Effects of transthyretin on biological behavior of retinal microvascular epithelial cell_Chinese Journal of Ocular Fundus Diseases

Authors：

ZhuangMiao , ShaoJun , TanChengye ,  姚勇

Department of Ophthalmology, Wuxi People's Hospital, Wuxi 214023, China;

Corresponding author：

姚勇, Email: pard1@126.com

Keywords：

Prealbumin/agonists; Retinal vessels/cytology; Endothelial cells/physiology

DOI：

10.3760/cma.j.issn.1005-1015.2015.04.014

Video：

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Objective To explore the effects of transthyretin (TTR) on biological behavior of retinal microvascular epithelial cell (RMVEC). Methods RMVEC was cultured in medium with 0 μmol/L and 4 μmol/L TTR. The proliferation, migration and healing abilities (0, 24, 48 hours) of RMVEC with different concentrations of TTR were measured by methyl thiazol tetrazolium (MTT) assay, transwell assay and scarification test. Results MTT assay shows that RMVEC with the concentrations of 4 μmol/L TTR [absorbance (A) value=0.17±0.02] glows faster than with the concentrations of 0 μmol/L TTR (A value=0.40±0.03), the difference was statistically significant (t=15.47, P=0.000 1). The transwell assay shows RMVEC with the concentration of 4 μmol/L TTR [(140±7) cells] migrants faster than RMVEC with the concentration of 0 μmol/L TTR [(227±14) cells], the difference was statistically significant (t=5.44, P=0.000 6). The scarification test shows that the RMVEC with the concentration of 4 μmol/L TTR [(134.4±45.4) μm] heals faster than the RMVEC with the concentration of 0 μmol/L TTR [(330.0±23.1) μm], the difference was statistically significant (t=8.25, P<0.01). The cells in 48 hours and 4 μmol/L group were healed completely, but not healed in 0 μmol/L group. Conclusion TTR can promote the proliferation, migration and healing abilities of RMVEC.

Citation： ZhuangMiao, ShaoJun, TanChengye. Effects of transthyretin on biological behavior of retinal microvascular epithelial cell. Chinese Journal of Ocular Fundus Diseases, 2015, 31(4): 368-370. doi: 10.3760/cma.j.issn.1005-1015.2015.04.014 Copy

