Analysis of the Relationship between Fluorescence Patterns of Antinuclear Antibody and Antinuclear Antibody Profiles_West China Medical Journal

Authors：

 WANGLi ¹ , RENLi-ling ² , GAOYu ¹ , LIWen ¹ , LIShuo ³ ,  WUYong-kang ⁴

1. Department of Gynecology and Obstetrics, 452 Hospital of PLA, Chengdu, Sichuan 610061, P. R. China;
2. Department of Geriatrics Neurology, the Fifth Hospital of Chengdu, Chengdu, Sichuan 611130, P. R. China;
3. Department of Nutrition, 452 Hospital of PLA, Chengdu, Sichuan 610061, P. R. China;
4. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, P. R. China;

Corresponding author：

WUYong-kang, Email: vipwyk@163.com

Keywords：

Antibodies; antinuclear; Antinuclear antibody profiles; Autoantibody; Comparative study

DOI：

10.7507/1002-0179.20150023

Video：

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Objective To discuss the relationship between antinuclear antibody (ANA) fluorescence pattern detected by indirect immunity fluorescence (IIF) and antinuclear antibody profiles (including anti-dsDNA, RNP, Sm, SSa, SSb, Scl-70, Jo-1 and rib-P) in human serum. Methods A total of 7385 cases of ANA pattern and ANA profiles were retrospectively analyzed from January 2010 to December 2013. ANA was detected by IIF substrated as HEp-2 cells, anti-dsDNA by IIF substrated as crithidia, and the other 7 antibodies by enzyme immunoblot with purified antigen. Results Granular pattern mostly presented as anti-RNP, anti-Sm, anti-SSa and anti-SSb (P < 0.001); homogeneous pattern was anti-dsDNA and anti-SSa (P < 0.001); nucleolus, centromere, and mixed pattern was anti-SSa (P < 0.05); cytoplasm pattern was anti-rib-P and anti-SSa (P < 0.05). But few above antibodies could be detected in Golgi, dots, rim, actin, actotropomyosin, prolifevating cell nuclear antigen (PCNA) and vementin pattern. Homogeneous pattern was shown up to 77.91% in only anti-dsDNA positive serum; granular was 96.84%, 52.01%, and 82.35% respectively in only anti-RNP or anti-SSa or anti-Sm positive. Homogeneous and nucleolus mix pattern was up to 30.53% in only anti-Scl-70 positive. Cytoplasm pattern was 50.00% and 61.54% respectively in only anti-rib-P or anti-Jo-1 positive. But no fixed relationship was found between ANA pattern and anti-SSb. Conclusions There is a certain relationship between ANA and antinuclear antibody profiles. Granular, homogeneous and cytoplasm pattern often can be detected more than one autoantibodies. Eight kinds of specific autoantibodies often are negative when ANA patterns are centromere, Golgi, dots, rim, actin, tropomyosin, PCNA, and vimentin. Anti-dsDNA is mainly corresponding to homogeneous, anti-RNP, anti-SSa and anti-Sm to granular, anti-Scl-70 to homogeneous and nucleoli, anti-rib-P and anti-Jo-1 to cytoplasm. The study can give suggestions for further tests application and lab result checking.

Citation： WANGLi, RENLi-ling, GAOYu, LIWen, LIShuo, WUYong-kang. Analysis of the Relationship between Fluorescence Patterns of Antinuclear Antibody and Antinuclear Antibody Profiles. West China Medical Journal, 2015, 30(1): 84-88. doi: 10.7507/1002-0179.20150023 Copy

1.	Thomas L.临床实验诊断学:实验结果的应用和评估[M].朱汉民, 沈霞, 吕元, 等, 译.上海:上海科学技术出版社, 2004:794.
2.	郭春, 谭立明, 李洪文, 等. ENA检测对诊断系统性红斑狼疮的临床应用[J].中国实用医药, 2007, 2(31):118-119.
3.	彭晓东, 王兰兰, 张瑞薇, 等.抗核抗体荧光免疫检测质量控制方法的建立和应用[J].中华检验医学杂志, 2004, 27(3):190-192.
4.	Kidd K, Cusi K, Mueller R, et al. Detection and identification of significant ANAs in previously determined ANA negative samples[J]. Clin Lab, 2005, 51(9/10):517-521.
5.	王莉, 任丽玲, 高宇, 等.抗Ro52抗体和抗Ro60抗体的临床应用价值探讨[J].华西医学, 2014, 29(5):879-882.
6.	Tani C, Carli L, Vagnani S, et al. The diagnosis and classification of mixed connective tissue disease[J]. J Autoimmun, 2014(48/49):46-49.
7.	Wu Y, Cai B, Feng W, et al. Double positive CD4+CD8+T cells:key suppressive role in the production of autoantibodies in systemic lupus erythematosus[J]. Indian J Med Res, 2014, 140(4):513-519.
8.	Ter Borgl EJ, Kelder JC. Lower prevalence of extra-glandular manifestations and anti-SSB antibodies in patients with primary Sjogren's syndrome and widespread pain:evidence for a relatively benign subset[J]. Clin Exp Rheumatol, 2014, 32(3):349-353.
9.	Adelowo OO, Umeizudike T, Olaosebikan H, et al. Nephritis as an initial diagnosis of lupus in Nigerian patients[J]. Afr J Med Med Sci, 2014, 43(2):99-105.
10.	Pasoto SG, Trindade Viana VS, Bonfa E. The clinical utility of anti-ribosomal P autoantibodies in systemic lupus erythematosus[J]. Expert Rev Clin Immunol, 2014, 10(11):1493-1503.
11.	罗松, 梁前进, 王春梅, 等.重组表达的着丝粒蛋白-B N-端抗原在抗着丝粒自身免疫病血清检测中的应用[J].解剖学报, 2004, 35(1):35-38.
12.	武永康, 王兰兰, 彭晓东, 等.抗着丝点抗体在自身免疫性疾病中的临床应用价值探讨[J].华西医学, 2005, 20(4):650-652.
13.	Tonutti E, Bassetti D, Piazza A, et al. Diagnostic accuracy of Elisa methods as an alternative screening test to indirect immunofluorescence for the detection of antinuclear antibodies. Evaluation of five commercial kits[J]. Autoimmunity, 2004, 37(2):171-176.
14.	Almeida Gonzalez D, Casanas Rodriguez C, Magdalena Armas L, et al. Anti-ENA profiles related with anti-SS-A/Ro. The detection of Ro52 and Ro60 according to the presence of SS-B/La, and ANA pattern and titer[J]. Immunol Lett, 2014, 161(1):6-12.
15.	Li WX, Pan HF, Li LH, et al. Prevalence and clinical significance of 15 autoantibodies in patients with new-onset systemic lupus erythematosus[J]. Ir J Med Sci, 2010, 179(4):623-627.
16.	Hamaguchi Y, Fujimoto M, Matsushita T, et al. Common and distinct clinical features in adult patients with anti-aminoacyl-tRNA synthetase antibodies:heterogeneity within the syndrome[J]. PLoS One, 2013, 8(4):e60442.

