Relationship between macular pigment optical density and serum concentration of lutein and zeaxanthin in an adult population_Chinese Journal of Ocular Fundus Diseases

Authors：

YuJie , ZhouHaiying , ZhaoMeng , CuiLei ,  LiuNingpu

Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing 100730, China(Cui Lei, now working at the Department of ophthalmology, Yuhuangding Hospital Affiliated to Qingdao University, Yantai 264000, China);

Corresponding author：

LiuNingpu, Email: nliu001@sekwa.org

Keywords：

Lutein; Carotenoids; Macular degeneration/prevention and control

DOI：

10.3760/cma.j.issn.1005-1015.2016.05.014

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To study the relationship between macular pigment optical density (MPOD) and serum concentration of lutein and zeaxanthin in an adult population. Methods Twenty patients with mild cataract and 39 healthy subjects were enrolled in this study, including 15 males and 44 females. The average age was 43.75 years. Fifty-three subjects were non-smokers and 6 male subjects were smokers. Two subjects preferred meat diet, 22 preferred meat-less diet, and 35 have balanced diet. MPOD was measured using heterochromatic flicker photometry at 0.25, 0.5, 1.0 and 1.75 degrees, and serum concentration of lutein and zeaxanthin was measured using high-performance liquid chromatography. The relationship between MPOD and serum concentration of lutein and zeaxanthin was analyzed. The differences of serum lutein and zeaxanthin between different gender, smokers and non-smokers and subjects with different dietary pattern were also analyzed. Results MPOD at 0.25, 0.5, 1.0 and 1.75 degrees were 0.59, 0.48, 0.34 and 0.18, and the average concentration of lutein and zeaxanthin were (0.45±0.16) μmol/L and (0.11±0.04) μmol/L respectively. Serum concentration of lutein and zeaxanthin in males were slightly higher than that in females, but it was not statistically significant (t=1.13, 0.86; P=0.27, 0.40). The differences of serum lutien and zeaxanthin between smokers and non-smokers (t=-0.15, -0.11; P=0.87, 0.91), among subjects of 3 dietary patterns groups were not statistically significant (F_lutein=3.87, 4.05, 0.18; P=0.83, 0.81, 0.99. F_zeaxanthin＝0.99, 1.51, 0.52; P＝0.85, 0.68, 0.72). There was no correlation between MPOD and serum concentration of lutein (r=-0.06,-0.02,-0.07,0.03;P>0.05)and zeaxanthin(r=0.02,0.12,0.09,0.11;P>0.05). Conclusion MPOD was not statistically significantly correlated with serum concentration of lutein and zeaxanthin in the studied population.

Citation： YuJie, ZhouHaiying, ZhaoMeng, CuiLei, LiuNingpu. Relationship between macular pigment optical density and serum concentration of lutein and zeaxanthin in an adult population. Chinese Journal of Ocular Fundus Diseases, 2016, 32(5): 518-521. doi: 10.3760/cma.j.issn.1005-1015.2016.05.014 Copy