1.	Cavallaro T, Martone RL, Dwork AJ, et al. The retinal pigment epithelium is the unique site of transthyretin synthesis in the rat eye[J]. Invest Ophthalmol Vis Sci, 1990, 31(3): 497-501.
2.	Zou X, Dong F, Zhang S, et al. Transthyretin Ala36Pro mutation in a Chinese pedigree of familial transthyretin amyloidosis with elevated vitreous and serum vascular endothelial growth factor[J]. Exp Eye Res, 2013, 110: 44-49.
3.	谢渊,赵艳,周建奖,等.一个遗传性玻璃体淀粉样变性家系TTR基因的突变检测[J].中华医学遗传学杂志,2012,29(1):13-15.
4.	Shi Y, Li J, Hu J, et al. A new Arg54Gly transthyretin gene mutation associated with vitreous amyloidosis in Chinese[J]. Eye Sci, 2011, 26(4): 230-238.
5.	邵珺,辛瑜,李荣秀,等.病理性近视眼患者血清蛋白质组学分子标志物筛选[J].中华眼科杂志,2012,48(3):246-252.
6.	邵珺,辛瑜,樊莹,等.转甲状腺素蛋白作为高度近视血清潜在分子标记物的研究[J].中华眼底病杂志,2011,27(3):255-258.
7.	蒋伏松,成兴波,张慧.血清视黄醇结合蛋白4在2型糖尿病视网膜病中的价值[J].中华糖尿病杂志,2010,2(5):339-343.
8.	Hernández C, García-ramírez M, Colomé N, et al. Identification of new pathogenic candidates for diabetic macular edema using fluorescence-based difference gel electrophoresis analysis[J].Diabetes Metab Res Rev,2013, 29(6): 499-506.
9.	Shao J, Xin Y, Li R, et al. Vitreous and serum levels of transthyretin (TTR) in high myopia patients are correlated with ocular pathologies[J]. Clin Biochem, 2011, 44(8-9): 681-685.
10.	陈国海,李涛,郑钦象,等.补体C4b及甲状腺素转运蛋白在增生性玻璃体视网膜病变中的表达变化及意义[J].中华眼科杂志,2011,47(8):726-731.
11.	李涛,马红婕,梁小玲,等.重组AAV2-色素上皮衍生因子对人视网膜微血管内皮细胞增殖的影响[J].中华眼科杂志,2011,47(3):197-201.
12.	胡夏云,贺涛,邢怡桥,等.15羟二十四碳四烯酸对缺氧视网膜微血管内皮细胞增生的抑制作用及其机制[J].中华实验眼科杂志,2014,32(12):1067-1073.
13.	Ong DE, Davis JT, O'Day WT,et al.Synthesis and secretion of retinol-binding protein and transthyretin by cultured retinal pigment epithelium[J]. Biochemistry, 1994,33(7): 1835-1842.
14.	白晓苏,龚莉琳,张素华,等.不同血压水平与转甲状腺素蛋白的关系研究[J].江西医学院学报,2008,48(3):40-42,48.
15.	白晓苏,龚莉琳,张素华,等.肥胖患者转甲状腺素蛋白水平的研究[J].第三军医大学学报,2007,29(10):970-972.
16.	Van Aken E, De Letter EA, Veckeneer M, et al. Transthyretin levels in the vitreous correlate with change in visual acuity after vitrectomy[J]. Br J Ophthalmol, 2009, 93(11): 1539-1545.
17.	Mullins RF, Russell SR, Anderson DH, et al. Drusen associated with aging and age-related macular degeneration contain proteins common to extracellular deposits associated with atherosclerosis, elastosis, amyloidosis, and dense deposit disease[J]. FASEB J, 2000, 14(7): 835-846.
18.	郭悦江,王道虎,陈旭,等.小干扰RNA沉默转甲状腺素蛋白基因表达对前列腺癌PC3细胞株生物学行为的影响及其机制[J].中华实验外科杂志,2012,29(12):2360-2363.
19.	Nunes RJ, De Oliveira P, Lages A, et al. Transthyretin proteins regulate angiogenesis by conferring different molecular identities to endothelial cells[J]. J Biol Chem, 2013, 288(44): 31752-31760.
20.	Chatterji B, Borlak J. A 2-DE MALDI-TOF study to identify disease regulated serum proteins in lung cancer of c-myc transgenic mice[J]. Proteomics, 2009, 9(4): 1044-1056.
21.	Ritorto MS, Borlak J. Combined serum and tissue proteomic study applied to a c-Myc transgenic mouse model of hepatocellular carcinoma identified novel disease regulated proteins suitable for diagnosis and therapeutic intervention strategies[J].J Proteome Res,2011, 10: 3012-3030.

