Expression of Urokinase-type Plasminogenactivator and Phosphorylation of Glycogen Synthase Kinase-3β in Colorectal Adenocarcinoma and Its Significance_West China Medical Journal

Authors：

 ZHOUShi-guang ¹ ,  YANGJian-hua ² , WANGYing-kun ³ , FENGLing-xiang ²

1. Department of Pathology, China Minmetal Hanxing Staff General Hospital, Handan, Hebei 056001, P. R. China;
2. Department Pathology, Handan Central Hospital, Handan, Hebei 056001, P. R. China;
3. Department Pathology, Ningjin County Maternal and Child Health Hospital, Ningjin, Hebei 055550, P. R. China;

Corresponding author：

YANGJian-hua, Email: yangjianhua301@163.com

Keywords：

Colorectal adenocarcinoma; Urokinase-type plasminogenactivator; Phosphorylation of glycogen synthase kinase-3β; Immunohistochemistry

DOI：

10.7507/1002-0179.20140011

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Abstract Full text Figures/Tables Video References Cited by

Objective To study the expression of urokinase-type plasminogenactivator (uPA) and phosphorylation of glycogen synthase kinase-3β (P-GSK3β) in human colorectal adenocarcinoma and its significance. Methods Seventy-eight samples of colorectal adenocarcinoma got during operation between January 2006 and December 2010 in Handan Central Hospital were chosen as the study subjects. The immunohistochemical SP method was used to detect uPA and P-GSK3β levels in the 78 cases of colorectal adenocarcinoma, 20 cases of normal colorectal mucosa and 30 cases of colorectal adenoma. Results The positive expression rates of uPA and P-GSK3β in colorectal carcinoma were much higher than those in colorectal mucosa, colorectal polyps, and colorectal adenoma (P<0.05). The expressions of uPA and P-GSK3β were closely correlated with the differentiation, TNM and lymph nodes metastasis (P<0.05). Conclusion The expression of uPA and P-GSK3β is closely related to the colorectal adenocarcinoma occurrence. Both of them are important biological markers in colorectal adenocarcinoma occurrence and development.

Citation： ZHOUShi-guang, YANGJian-hua, WANGYing-kun, FENGLing-xiang. Expression of Urokinase-type Plasminogenactivator and Phosphorylation of Glycogen Synthase Kinase-3β in Colorectal Adenocarcinoma and Its Significance. West China Medical Journal, 2014, 29(1): 39-42. doi: 10.7507/1002-0179.20140011 Copy

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1. 朱光君, 熊正文, 苏红, 等. 标准型CD44基因蛋白在78例非小细胞肺癌中表达的定量研究[J]. 中国组织化学与细胞化学杂志, 2002, 11(2):174-177.
2. 熊正文, 王炳胜, 朱光君, 等. CD34, CD31与FVⅢ显示非小细胞肺癌微血管密度的对比研究[J].中国医师杂志, 2003, 5(4):438-441.
3. Parenti A, Leo G, Porzionato A, et al. Expression of survivin, p53, and caspase 3 in Barrett's esophagus carcinogenesis[J]. Hum Pathol, 2006, 37(1):16-22.
4. Tan X, Egami H, Nozawa F, et al. Analysis of the invasion-metastasis mechanism in pancreatic cancer:involvement of plasminogen cascade proteins in the invasion of pancreatic cancer cells[J]. Int J Oncol, 2006, 28(2):369-374.
5. 丁友成, 朱正纲, 刘炳亚, 等. 胃癌细胞uPA表达与腹膜种植潜能的相关性研究[J]. 中华肿瘤杂志, 2005, 27(1):16-18.
6. Hildenbrand R, Gandhari M, Stroebel P, et al. The urokinase-system-role of cell proliferation and apoptosis[J]. Histol Histopathol, 2008, 23(2):227-236.
7. Pedersen AN, Mouridsen HT, Tenney DY, et al. Immunoassays of urokinase (uPA) and its type-1 inhibitor (PAI-1) indetergent extracts of breast cancer tissue[J]. Eur J Cancer, 2003, 39(7):899-908.
8. 王艳丽, 高青, 谢霞. 糖原合酶激酶-3β对胃癌细胞生长的影响及其机制[J].肿瘤防治研究, 2009, 36(8):647-650.
9. Quintayo MA, Munro AF, Thomas J, et al. GSK3β and cyclin D1 expression predicts outcome in early breast cancer patients[J]. Breast Cancer Res Treatment, 2012, 136(1):161-168.
10. Song R, Wang X, Mao Y, et al. Resistin disrupts glycogen synthesis under high insulin and high glucose levels by down-regulating the hepatic levels of GSK3β[J]. Gene, 2013, 529(1):50-56.
11. 汪华, 邓长生, 余峰. 糖原合成酶激酶-3β与胃癌细胞增殖关系的研究[J]. 数理医药学杂志, 2006, 19(5):509-510.
12. Ma CY, Ji WT, Chueh FS, et al. Butein inhibits the migration and invasion of SK-HEP-1 human hepatocarcinoma cells through suppressing the ERK, JNK, p38, and uPA signaling multiple pathways[J]. J Agricul Food Chem, 2011, 59(16):9032-9038.

West China Medical Journal

Expression of Urokinase-type Plasminogenactivator and Phosphorylation of Glycogen Synthase Kinase-3β in Colorectal Adenocarcinoma and Its Significance

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