1.	Bone RA, Landrum JT, Tarsis SL. Preliminary identification of the human macular pigment[J]. Vision Res,1985,25(11):1531-1535.
2.	Snodderly DM, Brown PK, Delori FC, et al. The macular pigment. Ⅰ. Absorbance spectra, localization, and discrimination from other yellow pigments in primate retinas[J]. Invest Ophthalmol Vis Sci,1984,25(6):660-673.
3.	Khachik F, Bernstein PS, Garland DL. Identification of lutein and zeaxanthin oxidation products in human and monkey retinas[J]. Invest Ophthalmol Vis Sci,1997,38(9):1802-1811.
4.	Haegerstrom-Portnoy G. Short-wavelength-sensitive-cone sensitivity loss with aging:a protective role for macular pigment?[J]. J Opt Soc Am A,1988,5(12):2140-2144.
5.	Wooten BR, Hammond BR. Macular pigment:influences on visual acuity and visibility[J]. Prog Retin Eye Res,2002,21(2):225-240.
6.	Seddon JM, Ajani UA, Sperduto RD, et al. Dietary carotenoids, vitamins A, C, and E, and advanced age-related macular degeneration[J]. JAMA,1994,272(18):1413-1420.
7.	Bhosale P, Larson AJ, Frederick JM, et al. Identification and characterization of a Pi isoform of glutathione S-transferase (GSTP1) as a zeaxanthin-binding protein in the macula of the human eye[J]. J Biol Chem,2004,279(47):49447-49454.DOI:10.1074/jbc.M405334200.
8.	Yemelyanov AY, Katz NB, Bernstein PS. Ligand-binding characterization of xanthophyll carotenoids to solubilized membrane proteins derived from human retina[J]. Exp Eye Res,2001,72(4):381-392.DOI:10.1006/exer.2000.0965.
9.	黄绯绯, 林晓明. 老年黄斑变性患者与对照人群膳食、血清叶黄素/玉米黄素以及血脂水平的比较[J]. 北京大学学报(医学版),2014,46(2):237-241. DOI:10.3969/j.issn.1671-167X.2014.02.012. Huang FF,Lin XM.Comparison of daily intake of lutein +zeaxanthin,serum concentration of lutein/zeaxanthin and lipids profile between age-related macular degeneration patients and controls HUANG[J].Jounal of Peking University(Health Sciences),2014,46(2):237-241. DOI:10.3969/j.issn.1671-167X.2014.02.012.
10.	Eye Disease Case-Control Study Group.Antioxidant status and neovascular age-related macular degeneration[J]. Arch Ophthalmol,1993,111(1):104-109.
11.	Zhou H, Zhao X, Johnson EJ, et al. Serum carotenoids and risk of age-related macular degeneration in a chinese population sample[J]. Invest Ophthalmol Vis Sci,2011,52(7):4338-4344.doi:10.1167/iovs.10-6519.
12.	Bone RA, Landrum JT, Guerra LH, et al. Lutein and zeaxanthin dietary supplements raise macular pigment density and serum concentrations of these carotenoids in humans[J]. J Nutr,2003,133(4):992-998.
13.	Iannaccone A, Mura M, Gallaher KT, et al. Macular pigment optical density in the elderly:findings in a large biracial Midsouth population sample[J]. Invest Ophthalmol Vis Sci,2007,48(4):1458-1465.DOI:10.1167/iovs.06-0438.
14.	Johnson EJ, Chung HY, Caldarella SM, et al. The influence of supplemental lutein and docosahexaenoic acid on serum, lipoproteins, and macular pigmentation[J]. Am J Clin Nutr,2008,87(5):1521-1529.
15.	Bone RA, Landrum JT. Dose-dependent response of serum lutein and macular pigment optical density to supplementation with lutein esters[J]. Arch Biochem Biophys,2010,504(1):50-55.DOI:10.1016/j.abb.2010.06.019.
16.	Weigert G, Kaya S, Pemp B, et al. Effects of lutein supplementation on macular pigment optical density and visual acuity in patients with age-related macular degeneration[J]. Invest Ophthalmol Vis Sci,2011,52(11):8174-8178.DOI:10.1167/iovs.11-7522.
17.	Landrum J, Bone R, Mendez V, et al. Comparison of dietary supplementation with lutein diacetate and lutein:a pilot study of the effects on serum and macular pigment[J]. Acta Biochim Pol,2012,59(1):167-169.
18.	Garcia-Layana A, Recalde S, Alaman AS, et al. Effects of lutein and docosahexaenoic Acid supplementation on macular pigment optical density in a randomized controlled trial[J]. Nutrients,2013,5(2):543-551.DOI:10.3390/nu5020543.
19.	Dawczynski J, Jentsch S, Schweitzer D, et al. Long term effects of lutein, zeaxanthin and omega-3-LCPUFAs supplementation on optical density of macular pigment in AMD patients:the LUTEGA study[J]. Graefe's Arch Clin Exp Ophthalmol,2013,251(12):2711-2723.DOI:10.1007/s00417-013-2376-6.
20.	Huang YM, Yan SF, Ma L, et al. Serum and macular responses to multiple xanthophyll supplements in patients with early age-related macular degeneration[J]. Nutrition,2013,29(2):387-392.DOI:10.1016/j.nut.2012.06.009.
21.	Sabour-Pickett S, Beatty S, Connolly E, et al. Supplementation with three different macular carotenoid formulations in patients with early age-related macular degeneration[J]. Retina,2014,34(9):1757-1766. DOI:10.1097/IAE.0000000000000174.
22.	Huang YM, Dou HL, Huang FF, et al. Changes following supplementation with lutein and zeaxanthin in retinal function in eyes with early age-related macular degeneration:a randomised, double-blind, placebo-controlled trial[J]. Br J Ophthalmol,2015,99(3):371-375.DOI:10.1136/bjophthalmol-2014-305503.
23.	Obana A, Tanito M, Gohto Y, et al. Changes in macular pigment optical density and serum lutein concentration in japanese subjects taking two different lutein supplements. PLoS One,2015,10(10):139257. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4599964/.DOI:10.1371/journal.pone.0139257.
24.	Bone RA, Landrum JT, Friedes LM, et al. Distribution of lutein and zeaxanthin stereoisomers in the human retina[J]. Exp Eye Res,1997,64(2):211-218.DOI:10.1006/exer.1996.0210.
25.	Nolan JM, Stack J, O'Connell E, et al. The relationships between macular pigment optical density and its constituent carotenoids in diet and serum[J]. Invest Ophthalmol Vis Sci,2007,48(2):571-582.DOI:10.1167/iovs.06-0864.
26.	Broekmans WM, Berendschot TT, Klopping-Ketelaars IA, et al. Macular pigment density in relation to serum and adipose tissue concentrations of lutein and serum concentrations of zeaxanthin[J]. Am J Clin Nutr,2002,76(3):595-603.
27.	Landrum JT, Bone RA, Joa H, et al. A one year study of the macular pigment:the effect of 140 days of a lutein supplement[J]. Exp Eye Res, 1997, 65(1):57-62. DOI:10.1006/exer.1997.0309.
28.	LaRowe TL, Mares JA, Snodderly DM, et al. Macular pigment density and age-related maculopathy in the Carotenoids in Age-Related Eye Disease Study:an ancillary study of the women's health initiative[J]. Ophthalmology, 2008, 115(5):876-883.DOI:10.1016/j.ophtha.2007.06.015.

