Development of Silk Fibroin Composite Films for Corneal Repair_Journal of Biomedical Engineering

Authors：

ZHANGShanshan ¹ , LIJiaojiao ¹ , ZHANGFang ¹ , ZHANGXiaofeng ² ,  LUShenzhou ¹

1. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China;
2. Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;

Corresponding author：

LUShenzhou, Email: lushenzhou@suda.edu.cn

Keywords：

silk fibroin; cornea; composite membrane; transparency; cell compatibility

DOI：

10.7507/1001-5515.20150155

Video：

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It is important to design a long-period transparent bioactive material for corneal repair in the process of corneal tissue renovation. This article discusses the silk fibroin and formamide blend membranes as a corneal stroma repair material. Silk fibroin solution was mixed with formamide in different proportions to obtain insoluble transparent silk fibroin film by casting method. The blending membranes had excellent mechanical properties, cell compatibility and long-term transparent properties. Rabbit corneal stromal cells were seeded on the sterilized composite films. The rate of cell surface adhesion was over 90% after cells were placed on it for 5 hours. When cells were seeded on blend membranes from one day to seven days, Alma Blue was added to complete medium. Compared with the cell culture plate, there was no significant difference in cell proliferation on formamide/silk films. The results indicated that formamide/silk films might be used as a corneal stroma repair material and worth of further investigation.

Citation： ZHANGShanshan, LIJiaojiao, ZHANGFang, ZHANGXiaofeng, LUShenzhou. Development of Silk Fibroin Composite Films for Corneal Repair. Journal of Biomedical Engineering, 2015, 32(4): 867-873,886. doi: 10.7507/1001-5515.20150155 Copy

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1. 宋秀君.重视角膜基质细胞的作用[J].中华实验眼科杂志,2011,29(12):1057-1060.
2. HORI J,JOYCE N,STREILEIN J W.Epithelium-deficient corneal allografts display immune privilege beneath the kidney capsule[J].Invest Ophthalmol Vis Sci,2000,41(2):443-452.
3. BOULET-AUDET M,TERRY A E,VOLLRATH F,et al.Corrigendum to "Silk protein aggregation kinetics revealed by Rheo-IR"[J].Acta Biomater,2014,10(6):776-784.
4. BRAY L J,GEORGE K A,AINSCOUGH S L,et al.Human corneal epithelial equivalents constructed on Bombyx mori silk fibroin membranes[J].Biomaterials,2011,32(22):5086-5091.
5. LAWRENCE B D,MARCHANT J K,PINDRUS M A,et al.Silk film biomaterials for cornea tissue engineering[J].Biomaterials,2009,30(7):1299-1308.
6. KWEON H,HA H C,UM I C,et al.Physical properties of silk fibroin/chitosan blend films[J].J Appl Polym Sci,2001,80(7):928-934.
7. GIL E S,MANDAL B B,PARK S H,et al.Helicoidal multi-lamellar features of RGD-functionalized silk biomaterials for corneal tissue engineering[J].Biomaterials,2010,31(34):8953-8963.
8. 张晓峰,刘铁连,杨吉成,等.物理交联再生丝素膜组织工程角膜的生物相容性研究[J].中华实验眼科杂志,2011,29(9):780-785.
9. 罗敏清,张岑岑,毛丽,等.水不溶性透明丝素蛋白膜的制备及性能[J].高分子材料科学与工程,2011,27(8):141-145.
10. 位晓娟,刘万顺,韩宝芹,等.兔角膜内皮细胞载体的体外培养及移植的研究[J].中国生物医学工程学报,2006,25(5):590-595.
11. ARAI T,FREDDI G,INNOCENTI R,et al.Biodegradation of Bombyx mori silk fibroin fibers and films[J]. J Appl Polym Sci, 2004, 91(4):2383-2390.
12. 陈义,侯光辉,吴静,等.培养兔角膜基质细胞的一种新方法——悬浮基质片法[J].眼科新进展, 2010, 30(2):124-127.
13. O'BRIEN J, WILSON I, ORTON T, et al. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity[J]. Eur J Biochem, 2000, 267(17):5421-5426.
14. ANOOPKUMAR-DUKIE S, CAREY J B, CONERE T, et al. Resazurin assay of radiation response in cultured cells[J]. Br J Radiol, 2005, 78(934):945-947.
15. OKUSHITA K, ASANO A, WILLIAMSON M P, et al. Local structure and dynamics of serine in the heterogeneous structure of the crystalline domain of Bombyx mori silk fibroin in silk Ⅱ form studied by 2D 13C-13C homonuclear correlation NMR and relaxation time observation[J]. Macromolecules, 2014, 47(13):4308-4316.
16. LOTZ B, KEITH H D. Crystal structure of poly(L-Ala-Gly)Ⅱ. A model for silk. Ⅰ[J]. J Mol Biol, 1971, 61(1):201-215.
17. ISHIKAWA H, NAGURA M. Structure and physical properties of silk fibroin[J]. Sen-i Gakkaishi, 1983, 39(10):353-363.
18. ASAKURA T, KUZUHARA A, TABETA R, et al. Conformational characterization of Bombyx mori silk fibroin in the solid state by high-frequency carbon-13 cross polarization-magic angle spinning NMR,X-ray diffraction,and infrared spectroscopy[J]. Macromolecules, 1985, 18(10):1841-1845.
19. NUMATA K, CEBE P, KAPLAN D L. Mechanism of enzymatic degradation of beta-sheet crystals[J]. Biomaterials, 2010, 31(10):2926-2933.
20. LI M Z, LU S Z, WU Z Y. Study on porous silk filbroin materials.Ⅰ. fine structure of freeze-dried silk fibroin[J]. J Appl Polym Sci, 2001, 79:2185-2191.
21. SHANG Songmin, ZHU Lei, FAN Jintu. Physical properties of silk fibroin/cellulose blend films regenerated from the hydrophilic Ionic liquid[J]. Carbohydr Polym, 2011, 86(2):462-468.
22. KWEON H Y, PARK Y H. Structural and conformational changes of regenerated Antheraea pernyi silk fibroin films treated with methanol solution[J]. J Appl Polym Sci, 1999, 73(14):2887-2894.
23. 徐水,张胡静,李雯静,等.丝素/纳米SiO2凝胶共混膜的制备及性能测试[J].蚕业科学, 2011, 37(1):82-87.
24. DOUTCH J, QUANTOCK A J, SMITH V A, et al. Light transmission in the human cornea as a function of position across the ocular surface:theoretical and experimental aspects[J]. Biophys J, 2008, 95(11):5092-5099.
25. 李纲,钱婷婷,洪佳旭,等.丝素蛋白和乳酸-己内酯共聚物纳米纤维支架构建组织工程化角膜上皮的实验研究[J].中国眼耳鼻喉科杂志, 2013, 13(2):96-99, 104.
26. ZENG Yanjun, YANG Jian, HUANG Kun, et al. A comparison of biomechanical properties between human and porcine cornea[J]. J Biomech, 2001, 34(4):533-537.

Journal of Biomedical Engineering

Development of Silk Fibroin Composite Films for Corneal Repair

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