Comparison and Evaluation of Diagnostic Performance of Four Different Methods for Detection of Anti-dsDNA Antibodies_West China Medical Journal

Authors：

 YINGJun , ZHAOJing

Laboratory of Santai People's Hospital, Santai, Sichaun 621100, P. R. China;

Corresponding author：

Keywords：

Anti-dsDNA antibody; Systemic lupus erythematosus; Crithidia luciliae immunofluorescence test; Enzyme linked immunosorbent assay; Linear immunoassay; Chemiluminescence immunoassay

DOI：

10.7507/1002-0179.20140398

Video：

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Objective To evaluate diagnostic performance of crithidia luciliae immunofluorescence test (CLIFT), enzyme linked immunosorbent assay (ELISA), linear immunoassay (LIA) and chemiluminescence immunoassay (CLIA) for detection of anti-dsDNA antibodies for systemic lupus erythematosus (SLE). Methods A total of 178 sera[SLE (n=86), other systemic rheumatic diseases (n=62), and healthy individual (n=30)], from whom received treatment from July 2012 to June 2013, were tested by 4 different assay kits. Results The diagnostic performances of four methods for detecting anti-dsDNA antibodies for SLE were ELISA, CLIA, CLIFT and LIA, from higher to lower; while ELISA had the highest sensitivity (67.4%), and CLIA had the highest specificity (95.6%). The three test methods (ELISA, LIA, CLIA) had almost perfect concordance with the comparison method (CLIFT, Kappa >0.8). With cut-off values set at 95% of specificity, there was no statistical difference of sensitivity between ELISA and CLIA (58.1%, 60.5%; P>0.05). Conclusion Four assays can be used for the clinical detection of anti-dsDNA antibodies, and the results have an almost perfect concordance. Different assays show various performances depending on the methods and cut-off values used.

Citation： YINGJun, ZHAOJing. Comparison and Evaluation of Diagnostic Performance of Four Different Methods for Detection of Anti-dsDNA Antibodies. West China Medical Journal, 2014, 29(7): 1305-1308. doi: 10.7507/1002-0179.20140398 Copy

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5.	Herbst R, Liu Z, Jallal B, et al. Biomarkers for systemic lupus erythematosus[J]. Int J Rheum Dis, 2012, 15(5):433-444.
6.	Adelowo OO, Ojo O, Oduenyi I. Auto antibodies in Nigerian lupus patients[J]. Afr J Med Med Sci, 2012, 41(2):177-181.
7.	Liu CC, Manzi S, Ahearn JM. Biomarkers for systemic lupus erythematosus:a review and perspective[J]. Curr Opin Rheumatol, 2005, 17(5):543-549.
8.	Zigon P, Lakota K, Cucnik S, et al. Comparison and evaluation of different methodologies and tests for detection of anti-dsDNA antibodies on 889 Slovenian patients' and blood donors' sera[J]. Croat Med J, 2011, 52(6):694-702.
9.	Egner W. The use of laboratory tests in the diagnosis of SLE[J]. J Clin Pathol, 2000, 53(6):424-432.
10.	Antico A, Platzgummer S, Bassetti D, et al. Diagnosing systemic lupus erythematosus:new-generation immunoassays for measurement of anti-dsDNA antibodies are an effective alternative to the Farr technique and the Crithidia luciliae immunofluorescence test[J]. Lupus, 2010, 19(8):906-912.
11.	Kim KH, Han JY, Kim JM, et al. Clinical significance of ELISA positive and immunofluorescence negative anti-dsDNA antibody[J]. Clin Chim Acta, 2007, 380(1/2):182-185.
12.	K andala NB, Connock M, G rove A, et al. Belimumab:a technological advance for systemic lupus erythematosus patients? Report of a systematic review and meta-analysis[J]. BMJ Open, 2013, 3(7):e002852.
13.	Rekvin OP. Anti-dsDNA antibodies as a classification criterion and a diagnostic marker for SLE:Critical remarks[J/OL]. Clin Exp Immunol, 2014. http://dx.doi.org/10.1111/cei.12296.
14.	Janyapoon K, Jivakanont P, Choosang K, et al. Comparative study of anti-double stranded DNA detection by ELISA and Crithidia luciliae immunofluorescence[J]. Southeast Asian J Trop Med Public Health, 2003, 34(3):646-650.
15.	Yang JY, Oh EJ, Kim Y, et al. Evaluation of Anti-dsDNA antibody tests:crithidia luciliae immunofluorescence test, immunoblot, enzyme-linked immunosorbent assay, chemiluminescence immunoassay[J]. Korean J Lab Med, 2010, 30(6):675-684.

