Cytotoxicity <i>in Vitro</i> of a Noval Ni-free ZrCuFeAlAg Bulk Metallic Glass_Journal of Biomedical Engineering

Authors：

PANGHuifang ^1,2 , XUCong ¹ , QINHua ¹ , LIDemin ¹ , LIPeiyuan ¹ , WANGBo ¹ , ZHANGShujuan ¹ ,  ZHAOQiu ¹

1. Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;
2. Institute of Digestive Disease, The Second Affiliated Hospital, Baotou Medical college, Baotou 014030, China;

Corresponding author：

ZHAOQiu, Email: zhaoqiu@medmail.com

Keywords：

Zr-based bulk metallic glass; cytotoxicity; biocompatibility; MG-63 cell

DOI：

10.7507/1001-5515.20150069

Video：

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This paper is to evaluate the biocompatibility and cytotoxicity of a new Ni-free Zr-based bulk metallic glass (BMG), Zr_60.14Cu_22.31Fe_4.85Al_9.7Ag₃, by comparing it with conventional Ti6Al4V alloy. According to ISO 10993-5:1999 and GB/T 16886.5-1997 standards, Zr_60.14Cu_22.31Fe_4.85Al_9.7Ag₃, pure Zr and Ti6Al4V materials were extracted with surface area of sample/volume of medium ratio being 1 cm²/mL and 0.5 cm²/mL, respectively. The viabilities of MG-63 cells (Human osteosarcoma cell line) cultured in the BMG medium extracts for 1, 3 and 5 days were determined by CCK-8 assay. The cellular morphology of MG-63 cells cultured on the surface of samples for 3 days was tested through laser scanning confocal microscopy (LSCM) and scanning electron microscopy (SEM). The relative growth rate (RGR) of MG-63 cells cultured in Zr_60.14Cu_22.31 Fe_4.85 Al_9.7Ag₃ and pure Zr were both more than 85%, indicating that the cytotoxicity of BMG was relatively low and met the national biomedical material eligibility standard. There was insignificant difference in the morphology of MG-63 cells cultured in the BMG medium extracts and the control group through LSCM and SEM, which showed the BMG had excellent biological compatibility. The Zr-based bulk metallic glass Zr_60.14Cu_22.31Fe_4.85Al_9.7Ag₃ and the conventional Ti6Al4V alloy both had no obvious cytotoxicity to MG-63 cells. These results provided evidence that the new Zr-based bulk metallic glass could be potential replacement material for the orthopedic surgical implant.

Citation： PANGHuifang, XUCong, QINHua, LIDemin, LIPeiyuan, WANGBo, ZHANGShujuan, ZHAOQiu. Cytotoxicity in Vitro of a Noval Ni-free ZrCuFeAlAg Bulk Metallic Glass. Journal of Biomedical Engineering, 2015, 32(2): 380-386. doi: 10.7507/1001-5515.20150069 Copy

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24.	GEETHA M, SINGH A K, ASOKAMANI R, et al. Ti based biomaterials, the ultimate choice for orthopaedic implants-A review[J]. Prog Mater Sci, 2009, 54(3):397-425.