1. Bone RA, Landrum JT, Tarsis SL. Preliminary identification of the human macular pigment[J]. Vision Res,1985,25(11):1531-1535.
2. Snodderly DM, Brown PK, Delori FC, et al. The macular pigment. Ⅰ. Absorbance spectra, localization, and discrimination from other yellow pigments in primate retinas[J]. Invest Ophthalmol Vis Sci,1984,25(6):660-673.
3. Khachik F, Bernstein PS, Garland DL. Identification of lutein and zeaxanthin oxidation products in human and monkey retinas[J]. Invest Ophthalmol Vis Sci,1997,38(9):1802-1811.
4. Haegerstrom-Portnoy G. Short-wavelength-sensitive-cone sensitivity loss with aging:a protective role for macular pigment?[J]. J Opt Soc Am A,1988,5(12):2140-2144.
5. Wooten BR, Hammond BR. Macular pigment:influences on visual acuity and visibility[J]. Prog Retin Eye Res,2002,21(2):225-240.
6. Seddon JM, Ajani UA, Sperduto RD, et al. Dietary carotenoids, vitamins A, C, and E, and advanced age-related macular degeneration[J]. JAMA,1994,272(18):1413-1420.
7. Bhosale P, Larson AJ, Frederick JM, et al. Identification and characterization of a Pi isoform of glutathione S-transferase (GSTP1) as a zeaxanthin-binding protein in the macula of the human eye[J]. J Biol Chem,2004,279(47):49447-49454.DOI:10.1074/jbc.M405334200.
8. Yemelyanov AY, Katz NB, Bernstein PS. Ligand-binding characterization of xanthophyll carotenoids to solubilized membrane proteins derived from human retina[J]. Exp Eye Res,2001,72(4):381-392.DOI:10.1006/exer.2000.0965.
9. 黄绯绯, 林晓明. 老年黄斑变性患者与对照人群膳食、血清叶黄素/玉米黄素以及血脂水平的比较[J]. 北京大学学报(医学版),2014,46(2):237-241. DOI:10.3969/j.issn.1671-167X.2014.02.012. Huang FF,Lin XM.Comparison of daily intake of lutein +zeaxanthin,serum concentration of lutein/zeaxanthin and lipids profile between age-related macular degeneration patients and controls HUANG[J].Jounal of Peking University(Health Sciences),2014,46(2):237-241. DOI:10.3969/j.issn.1671-167X.2014.02.012.
10. Eye Disease Case-Control Study Group.Antioxidant status and neovascular age-related macular degeneration[J]. Arch Ophthalmol,1993,111(1):104-109.
11. Zhou H, Zhao X, Johnson EJ, et al. Serum carotenoids and risk of age-related macular degeneration in a chinese population sample[J]. Invest Ophthalmol Vis Sci,2011,52(7):4338-4344.doi:10.1167/iovs.10-6519.
12. Bone RA, Landrum JT, Guerra LH, et al. Lutein and zeaxanthin dietary supplements raise macular pigment density and serum concentrations of these carotenoids in humans[J]. J Nutr,2003,133(4):992-998.
13. Iannaccone A, Mura M, Gallaher KT, et al. Macular pigment optical density in the elderly:findings in a large biracial Midsouth population sample[J]. Invest Ophthalmol Vis Sci,2007,48(4):1458-1465.DOI:10.1167/iovs.06-0438.
14. Johnson EJ, Chung HY, Caldarella SM, et al. The influence of supplemental lutein and docosahexaenoic acid on serum, lipoproteins, and macular pigmentation[J]. Am J Clin Nutr,2008,87(5):1521-1529.
15. Bone RA, Landrum JT. Dose-dependent response of serum lutein and macular pigment optical density to supplementation with lutein esters[J]. Arch Biochem Biophys,2010,504(1):50-55.DOI:10.1016/j.abb.2010.06.019.
16. Weigert G, Kaya S, Pemp B, et al. Effects of lutein supplementation on macular pigment optical density and visual acuity in patients with age-related macular degeneration[J]. Invest Ophthalmol Vis Sci,2011,52(11):8174-8178.DOI:10.1167/iovs.11-7522.
