In this study we performed Tissue Doppler Imaging (TDI), two-dimensional speckle tracking imaging (2D-STI) and three-dimensional speckle tracking imaging (3D-STI) on enrolled healthy, overweight and obese groups (34 subjects in each group), respectively, to analyze cardiac structure and its function. Compared with healthy group, global longitudinal strain (GLS), global circumferential strain (GCS), global area strain(GAS) and global radial strain (GRS) decreased progressively (P＜0.05). The ratio of early diastolic mitral inflow velocity to global early diastolic strain rate of left ventricle (E/e′sr) (r=0.466, P＜0.001), GLS(r=0.502, P＜0.001), GCS(r=0.426, P＜0.001), GAS(r=0.535, P＜0.001) and GRS(r=-0.554, P＜0.001) were correlated with body mass index (BMI). E/e′sr (r=0.37, P=0.003), GLS(r=0.455, P＜0.001), GCS(r=0.282, P=0.02), GAS(r=0.412, P＜0.001) and GRS (r=-0.471, P＜0.001) were correlated with free fatty acid (FFA). Stepwise multiple linear regression revealed that BMI was independently correlated with E/e′sr, GLS, GCS, GAS and GRS. Waist to hip ratio (WHR) was independently correlated with GLS, GCS, GAS and GRS. FFA was independently correlated with E/e′sr(P＜0.05). The study showed that cardiac structure changed and impaired left ventricular global systolic and diastolic function in overweight and obes population. Moreover, BMI, WHR and FFA may be independent influence factors of cardiac function in overweight and obese population.