The Effect of Different Tidal Volume Mechanical Ventilation on the Diaphragm Discharge in Rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WangYunxia , DongHui , ZhangCheng , ZhouTianyu , SunChao , WangGuangfa ,  ZhangHong

Department of Respiratory and Critical Care Medicine, First Hospital, Peking University, Beijing, 100034, China;

Corresponding author：

ZhangHong, Email: zhangh7007@hotmail.com

Keywords：

Mechanical ventilation; Diaphragm discharge; Tidal volume

DOI：

10.7507/1671-6205.2016137

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the effects of volume mechanical ventilation with different tidal on the diaphragm discharge in rats. Methods Twenty-four SD rats were randomly divided into three groups, namely a high tidal volume group, a low tidal volume group, and a control group. The rats in the high tidal volume group and the low tidal volume group underwent volume controlled ventilation with tidal volume of 10 mL/kg and 5 mL/kg, respectively. The rats in the control group breath spontaneously after anesthetization. The EMGdi frequency, diaphragm discharge area, product of diaphragm discharge amplitude and diaphragm discharge rate (A×R) were measured every 2 hours to analyze the characteristics of diaphragm of rats under different duration of ventilation. Results Compared with the control group, there was no statistical difference of A×R in the high tidal volume group, but the frequency of the diaphragm discharge reduced and the discharge diaphragm area increased. When compared the low tidal volume group with the control group, only the A×R increased significantly. The transcutaneous oxygen saturation (SpO₂) and end-tidal CO₂ pressure (PetCO₂) in the high tidal volume group decreased significantly compared to the control group while the other indexes had no difference. Conclusions The effects of mechanical ventilation with different tidal volume on the rat diaphragm discharge are different. The low tidal volume mechanical ventilation can excite the respiratory center and strengthen the diaphragm discharge with the stabilization of physiological index while the high tide volume inhibits diaphragm function and damages the oxygenation.

Citation： WangYunxia, DongHui, ZhangCheng, ZhouTianyu, SunChao, WangGuangfa, ZhangHong. The Effect of Different Tidal Volume Mechanical Ventilation on the Diaphragm Discharge in Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(6): 602-606. doi: 10.7507/1671-6205.2016137 Copy

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3.	Wilson TA, Legrand A, Gevenois PA, et al. Respiratory effects of the external and internal intercostal muscles in humans. J Physiol, 2001, 530(Pt 2):319-330.
4.	Smith IJ, Godinez GL, Singh BK, et al. Inhibition of Janus kinase signaling during controlled mechanical ventilation prevents ventilation-induced diaphragm dysfunction. FASEB J, 2014, 28:2790-2803.
5.	Charles MP, Kali A, Easow JM, et al. Ventilator-associated pneumonia. Australas Med J, 2014, 31:334-344.
6.	Roan E, Waters CM. What do we know about mechanical strain in lung alveoli? Am J Physiol Lung Cell Mol Physiol, 2011, 301:L625-L635.
7.	Ricard JD, Dreyfuss D, Saumon G. Ventilator-induced lung injury. Eur Respir J Suppl, 2003, 42:2s-9s.
8.	Supinski GS, Callahan LA. Diaphragm weakness in mechanically ventilated critically ill patients. Crit Care, 2013, 17:R120.
9.	McCool FD, Tzelepis GE. Dysfunction of the diaphragm. N Engl J Med, 2012, 366:932-942. Erratum in:N Engl J Med, 2012, 366:2138.
10.	Vassilakopoulos T, Petrof BJ. Ventilator-induced diaphragmatic dysfunction. Am J Respir Crit Care Med, 2004, 169:336-341.
11.	Jaber S, Petrof BJ, Jung B, et al. Rapidly progressive diaphragmatic weakness and injury during mechanical ventilation in humans. Am J Respir Crit Care Med, 2011, 183:364-371.
12.	Shanely RA, Zergeroglu MA, Lennon SL, et al. Mechanical ventilation-induced diaphragmatic atrophy is associated with oxidative injury and increased proteolytic activity. Am J Respir Crit Care Med, 2002, 166:1369-1374.
13.	Kiwull-Schöne H, Wiemann M, Frede S, et al. A novel inhibitor of the Na+/H+ exchanger type 3 activates the central respiratory CO₂ response and lowers the apneic threshold. Am J Respir Crit Care Med, 2001, 164:1303-1311.
14.	Luo YM, Wu HD, Tang J, et al. Neural respiratory drive during apnoeic events in obstructive sleep apnoea. Eur Respir J, 2008, 31:650-657.
15.	Dreyfuss D, Soler P, Basset G, et al. High inflation pressure pulmonary edema:respective effects of high airway pressure, high tidal volume, and positive end-expiratory pressure. Am Rev Respir Dis, 1988, 137:1159-1164.
16.	Plataki M, Hubmayr RD. The physical basis of ventilator-induced lung injury. Expert Rev Respir Med, 2010, 4:373-385.
17.	Sassoon CS, Caiozzo VJ, Manka A, et al. Altered diaphragm contractile properties with controlled mechanical ventilation. J Appl Physiol (1985), 2002, 92:2585-2595.
18.	Beck J, Gottfried SB, Navalesi P, et al. Electrical activity of the diaphragm during pressure support ventilation in acute respiratory failure. Am J Respir Crit Care Med, 2001, 164:419-424.

