Primary Culture of Adipose-derived Stromal Cells_Journal of Biomedical Engineering

Authors：

DENGChunying ,  LIUBin , ZHANGJinxia

First Department of Neurology, the Affiliated Hospital of Hebei United University, Tangshan 063000, China;

Corresponding author：

LIUBin, Email: liubintsh@126.com

Keywords：

adipose-derived stromal cell; primary culture; fat; rat

DOI：

10.7507/1001-5515.20140158

Video：

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Abstract Full text Figures/Tables Video References Cited by

This study was aimed to explore the methodology regarding culture, proliferation and purification of adipose-derived stromal cells (ADSCs), and to study their biological characteristics. ADSCs were obtained using type Ⅰ collagenase digestion method. Cell growth was observed, and cell viability were detected under different digestion period by MTT. The ADSCs were then identified and induced. The results showed that adherent cells digested by type Ⅰ collagenase for 60 min had a strong proliferation capability. After the induction of different inducers these adherent cells could differentiate into nerve cells and fat cells. The best digestion period was proved to be of 60 minutes in the experiment. The results indicate that stem cells with multilineage differentiation ability could be separated from adipose tissue, namely ADSCs.

Citation： DENGChunying, LIUBin, ZHANGJinxia. Primary Culture of Adipose-derived Stromal Cells. Journal of Biomedical Engineering, 2014, 31(4): 842-845. doi: 10.7507/1001-5515.20140158 Copy

1.	ZHANG L, ZHANG HT, HONG SQ, et al. Cografted Wharton's Jelly cells-derived neurospheres and BDNF promote functional recovery after rat spinal cord transaction[J]. Neurochem Res, 2009,34(11): 2030-2039.
2.	PETSA A, GARGANI S, FELESAKIS A, et al. Effectiveness of protocol for the isolation of Wharton's Jelly stem cells in large-scale applications[J].In Vitro Cell Dev Biol Anim,2009,45(10): 573-576.
3.	TOMAR G B,SRIVASTAVA R K,GUPTA N, et al. Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine[J]. Biochem Biophys Res Commun,2010,393(3):377-383.
4.	GIMBLE J M, KATZ A J, BUNNELL B A. Adipose-derived stem cells for regenerative medicine[J].Circ Res,2007,100(9):1249-1260.
5.	刘斌,吴孟海,董静,等.脂肪基质细胞诱导分化为神经元细胞的实验[J].中国组织工程研究与临床康复,2011,14(1):15-18.
6.	中华人民共和国科学技术部.关于发布《关于善待实验动物的指导意见》的通知[EB/OL].(2006-09-30)[2013-02-04] .http://www.most.gov.cn/fggw/zfwj/zfwj2006/200609/t20060930_54389.htm.
7.	YOSHIMURA K, SUGA H, ETO H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation[J].Regen Med,2009,4(2):265-273.
8.	VAN HARMELEN V, SKURK T, HAUNER H. Primary culture and differentiation of human adipocyte precursor cells[J].Methods Mol Med,2005,107:125-135.
9.	HOUGHTON A, OYAJOBI B O, FOSTER G A, et al. Immortalization of human marrow stromal cells by retroviral transduction with a temperature sensitive oncogene: identification of bipotential precursor cells capable of directed differentiation to either an osteoblast or adipocyte phenotype[J].Bone,1998,22(1):7-16.
10.	MERCERON C, PORTRON S, MASSON M, et al. Cartilage tissue engineering: From hydrogel to mesenchymal stem cells[J].Biomed Mater Eng,2010,20(3):159-166.
11.	CHEN Q, YANG Z, SUN S, et al. Adipose-derived stem cells modified genetically in vivo promote reconstruction of bone defects[J]. Cytotherapy, 2010,12(6):831-840.
12.	HEYDARKHAN-HAGVALL S, SCHENKE-LAYLAND K, YANG J Q, et al. Human adipose stem cells: a potential cell source for cardiovascular tissue engineering[J]. Cells Tissues Organs,2008,187(4):263-274.

1. ZHANG L, ZHANG HT, HONG SQ, et al. Cografted Wharton's Jelly cells-derived neurospheres and BDNF promote functional recovery after rat spinal cord transaction[J]. Neurochem Res, 2009,34(11): 2030-2039.
2. PETSA A, GARGANI S, FELESAKIS A, et al. Effectiveness of protocol for the isolation of Wharton's Jelly stem cells in large-scale applications[J].In Vitro Cell Dev Biol Anim,2009,45(10): 573-576.
3. TOMAR G B,SRIVASTAVA R K,GUPTA N, et al. Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine[J]. Biochem Biophys Res Commun,2010,393(3):377-383.
4. GIMBLE J M, KATZ A J, BUNNELL B A. Adipose-derived stem cells for regenerative medicine[J].Circ Res,2007,100(9):1249-1260.
5. 刘斌,吴孟海,董静,等.脂肪基质细胞诱导分化为神经元细胞的实验[J].中国组织工程研究与临床康复,2011,14(1):15-18.
6. 中华人民共和国科学技术部.关于发布《关于善待实验动物的指导意见》的通知[EB/OL].(2006-09-30)[2013-02-04] .http://www.most.gov.cn/fggw/zfwj/zfwj2006/200609/t20060930_54389.htm.
7. YOSHIMURA K, SUGA H, ETO H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation[J].Regen Med,2009,4(2):265-273.
8. VAN HARMELEN V, SKURK T, HAUNER H. Primary culture and differentiation of human adipocyte precursor cells[J].Methods Mol Med,2005,107:125-135.
9. HOUGHTON A, OYAJOBI B O, FOSTER G A, et al. Immortalization of human marrow stromal cells by retroviral transduction with a temperature sensitive oncogene: identification of bipotential precursor cells capable of directed differentiation to either an osteoblast or adipocyte phenotype[J].Bone,1998,22(1):7-16.
10. MERCERON C, PORTRON S, MASSON M, et al. Cartilage tissue engineering: From hydrogel to mesenchymal stem cells[J].Biomed Mater Eng,2010,20(3):159-166.
11. CHEN Q, YANG Z, SUN S, et al. Adipose-derived stem cells modified genetically in vivo promote reconstruction of bone defects[J]. Cytotherapy, 2010,12(6):831-840.
12. HEYDARKHAN-HAGVALL S, SCHENKE-LAYLAND K, YANG J Q, et al. Human adipose stem cells: a potential cell source for cardiovascular tissue engineering[J]. Cells Tissues Organs,2008,187(4):263-274.

Journal of Biomedical Engineering

Primary Culture of Adipose-derived Stromal Cells

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