The Correlation of Amniotic Fluids Levels and Blood Serum Levels of Matrix Metalloproteinases-8 and Interleukin-6 with Preterm Delivery_West China Medical Journal

Authors：

 LIAOXiao-yan ,  CHENYi-fei

Department of Obstetrics and Gynecology, Chengdu Women & Children's Central Hospital, Chengdu, Sichuan 610074, P. R. China;

Corresponding author：

CHENYi-fei, Email: 2601747821@qq.com

Keywords：

Matrix metalloproteinases-8; Interleukin-6; Premature delivery

DOI：

10.7507/1002-0179.20150136

Video：

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Objective To investigate the expression of amniotic fluid levels and blood serum levels of matrix metalloproteinases-8 (MMP-8) and interleukin-6 (IL-6) in women with preterm delivery. Methods Between January 2010 and December 2012, we collected the amniotic fluid of 102 preterm pregnant women and 98 full term pregnant women and analyzed the MMP-8 levels and IL-6 levels in amniotic fluid and blood serum. Meanwhile, we also collected the amniotic fluid to do bacterial culture. Results The amniotic fluid levels of MMP-8 in preterm pregnant women were higher than those in full term pregnant women [(320.45±59.88) vs (153.72±29.12) ng/mL, P<0.05], but there was no obvious discrepancy in the blood serum levels of MMP-8 in the two groups [(9.56±2.11) vs (9.42±2.01) ng/mL, P>0.05]. Both amniotic fluid levels and blood serum levels of IL-6 in preterm pregnant women were significantly higher than the full term pregnant women [(90.5±16.3] vs (20.6±12.5) μg/L, P<0.05; (159.2±20.4) vs (22.3±11.8) μg/L, P<0.05]. The positive bacterial culture rate of preterm pregnant women was higher than the full term pregnant women (8.8% vs 1.0%, P<0.05). Conclusion Infection is the most important reason for preterm pregnancy. MMP-8 level increases in the amniotic fluid, and the level of IL-6 in amniotic fluid and blood serum is a valuable clinical index for identifying premature delivery.

Citation： LIAOXiao-yan, CHENYi-fei. The Correlation of Amniotic Fluids Levels and Blood Serum Levels of Matrix Metalloproteinases-8 and Interleukin-6 with Preterm Delivery. West China Medical Journal, 2015, 30(3): 480-483. doi: 10.7507/1002-0179.20150136 Copy

1.	Blencowe H, Cousens S, Oestergaard MZ, et al. National, regional, and worldwide estimates of preterm birth rates in the year 2010 with time trends since 1990 for selected countries:a systematic analysis and implications[J]. Lancet, 2012, 379(9832):2162-2172.
2.	Ngoc NT, Merialdi M, Abdel-Aleem HA, et al. Causes of stillbirths and early neonatal deaths:data from 7993 pregnancies in six developing countries[J]. Bull World Health Organ, 2006, 84(9):699-705.
3.	Lee J, Kim JS, Park JW, et al. Chronic chorioamnionitis is the most common placental lesion in late preterm birth[J]. Placenta, 2013, 24(8):681-689.
4.	余加林. 产前感染与早产[J]. 实用儿科临床杂志, 2005, 20(12):1169-1171.
5.	Klein LL, Gibbs RS. Infection and preterm birth[J]. Obstet Gynecol Clin North Am, 2005, 32(3):397-410.
6.	Hanemaaijer R, Sorsa T, Kontinen YT, et al. Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells. Regulation by tumor necrosis factor-alpha and doxycycline[J]. Biol Chem, 1997, 272(50):31504-31509.
7.	田春华, 朱甫英, 陶爱群. 宫颈阴道分泌物IL-6、MMP-8检测对早产的诊断价值[J]. 放射免疫学杂志, 2011, 24(1):69-71.
8.	Keelan JA. Pharmacological inhibition of inflammatory pathways for the prevention of preterm birth[J]. J Reprod Immunol, 2011, 88(2):176-184.
9.	Cole AA, Chubinskaya S, Schumacher B, et al. Chondrocyte matrix metalloproteinase-8. Human articular chondrocytes express neutrophil collagenase[J]. J Biol Chem, 1996, 271(18):11023-11026.
10.	Palosaari H, Wahltren J, Larmas M, et al. The expression of MMP-8 in human odontoblasts and dental pulp cells is down-regulated by TGF-beta1[J]. J Dent Res, 2000, 79(1):77-84.
11.	Roh CR, Oh WJ, Yoon Bk, et al. Up-regulation of matrix metalloproteinase-9 in human myometrium during labour:a cytokine-mediated process in uterine smooth muscle cell[J]. Mol Hum Reprod, 2000, 6(1):96-102.
12.	Weiss A, Goldman S, Shalev E. The matrix metalloproteinases (MMPs) in the decidua and fetal membranes[J]. FRONT Biosci, 2007, 12:649-659.
13.	Sennstrom MB, Brauner A, Bystrom B, et al. Matrix metalloproteinase-8 correlates with the cervical ripening process in humans[J]. Acta Obstet Gynecol Scand, 2003, 82(10):904-911.
14.	Rajasekhar L, Liou LB, Chan CY, et al. Matrix metalloproteinase-8 in sera and from polymorphonuclear leucocytes in rheumatoid arthritis:in vitro characterization and correlation with disease activity[J]. Clin Exp Rheumatol, 2004, 22(5):597-602.
15.	Romero R, Avita C, Sathanam V, et al. Amniotic fluid interleukin 6 in preterm labor. Association with infection[J]. J Clin Invest, 1990, 85(5):1392-1400.
16.	Stephen J, Fortunato RP, Menon KF, et al. Inflammatory cytokine (interleukins 1, 6 and 8 and tumor necrosis factor-alpha) release from cultured human fetal membranes in response to endotoxic lipopolysaccharide mirrors amniotic fluid concentrations[J]. Am J Obstet Gynecol, 1996, 174(6):1855-1861.
17.	王丽, 张为远. 母血羊水中IL-6和IL-8水平与绒毛膜羊膜炎关系的研究[J]. 中国实用妇科与产科杂志, 1999, 2(5):35-37.