1. Marini JJ. Mechanical ventilation:past lessons and the near future.Crit Care, 2013, 17(Suppl 1):S1.
2. Tremblay LN, Slutsky AS. Ventilator-induced lung injury:from the bench to the bedside. Intensive Care Med, 2006, 32:24-33.
3. Wilson TA, Legrand A, Gevenois PA, et al. Respiratory effects of the external and internal intercostal muscles in humans. J Physiol, 2001, 530(Pt 2):319-330.
4. Smith IJ, Godinez GL, Singh BK, et al. Inhibition of Janus kinase signaling during controlled mechanical ventilation prevents ventilation-induced diaphragm dysfunction. FASEB J, 2014, 28:2790-2803.
5. Charles MP, Kali A, Easow JM, et al. Ventilator-associated pneumonia. Australas Med J, 2014, 31:334-344.
6. Roan E, Waters CM. What do we know about mechanical strain in lung alveoli? Am J Physiol Lung Cell Mol Physiol, 2011, 301:L625-L635.
7. Ricard JD, Dreyfuss D, Saumon G. Ventilator-induced lung injury. Eur Respir J Suppl, 2003, 42:2s-9s.
8. Supinski GS, Callahan LA. Diaphragm weakness in mechanically ventilated critically ill patients. Crit Care, 2013, 17:R120.
9. McCool FD, Tzelepis GE. Dysfunction of the diaphragm. N Engl J Med, 2012, 366:932-942. Erratum in:N Engl J Med, 2012, 366:2138.
10. Vassilakopoulos T, Petrof BJ. Ventilator-induced diaphragmatic dysfunction. Am J Respir Crit Care Med, 2004, 169:336-341.
11. Jaber S, Petrof BJ, Jung B, et al. Rapidly progressive diaphragmatic weakness and injury during mechanical ventilation in humans. Am J Respir Crit Care Med, 2011, 183:364-371.
12. Shanely RA, Zergeroglu MA, Lennon SL, et al. Mechanical ventilation-induced diaphragmatic atrophy is associated with oxidative injury and increased proteolytic activity. Am J Respir Crit Care Med, 2002, 166:1369-1374.
13. Kiwull-Schöne H, Wiemann M, Frede S, et al. A novel inhibitor of the Na+/H+ exchanger type 3 activates the central respiratory CO₂ response and lowers the apneic threshold. Am J Respir Crit Care Med, 2001, 164:1303-1311.
14. Luo YM, Wu HD, Tang J, et al. Neural respiratory drive during apnoeic events in obstructive sleep apnoea. Eur Respir J, 2008, 31:650-657.
15. Dreyfuss D, Soler P, Basset G, et al. High inflation pressure pulmonary edema:respective effects of high airway pressure, high tidal volume, and positive end-expiratory pressure. Am Rev Respir Dis, 1988, 137:1159-1164.
16. Plataki M, Hubmayr RD. The physical basis of ventilator-induced lung injury. Expert Rev Respir Med, 2010, 4:373-385.
17. Sassoon CS, Caiozzo VJ, Manka A, et al. Altered diaphragm contractile properties with controlled mechanical ventilation. J Appl Physiol (1985), 2002, 92:2585-2595.
18. Beck J, Gottfried SB, Navalesi P, et al. Electrical activity of the diaphragm during pressure support ventilation in acute respiratory failure. Am J Respir Crit Care Med, 2001, 164:419-424.

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Chinese Journal of Respiratory and Critical Care Medicine

The Effect of Different Tidal Volume Mechanical Ventilation on the Diaphragm Discharge in Rats

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