Integrins Mediate the Migration of HepG2 Cells Induced by Low Shear Stress_Journal of Biomedical Engineering

Authors：

WANGLijuan ,  LIUXiaoheng , YUHongchi , ZHOUFating , CHENHuilin , LIUQianqi

Institute of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China;

Corresponding author：

LIUXiaoheng, Email: liuxiaohg@scu.edu.cn

Keywords：

low shear stress; cell migration; integrins; hepatomal cell

DOI：

10.7507/1001-5515.20140063

Video：

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Abstract Full text Figures/Tables Video References Cited by

Low shear stress is a component of the tumor microenvironment in vivo and plays a key role in regulating cancer cell migration and invasion. The integrin, as a mechano-sensors mediating and integrating mechanical and chemical signals, induce the adhesion between cells and extracellular matrix (ECM). The purpose of this study is to investigate the effect of low shear stress(1.4 dyn/cm²)on the migration of HepG2 cells and the expression of integrin. Scratch wound migration assay was performed to examine the effect of low shear stress on the migration of HepG2 cells at 0 h, 1 h, 2 h and 4 h, respectively. F-actin staining was used to detect the expression of F-actin in HepG2 cells treated with low shear stress at 2 h and 4 h. Western blot analysis was carried out to determine the effect of low shear stress on the expression of integrin at different durations. The results showed that the migrated distance of HepG2 cells and the expression of F-actin increased significantly compared with the controls. The integrin α subunits showed a different time-dependent expression, suggesting that various subunits of integrin exhibit different effects in low shear stress regulating cancer cells migration.

Citation： WANGLijuan, LIUXiaoheng, YUHongchi, ZHOUFating, CHENHuilin, LIUQianqi. Integrins Mediate the Migration of HepG2 Cells Induced by Low Shear Stress. Journal of Biomedical Engineering, 2014, 31(2): 336-340. doi: 10.7507/1001-5515.20140063 Copy

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1. FUKUMURA D,JAIN R K.Tumor microenvironment abnormalities:causes,consequences,and strategies to normalize[J].J Cell Biochem,2007,101(4):937-949.
2. TLSTY T C.Tumor stroma and regulation of cancer development[J].Annu Rev Pathol,2006,1:119-150.
3. JOYCE J A,POLLARD J W.Microenvironmental regulation of metastasis[J].Nat Rev Cancer,2009,9(4):239-252.
4. POLACHECK W J,CHAREST J L,KAMM R D.Interstitial flow influences direction of tumor cell migration through competing mechanisms[J].Proc Natl Acad Sci U S A,2011,108(27):11115-11120.
5. MITCHELL M J,KING M R.Computational and experimental models of cancer cell response to fluid shear stress[J].Front Oncol,2013,3:44.
6. GOMEZ E W,CHEN Q K,GJOREVSKI N,et al.Tissue geometry patterns epithelial-mesenchymal transition via intercellular mechanotransduction[J].J Cell Biochem,2010,110(1):44-51.
7. BIANCO A,POUKKULA M,CLIFFE A,et al.Two distinct modes of guidance signalling during collective migration of border cells[J].Nature,2007,448(7151):362-365.
8. SHIELDS J F,YONG C,TOMEI A A,et al.Autologous chemotaxis as a mechanism of tumor cell homing to lymphatics via interstitial flow and autocrine CCR7 signaling[J].Cancer Cell,2007,11(6):526-538.
9. QAZI H,SHI Z D,TARBELL J M.Fluid shear stress regulates the invasive potential of glioma cells via modulation of migratory activity and matrix metalloproteinase expression[J].PLoS One,2011,6(5):e20348.
10. ROZSA D,TRENCSENYI G,KERTAI P,et al.Lymphatic spread of mesenchymal renal tumor to metastatic parathymic lymph nodes in rat[J].Histol Histopathol,2009,24(11):1367-1379.
11. SHIEH A C,ROZANSKY H A,HINZ B,et al.Tumor cell invasion is promoted by interstitial flow-induced matrix priming by stromal fibroblasts[J].Cancer Res,2011,71(3):790-800.
12. KANNO S,WU Y J,LEE P C,et al.Angiotensin-converting enzyme inhibitor preserves p21 and endothelial nitric oxide synthase expression in monocrotaline-induced pulmonary arterial hypertension in rats[J].Circulation,2001,104(8):945-950.
13. DAVIDSON L A,MARSDEN M,KELLER R,et al.Integrin alpha5beta1 and fibronectin regulate polarized cell protrusions required for Xenopus convergence and extension[J].Curr Biol,2006,16(9):833-844.
14. KUMAR S,WEAVER V M.Mechanics,malignancy,and metastasis:the force journey of a tumor cell[J].Cancer Metastasis Rev,2009,28(1-2):113-127.
15. NURMENNIEMI S,SINIKUMPU T,ALAHUHTA I,et al.A novel organotypic model mimics the tumor microenvironment[J].Am J Pathol,2009,175(3):1281-1291.
16. RESTO V A,BURDICK M M,DAGIA N M,et al.L-selectin-mediated lymphocyte-cancer cell interactions under low fluid shear conditions[J].J Biol Chem,2008,283(23):15816-15824.
17. ZEBDA N,DUBROVSKYI O,BIRUKOV K G.Focal adhesion kinase regulation of mechanotransduction and its impact on endothelial cell functions[J].Microvasc Res,2012,83(1):71-81.

Journal of Biomedical Engineering

Integrins Mediate the Migration of HepG2 Cells Induced by Low Shear Stress

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