1. Blencowe H, Cousens S, Oestergaard MZ, et al. National, regional, and worldwide estimates of preterm birth rates in the year 2010 with time trends since 1990 for selected countries:a systematic analysis and implications[J]. Lancet, 2012, 379(9832):2162-2172.
2. Ngoc NT, Merialdi M, Abdel-Aleem HA, et al. Causes of stillbirths and early neonatal deaths:data from 7993 pregnancies in six developing countries[J]. Bull World Health Organ, 2006, 84(9):699-705.
3. Lee J, Kim JS, Park JW, et al. Chronic chorioamnionitis is the most common placental lesion in late preterm birth[J]. Placenta, 2013, 24(8):681-689.
4. 余加林. 产前感染与早产[J]. 实用儿科临床杂志, 2005, 20(12):1169-1171.
5. Klein LL, Gibbs RS. Infection and preterm birth[J]. Obstet Gynecol Clin North Am, 2005, 32(3):397-410.
6. Hanemaaijer R, Sorsa T, Kontinen YT, et al. Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells. Regulation by tumor necrosis factor-alpha and doxycycline[J]. Biol Chem, 1997, 272(50):31504-31509.
7. 田春华, 朱甫英, 陶爱群. 宫颈阴道分泌物IL-6、MMP-8检测对早产的诊断价值[J]. 放射免疫学杂志, 2011, 24(1):69-71.
8. Keelan JA. Pharmacological inhibition of inflammatory pathways for the prevention of preterm birth[J]. J Reprod Immunol, 2011, 88(2):176-184.
9. Cole AA, Chubinskaya S, Schumacher B, et al. Chondrocyte matrix metalloproteinase-8. Human articular chondrocytes express neutrophil collagenase[J]. J Biol Chem, 1996, 271(18):11023-11026.
10. Palosaari H, Wahltren J, Larmas M, et al. The expression of MMP-8 in human odontoblasts and dental pulp cells is down-regulated by TGF-beta1[J]. J Dent Res, 2000, 79(1):77-84.
11. Roh CR, Oh WJ, Yoon Bk, et al. Up-regulation of matrix metalloproteinase-9 in human myometrium during labour:a cytokine-mediated process in uterine smooth muscle cell[J]. Mol Hum Reprod, 2000, 6(1):96-102.
12. Weiss A, Goldman S, Shalev E. The matrix metalloproteinases (MMPs) in the decidua and fetal membranes[J]. FRONT Biosci, 2007, 12:649-659.
13. Sennstrom MB, Brauner A, Bystrom B, et al. Matrix metalloproteinase-8 correlates with the cervical ripening process in humans[J]. Acta Obstet Gynecol Scand, 2003, 82(10):904-911.
14. Rajasekhar L, Liou LB, Chan CY, et al. Matrix metalloproteinase-8 in sera and from polymorphonuclear leucocytes in rheumatoid arthritis:in vitro characterization and correlation with disease activity[J]. Clin Exp Rheumatol, 2004, 22(5):597-602.
15. Romero R, Avita C, Sathanam V, et al. Amniotic fluid interleukin 6 in preterm labor. Association with infection[J]. J Clin Invest, 1990, 85(5):1392-1400.
16. Stephen J, Fortunato RP, Menon KF, et al. Inflammatory cytokine (interleukins 1, 6 and 8 and tumor necrosis factor-alpha) release from cultured human fetal membranes in response to endotoxic lipopolysaccharide mirrors amniotic fluid concentrations[J]. Am J Obstet Gynecol, 1996, 174(6):1855-1861.
17. 王丽, 张为远. 母血羊水中IL-6和IL-8水平与绒毛膜羊膜炎关系的研究[J]. 中国实用妇科与产科杂志, 1999, 2(5):35-37.

West China Medical Journal

The Correlation of Amniotic Fluids Levels and Blood Serum Levels of Matrix Metalloproteinases-8 and Interleukin-6 with Preterm Delivery

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