Shear thinning is an ideal feature of biological ink because it can reduce the chance of blocking. For extrusion based biological printing, biological ink will experience shear force when passing through the biological printer. The shear rate will increase with the increase of extrusion rate, and the apparent viscosity of shear thinning bio-ink will decrease, which makes it easier to block, thus achieving the structural fidelity of 3D printing tissue. The manufacturing of complex functional structures in tissue trachea requires the precise placement and coagulation of biological ink layer by layer, and the shear-thinning biological ink may well meet this requirement. This review focuses on the importance of mechanical properties, classification and preparation methods of shear-thinning biological ink, and lists its current application status in 3D printing tissue trachea to discuss the more possibilities and prospects of this biological material in tissue trachea.