Preparation of Elastic Porous Cell Scaffold Fabricated with Combined Polydimethylsiloxane (PDMS) and Hydroxyapatite (HA)_Journal of Biomedical Engineering

Authors：

YANGYang ¹ , LANDing ¹ , HUANGYan ² , LIYanming ¹ ,  WANGYuren ¹ , SUNLianwen ² , FANYubo ²

1. National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Science, Beijing 100190, China;
2. School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China;

Corresponding author：

WANGYuren, Email: yurenwang@imech.ac.cn

Keywords：

elastic; cell scaffold; porosity; biocompatibility; rat bone marrow derived mesenchymal stem cell

DOI：

10.7507/1001-5515.20140117

Video：

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Polydimethylsiloxane (PDMS) and hydroxyapatite (HA) were combined in our laboratory to fabricate an elastic porous cell scaffold with pore-forming agent, and then the scaffold was used as culture media for rat bone marrow derived mesenchymal stem cells (rBMSCs). Different porous materials (square and circular in shape) were prepared by different pore-forming agents (NaCl or paraffin spheres) with adjustable porosity (62%-76%). The HA crystals grew on the wall of hole when the material was exposed to SBF solutions, showing its biocompatibility and ability to support the cells to attach on the materials.

Citation： YANGYang, LANDing, HUANGYan, LIYanming, WANGYuren, SUNLianwen, FANYubo. Preparation of Elastic Porous Cell Scaffold Fabricated with Combined Polydimethylsiloxane (PDMS) and Hydroxyapatite (HA). Journal of Biomedical Engineering, 2014, 31(3): 625-631. doi: 10.7507/1001-5515.20140117 Copy

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1. SONG G, JU Y, SHEN X, et al. Mechanical stretch promotes proliferation of rat bone marrow mesenchymal stem cells[J]. Colloids Surf B Biointerfaces, 2007, 58(2):271-277.
2. TANG L L, WANG Y L, PAN J, et al. The effect of step-wise increased stretching on rat calvarial osteoblast collagen production[J]. J Biomech, 2004, 37(1):157-161.
3. YANG Y, MAGNAY J, COOLING L, et al. Effects of substrate characteristics on bone cell response to the mechanical environment[J]. Med Biol Eng Comput, 2004, 42(1):22-29.
4. JANSEN J H, WEYTS F A, WESTBROEK I, et al. Stretch-induced phosphorylation of ERK1/2 depends on differentiation stage of osteoblasts[J]. J Cell Biochem, 2004, 93(3):542-551.
5. 吕守芹,杨帆,龙勉.细胞-分子生物力学研究进展[J].医用生物力学,2009,24(2):79-84.
6. 黄艳,樊瑜波.力学因素影响骨髓间充质干细胞活性的研究进展[J].生物医学工程学杂志,2010,27(4):920-923.
7. DROTLEFF S, LUNGWITZ U, BREUNIG M, et al. Biomimetic polymers in pharmaceutical and biomedical sciences[J]. Eur J Pharm Biopharm, 2004, 58(2):385-407.
8. TIRELLI N, LUTOLF M P, NAPOLI A, et al. Poly(ethylene glycol) block copolymers[J]. J Biotechnol, 2002, 90(1):3-15.
9. BERGER J, REIST M, MAYER J M, et al. Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications[J]. Eur J Pharm Biopharm, 2004, 57(1):19-34.
10. 崔福斋,郭牧遥.生物陶瓷材料的应用及其发展前景[J].药物分析杂志,2010,30(7):1343-1347.
11. 包崇云,张兴栋.磷酸钙生物材料固有骨诱导性的研究现状与展望[J].生物医学工程学杂志,2006,23(2):442-445,454.
12. 冯庆玲,崔福斋,张伟.纳米羟基磷灰石/胶原骨修复材料[J].中国医学科学院学报,2002,24(2):124-128.
13. ZHANG K, WANG Y, HILLMYER M A, et al. Processing and properties of porous poly(L-lactide)/bioactive glass composites[J]. Biomaterials, 2004, 25(13):2489-2500.
14. YAO J, RADIN S, LEBOY P S, et al. The effect of bioactive glass content on synthesis and bioactivity of composite poly(lactic-co-glycolic acid)/bioactive glass substrate for tissue engineering[J]. Biomaterials, 2005, 26(14):1935-1943.
15. KATANOSAKA Y, BAO J H, KOMATSU T, et al. Analysis of cyclic-stretching responses using cell-adhesion-patterned cells[J]. J Biotechnol, 2008, 133(1):82-89.
16. WANG J G, MIYAZU M, XIANG P, et al. Stretch-induced cell proliferation is mediated by FAK-MAPK pathway[J]. Life Sci, 2005, 76(24):2817-2825.
17. KOKUBO T, TAKADAMA H. How useful is SBF in predicting in vivo bone bioactivity?[J]. Biomaterials, 2006, 27(15):2907-2915.
18. HUANG Y, JIA X, BAI K, et al. Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem cells[J]. Arch Med Res, 2010, 41(7):497-505.

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