1. 李瑞, 王青山.生物材料生物相容性的评价方法和发展趋势[J].中国组织工程研究与临床康复, 2011, 15(29):5471-5474.
2. 李红梅, 雷霆, 方树铭, 等.生物医用钛合金的研究进展[J].金属功能材料, 2011, 18(2):70-73.
3. 胡欣, 魏强, 刘霜, 等.口腔修复用钛锆铌锡合金机械性能及体内外的生物毒性[J].中国组织工程研究与临床康复, 2009, 13(29):5627-5631.
4. LI Y, WONG C, XIONG J, et al. Cytotoxicity of Titanium and Titanium alloying elements[J]. J Dent Res, 2010, 89(5):493-497.
5. GORDIN D M, GLORIANT T, NEMTOI G, et al. Synthesis, structure and electrochemical behavior of a beta Ti-12Mo-5Ta alloy as new biomaterial[J]. Mater Lett, 2005, 59(23):2936-2941.
6. 张明华, 于振涛, 张殿忠, 等.β型钛合金材料的生物相容性评价[J].中国组织工程研究与临床康复, 2010, 14(42):7849-7853.
7. 吴桐.浅谈几种生物医用高分子材料的应用[J].科技资讯, 2011, (29):52-52.
8. 胡欣, 魏强, 李长义, 等.新型口腔修复用钛锆铌锡合金的生物安全性评价[J].口腔颌面修复学杂志, 2010, 11(1):33-36.
9. OLIVEIRA N T, ALEIXO G, CARAM R A. Development of Ti-Mo alloys for biomedical applications:Microstructure and electrochemical characterization[J]. Materials Science and Engineering:A, 2007, 452:727-731.
10. MORRISON M L, BUCHANAN R A, LEON R V, et al. The electrochemical evaluation of a Zr-based bulk metallic glass in a phosphate-buffered saline electrolyte[J]. J Biomed Mater Res A, 2005, 74(3):430-438.
11. ZHANG Z Y, KEPPENS V, LIAW P K, et al. Elastic properties of Zr-based bulk metallic glasses studied by resonant ultrasound spectroscopy[J]. J Mater Res, 2007, 22:364-367.
12. BUZZI S, JIN K F, UGGOWITZER P J, et al. Cytotoxicity of Zr-based bulk metallic glasses[J]. Intermetallics, 2006, 14(7):729-734.
13. LIU L, QIU C L, CHEN Q, et al. Deformation behavior, corrosion resistance, and cytotoxicity of Ni-free Zr-based bulk metallic glasses[J]. J Biomed Mater Res A, 2008, 86(1):160-169.
14. WANG G Y, LIAW P K, MORRISION M L. Progress in studying the fatigue behavior of Zr-based bulk-metallic glasses, their composites[J]. Intermetallics, 2009, 17(8):579-590.
15. LIU L, QIU C L, HUANG C Y, et al. Biocompatibility of Ni-free Zr-based bulk metallic glasses[J]. Intermetallics, 2009, 17(4):235-240.
16. HUANG L, YOKOYAMA Y, WU W, et al. Ni-free Zr-Cu-Al-Nb-Pd bulk metallic glasses with different Zr/Cu ratios for biomedical applications[J]. J Biomed Mater Res Part B, 2012, 100(6):1472-1482.
17. HUANG L, QIAO D C, GREEN B A, et al. Bio-corrosion study on Zr-based bulk metallic glasses[J]. Intermetallics, 2009, 17(4):195-199.
18. HUANG L, CAO Z, MEYER H M, et al. Responses of bone-forming cells on pre-immersed Zr-based bulk metallic glasses:Effects of composition and roughness[J]. Acta Biomater, 2011, 7(1):395-405.
19. YASZEMSK M J, TRANTOLO D J, LEWANDROWSKI K, et al. Biomaterials in orthopedics[M].New York:Marcel Dekker, 2004.
20. ISO-10993-5:Biological evaluation of medical devices-Part 5:tests for cytotoxicity:in vitro methods[S]. VA:ANSI/AAMI, 1999.
21. 国家标准局信息分类编码研究所.GB/T 16886.5:医疗器械生物学评价-第五部分:细胞毒性试验:体外法[S].北京:中国标准出版社, 1997.
22. DEMETRIOUS M D, WIEST A, HOFMANN D C, et al. Amorphous metals for hard-tissue prosthesis[J]. Journal of the Minerals Metals and Materials Society, 2010, 62(6):83-91.
23. LUO J, DUAN H P, MA C L, et al. Effects of Yttrium and Erbium additions on glass-forming ability and mechanical properties of bulk glassy Zr-Al-Ni-Cu alloys[J]. Mater Trans, 2006, 47(2):450-453.
24. GEETHA M, SINGH A K, ASOKAMANI R, et al. Ti based biomaterials, the ultimate choice for orthopaedic implants-A review[J]. Prog Mater Sci, 2009, 54(3):397-425.

Journal of Biomedical Engineering

Cytotoxicity in Vitro of a Noval Ni-free ZrCuFeAlAg Bulk Metallic Glass

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