivin protein in the ASODN group was significantly lower than that of each control group (Plt;0.05). Conclusions The optimal transfection conditions are as following: the cell count of 2×105/well, concentration of ASODN 200 nmol/L, and cationic liposome oligofectamine 2 μl, respectively. Survivin ASODN can inhibit the proliferation of pancreatic cancer cells and induce their apoptosis.
【Abstract】ObjectiveTo investigate the expressions of aromatase (Arom) and survivin (Surv) in primary hepatocarcinoma(PHC), and explore their relationships with the clinicopathology of PHC. MethodsThe specimens from 47 patients with PHC were fixed in 10% formalin and routinely embedded in paraffin. The specimens were continuously sliced into 4 μmthick sections. ABC immunohistochemistry was performed to detect the expressions of Arom and Surv with polyclonal antibodies and scored them under highpower microscopy. Results The positive rates and the scores of Arom and Surv in cancer tissues were significantly higher than those of the paratumor tissues 〔Arom: 40.43% vs 21.28% (P＜0.05), 1.53±1.69 vs 0.79±1.41 (P＜0.05); Surv: 63.83% vs 31.91% (P＜0.01), 2.40±1.96 vs 1.45±1.80 (P＜0.05)〕. The score of Surv in tumors with the maximal diameter ＜5 cm (4.00±2.10) was significantly higher than that in tumors with the maximal diameter ≥5 cm (2.17±1.86)， P＜0.05. However, there was no relationship between the expressions of Arom and Surv in PHCs and other clinicopathologic features of the PHCs. The positive correlation was found between the score of Arom and that of Surv in PHCs （r=0.316，P＜0.05）. ConclusionThe expressions of Arom and Surv might be closely related to the carcinogenesis and development of PHC.