1. Cavallaro T, Martone RL, Dwork AJ, et al. The retinal pigment epithelium is the unique site of transthyretin synthesis in the rat eye[J]. Invest Ophthalmol Vis Sci, 1990, 31(3): 497-501.
2. Zou X, Dong F, Zhang S, et al. Transthyretin Ala36Pro mutation in a Chinese pedigree of familial transthyretin amyloidosis with elevated vitreous and serum vascular endothelial growth factor[J]. Exp Eye Res, 2013, 110: 44-49.
3. 谢渊,赵艳,周建奖,等.一个遗传性玻璃体淀粉样变性家系TTR基因的突变检测[J].中华医学遗传学杂志,2012,29(1):13-15.
4. Shi Y, Li J, Hu J, et al. A new Arg54Gly transthyretin gene mutation associated with vitreous amyloidosis in Chinese[J]. Eye Sci, 2011, 26(4): 230-238.
5. 邵珺,辛瑜,李荣秀,等.病理性近视眼患者血清蛋白质组学分子标志物筛选[J].中华眼科杂志,2012,48(3):246-252.
6. 邵珺,辛瑜,樊莹,等.转甲状腺素蛋白作为高度近视血清潜在分子标记物的研究[J].中华眼底病杂志,2011,27(3):255-258.
7. 蒋伏松,成兴波,张慧.血清视黄醇结合蛋白4在2型糖尿病视网膜病中的价值[J].中华糖尿病杂志,2010,2(5):339-343.
8. Hernández C, García-ramírez M, Colomé N, et al. Identification of new pathogenic candidates for diabetic macular edema using fluorescence-based difference gel electrophoresis analysis[J].Diabetes Metab Res Rev,2013, 29(6): 499-506.
9. Shao J, Xin Y, Li R, et al. Vitreous and serum levels of transthyretin (TTR) in high myopia patients are correlated with ocular pathologies[J]. Clin Biochem, 2011, 44(8-9): 681-685.
10. 陈国海,李涛,郑钦象,等.补体C4b及甲状腺素转运蛋白在增生性玻璃体视网膜病变中的表达变化及意义[J].中华眼科杂志,2011,47(8):726-731.
11. 李涛,马红婕,梁小玲,等.重组AAV2-色素上皮衍生因子对人视网膜微血管内皮细胞增殖的影响[J].中华眼科杂志,2011,47(3):197-201.
12. 胡夏云,贺涛,邢怡桥,等.15羟二十四碳四烯酸对缺氧视网膜微血管内皮细胞增生的抑制作用及其机制[J].中华实验眼科杂志,2014,32(12):1067-1073.
13. Ong DE, Davis JT, O'Day WT,et al.Synthesis and secretion of retinol-binding protein and transthyretin by cultured retinal pigment epithelium[J]. Biochemistry, 1994,33(7): 1835-1842.
14. 白晓苏,龚莉琳,张素华,等.不同血压水平与转甲状腺素蛋白的关系研究[J].江西医学院学报,2008,48(3):40-42,48.
15. 白晓苏,龚莉琳,张素华,等.肥胖患者转甲状腺素蛋白水平的研究[J].第三军医大学学报,2007,29(10):970-972.
16. Van Aken E, De Letter EA, Veckeneer M, et al. Transthyretin levels in the vitreous correlate with change in visual acuity after vitrectomy[J]. Br J Ophthalmol, 2009, 93(11): 1539-1545.
17. Mullins RF, Russell SR, Anderson DH, et al. Drusen associated with aging and age-related macular degeneration contain proteins common to extracellular deposits associated with atherosclerosis, elastosis, amyloidosis, and dense deposit disease[J]. FASEB J, 2000, 14(7): 835-846.
18. 郭悦江,王道虎,陈旭,等.小干扰RNA沉默转甲状腺素蛋白基因表达对前列腺癌PC3细胞株生物学行为的影响及其机制[J].中华实验外科杂志,2012,29(12):2360-2363.
19. Nunes RJ, De Oliveira P, Lages A, et al. Transthyretin proteins regulate angiogenesis by conferring different molecular identities to endothelial cells[J]. J Biol Chem, 2013, 288(44): 31752-31760.
20. Chatterji B, Borlak J. A 2-DE MALDI-TOF study to identify disease regulated serum proteins in lung cancer of c-myc transgenic mice[J]. Proteomics, 2009, 9(4): 1044-1056.
21. Ritorto MS, Borlak J. Combined serum and tissue proteomic study applied to a c-Myc transgenic mouse model of hepatocellular carcinoma identified novel disease regulated proteins suitable for diagnosis and therapeutic intervention strategies[J].J Proteome Res,2011, 10: 3012-3030.

Chinese Journal of Ocular Fundus Diseases

Effects of transthyretin on biological behavior of retinal microvascular epithelial cell

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