The effects of the bone marrow mesenchymal stem cells on the expression of neurotrophic factor protein gene in rabbits with retinal detachment_Chinese Journal of Ocular Fundus Diseases

Authors：

FengChengcheng , DengAijun , SunYan ,  LiuFei

Weifang Medical University, Weifang 261053, China (Feng Chengcheng); Department of Ophthalmology, Weifang Medical University Affiliated Hospital, Weifang 261031 (Deng Aijun, Sun Yan); Department of Ophthalmology, No. 89 Hospital of PLA, Weifang 261023 (Liu Fei, now at Department of Ophthalmology, The Second Sanatorium of Qingdao of Jinan Military Region, Weifang 261023, China);

Corresponding author：

LiuFei, Email: 1245322571@qq.com

Keywords：

Retinal detachment/therapy; Mesenchymal stem cell transplantation; Nerve growth factors; Animal experimentation

DOI：

10.3760/cma.j.issn.1005-1015.2016.02.016

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Objective To observe the effects of the bone marrow mesenchymal stem cells (BMSCs) on the expression of neurotrophic factor protein gene in the retinal detachment (RD) rabbits. Methods 60 healthy rabbits were randomly divided into control group (group A), retinal detachment with PBS group (group B), retinal detachment with BMSCs group (group C), 20 rabbits in each group. RD model were established for rabbits in group B and C. 10 μl PBS was injected into the subretinal space of rabbits in group B, while 10 μl CM-Dil labeled BMSC PBS was injected into subretinal space of rabbits in group C. The rabbits in the group A received no treatment. At 1, 2 and 4 weeks after modeling, the mRNA expression of basic fibroblast growth factor (bFGF), brain derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) were measured by real-time quantitative PCR. Results At 1, 2 and 4 weeks after modeling, the mRNA expression of bFGF, BDNF, CNTF on retinal tissue were increased significantly in group C as compared with group A and B (P < 0.01). At 1 week after modeling, the mRNA expression of bFGF and CNTF on retinal tissue were increased significantly in group B as compared with group A, the mRNA expression of BDNF on retinal tissue in group B was similar with group C. At 2 and 4 weeks after modeling, the mRNA expression of bFGF, BDNF, CNTF were decreased in group B as compared with group A. Conclusion Subretinal transplantation of BMSC can increase the mRNA expression of bFGF, BDNF and CNTF on retinal tissue in RD rabbits.

Citation： FengChengcheng, DengAijun, SunYan, LiuFei. The effects of the bone marrow mesenchymal stem cells on the expression of neurotrophic factor protein gene in rabbits with retinal detachment. Chinese Journal of Ocular Fundus Diseases, 2016, 32(2): 184-186. doi: 10.3760/cma.j.issn.1005-1015.2016.02.016 Copy

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1. Golubovic M. Rhegmatogenous retinal detachment and conventional surgical treatment [J]. Prilozi, 2013,34(1):161-166.
2. Amy CY, Tiffany TT, Raymond LM,et al. Apoptosis and other cell death mechanisms after retinal detachment: implications for photoreceptor rescue[J]. Ophthalmologica,2011,226(1): 10-17.DOI: 10.1159/000328206.
3. Thaddeus S, Miyoshi H. The role of stromal stem cells in tissue regeneration and wound repair[J].Science,2009,324(5935):1666-1669. DOI: 10.1126/science. 1172687.
4. Cédric Ménard, Karin Tarte. Immunoregulatory properties of clinical grade mesenchymal stromal cells: evidence, uncertainties, and clinical application[J]. Stem Cell Res Ther,2013,4(3):64.DOI:10.1186/scrt214.
5. Skaper SD. The neurotrophin family of neurotrophic factors: an overview[J]. Methods Mol Biol,2012,846:1-12.DOI: 10.1007/978-1-61779-536-7_1.
6. Bessero AC, Clarke PG. Neuroprotection for optic nerve disorders[J]. Curr Opin Neurol,2010,23(1):10-15.DOI: 10.1097/WCO.0b013e3283344461.
7. Levkovitch-Verbin H, Sadan O, Vander S, et al. Intravitreal injections of neurotrophic factors secreting mesenchymal stem cells are neuroprotective in rat eyes following optic nerve transection[J]. Invest Ophthalmol Vis Sci, 2010,51(12):6394-6400. DOI:10.1167/iovs.09-4310.
8. 朱长庚.神经解剖学[M].北京:人民卫生出版社,2002:375.Zhu CG. Neuroanatomy[M].Beijing: People's Medical Publishing House,2002:375.

Chinese Journal of Ocular Fundus Diseases

The effects of the bone marrow mesenchymal stem cells on the expression of neurotrophic factor protein gene in rabbits with retinal detachment

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