1. Lewis RM, Stollar BD, Goldberg EB. A rapid, sensitive test for the detection of antibodies to DNA[J]. J Immunol Methods, 1973, 3(4):365-374.
2. Manson JJ, Ma A, Rogers P, et al. Relationship between anti-dsDNA, anti-nucleosome and anti-alpha-actinin antibodies and markers of renal disease in patients with lupus nephritis:a prospective longitudinal study[J]. Arthritis Res Ther, 2009, 11(5):R154.
3. 中华医学会风湿病学分会. 系统性红斑狼疮诊断及治疗指南[J]. 中华风湿病学杂志, 2010, 14(5):342-346.
4. Liu CC, Kao AH, Manzi S, et al. Biomarkers in systemic lupus erythematosus:challenges and prospects for the future[J]. Ther Adv Musculoskelet Dis, 2013, 5(4):210-233.
5. Herbst R, Liu Z, Jallal B, et al. Biomarkers for systemic lupus erythematosus[J]. Int J Rheum Dis, 2012, 15(5):433-444.
6. Adelowo OO, Ojo O, Oduenyi I. Auto antibodies in Nigerian lupus patients[J]. Afr J Med Med Sci, 2012, 41(2):177-181.
7. Liu CC, Manzi S, Ahearn JM. Biomarkers for systemic lupus erythematosus:a review and perspective[J]. Curr Opin Rheumatol, 2005, 17(5):543-549.
8. Zigon P, Lakota K, Cucnik S, et al. Comparison and evaluation of different methodologies and tests for detection of anti-dsDNA antibodies on 889 Slovenian patients' and blood donors' sera[J]. Croat Med J, 2011, 52(6):694-702.
9. Egner W. The use of laboratory tests in the diagnosis of SLE[J]. J Clin Pathol, 2000, 53(6):424-432.
10. Antico A, Platzgummer S, Bassetti D, et al. Diagnosing systemic lupus erythematosus:new-generation immunoassays for measurement of anti-dsDNA antibodies are an effective alternative to the Farr technique and the Crithidia luciliae immunofluorescence test[J]. Lupus, 2010, 19(8):906-912.
11. Kim KH, Han JY, Kim JM, et al. Clinical significance of ELISA positive and immunofluorescence negative anti-dsDNA antibody[J]. Clin Chim Acta, 2007, 380(1/2):182-185.
12. K andala NB, Connock M, G rove A, et al. Belimumab:a technological advance for systemic lupus erythematosus patients? Report of a systematic review and meta-analysis[J]. BMJ Open, 2013, 3(7):e002852.
13. Rekvin OP. Anti-dsDNA antibodies as a classification criterion and a diagnostic marker for SLE:Critical remarks[J/OL]. Clin Exp Immunol, 2014. http://dx.doi.org/10.1111/cei.12296.
14. Janyapoon K, Jivakanont P, Choosang K, et al. Comparative study of anti-double stranded DNA detection by ELISA and Crithidia luciliae immunofluorescence[J]. Southeast Asian J Trop Med Public Health, 2003, 34(3):646-650.
15. Yang JY, Oh EJ, Kim Y, et al. Evaluation of Anti-dsDNA antibody tests:crithidia luciliae immunofluorescence test, immunoblot, enzyme-linked immunosorbent assay, chemiluminescence immunoassay[J]. Korean J Lab Med, 2010, 30(6):675-684.

West China Medical Journal

Comparison and Evaluation of Diagnostic Performance of Four Different Methods for Detection of Anti-dsDNA Antibodies

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