1. Thomas L.临床实验诊断学:实验结果的应用和评估[M].朱汉民, 沈霞, 吕元, 等, 译.上海:上海科学技术出版社, 2004:794.
2. 郭春, 谭立明, 李洪文, 等. ENA检测对诊断系统性红斑狼疮的临床应用[J].中国实用医药, 2007, 2(31):118-119.
3. 彭晓东, 王兰兰, 张瑞薇, 等.抗核抗体荧光免疫检测质量控制方法的建立和应用[J].中华检验医学杂志, 2004, 27(3):190-192.
4. Kidd K, Cusi K, Mueller R, et al. Detection and identification of significant ANAs in previously determined ANA negative samples[J]. Clin Lab, 2005, 51(9/10):517-521.
5. 王莉, 任丽玲, 高宇, 等.抗Ro52抗体和抗Ro60抗体的临床应用价值探讨[J].华西医学, 2014, 29(5):879-882.
6. Tani C, Carli L, Vagnani S, et al. The diagnosis and classification of mixed connective tissue disease[J]. J Autoimmun, 2014(48/49):46-49.
7. Wu Y, Cai B, Feng W, et al. Double positive CD4+CD8+T cells:key suppressive role in the production of autoantibodies in systemic lupus erythematosus[J]. Indian J Med Res, 2014, 140(4):513-519.
8. Ter Borgl EJ, Kelder JC. Lower prevalence of extra-glandular manifestations and anti-SSB antibodies in patients with primary Sjogren's syndrome and widespread pain:evidence for a relatively benign subset[J]. Clin Exp Rheumatol, 2014, 32(3):349-353.
9. Adelowo OO, Umeizudike T, Olaosebikan H, et al. Nephritis as an initial diagnosis of lupus in Nigerian patients[J]. Afr J Med Med Sci, 2014, 43(2):99-105.
10. Pasoto SG, Trindade Viana VS, Bonfa E. The clinical utility of anti-ribosomal P autoantibodies in systemic lupus erythematosus[J]. Expert Rev Clin Immunol, 2014, 10(11):1493-1503.
11. 罗松, 梁前进, 王春梅, 等.重组表达的着丝粒蛋白-B N-端抗原在抗着丝粒自身免疫病血清检测中的应用[J].解剖学报, 2004, 35(1):35-38.
12. 武永康, 王兰兰, 彭晓东, 等.抗着丝点抗体在自身免疫性疾病中的临床应用价值探讨[J].华西医学, 2005, 20(4):650-652.
13. Tonutti E, Bassetti D, Piazza A, et al. Diagnostic accuracy of Elisa methods as an alternative screening test to indirect immunofluorescence for the detection of antinuclear antibodies. Evaluation of five commercial kits[J]. Autoimmunity, 2004, 37(2):171-176.
14. Almeida Gonzalez D, Casanas Rodriguez C, Magdalena Armas L, et al. Anti-ENA profiles related with anti-SS-A/Ro. The detection of Ro52 and Ro60 according to the presence of SS-B/La, and ANA pattern and titer[J]. Immunol Lett, 2014, 161(1):6-12.
15. Li WX, Pan HF, Li LH, et al. Prevalence and clinical significance of 15 autoantibodies in patients with new-onset systemic lupus erythematosus[J]. Ir J Med Sci, 2010, 179(4):623-627.
16. Hamaguchi Y, Fujimoto M, Matsushita T, et al. Common and distinct clinical features in adult patients with anti-aminoacyl-tRNA synthetase antibodies:heterogeneity within the syndrome[J]. PLoS One, 2013, 8(4):e60442.

West China Medical Journal

Analysis of the Relationship between Fluorescence Patterns of Antinuclear Antibody and Antinuclear Antibody Profiles

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