17. Landrum J, Bone R, Mendez V, et al. Comparison of dietary supplementation with lutein diacetate and lutein:a pilot study of the effects on serum and macular pigment[J]. Acta Biochim Pol,2012,59(1):167-169.
18. Garcia-Layana A, Recalde S, Alaman AS, et al. Effects of lutein and docosahexaenoic Acid supplementation on macular pigment optical density in a randomized controlled trial[J]. Nutrients,2013,5(2):543-551.DOI:10.3390/nu5020543.
19. Dawczynski J, Jentsch S, Schweitzer D, et al. Long term effects of lutein, zeaxanthin and omega-3-LCPUFAs supplementation on optical density of macular pigment in AMD patients:the LUTEGA study[J]. Graefe's Arch Clin Exp Ophthalmol,2013,251(12):2711-2723.DOI:10.1007/s00417-013-2376-6.
20. Huang YM, Yan SF, Ma L, et al. Serum and macular responses to multiple xanthophyll supplements in patients with early age-related macular degeneration[J]. Nutrition,2013,29(2):387-392.DOI:10.1016/j.nut.2012.06.009.
21. Sabour-Pickett S, Beatty S, Connolly E, et al. Supplementation with three different macular carotenoid formulations in patients with early age-related macular degeneration[J]. Retina,2014,34(9):1757-1766. DOI:10.1097/IAE.0000000000000174.
22. Huang YM, Dou HL, Huang FF, et al. Changes following supplementation with lutein and zeaxanthin in retinal function in eyes with early age-related macular degeneration:a randomised, double-blind, placebo-controlled trial[J]. Br J Ophthalmol,2015,99(3):371-375.DOI:10.1136/bjophthalmol-2014-305503.
23. Obana A, Tanito M, Gohto Y, et al. Changes in macular pigment optical density and serum lutein concentration in japanese subjects taking two different lutein supplements. PLoS One,2015,10(10):139257. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4599964/.DOI:10.1371/journal.pone.0139257.
24. Bone RA, Landrum JT, Friedes LM, et al. Distribution of lutein and zeaxanthin stereoisomers in the human retina[J]. Exp Eye Res,1997,64(2):211-218.DOI:10.1006/exer.1996.0210.
25. Nolan JM, Stack J, O'Connell E, et al. The relationships between macular pigment optical density and its constituent carotenoids in diet and serum[J]. Invest Ophthalmol Vis Sci,2007,48(2):571-582.DOI:10.1167/iovs.06-0864.
26. Broekmans WM, Berendschot TT, Klopping-Ketelaars IA, et al. Macular pigment density in relation to serum and adipose tissue concentrations of lutein and serum concentrations of zeaxanthin[J]. Am J Clin Nutr,2002,76(3):595-603.
27. Landrum JT, Bone RA, Joa H, et al. A one year study of the macular pigment:the effect of 140 days of a lutein supplement[J]. Exp Eye Res, 1997, 65(1):57-62. DOI:10.1006/exer.1997.0309.
28. LaRowe TL, Mares JA, Snodderly DM, et al. Macular pigment density and age-related maculopathy in the Carotenoids in Age-Related Eye Disease Study:an ancillary study of the women's health initiative[J]. Ophthalmology, 2008, 115(5):876-883.DOI:10.1016/j.ophtha.2007.06.015.

Chinese Journal of Ocular Fundus Diseases

Relationship between macular pigment optical density and serum concentration of lutein and zeaxanthin in an adult population

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content