The Effect of Rotenone on Dopaminic Neurons of Brain in Rat Model of Parkinson's Disease_West China Medical Journal

Authors：

 ZHANGYan-ping , LIYan-gai , XUXiao-chen , WANGYing-jie

Department of Neurologic Medicine, the First People's Hospital of Handan City, Handan, Hebei 056002, P. R. China;

Corresponding author：

Keywords：

Rotenone; Parkinson's disease; Dopamine; Oxidative stress; Malondialdehyde; Glutathione

DOI：

10.7507/1002-0179.20150406

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Objective To study the effect of rotenone on rat substantia nigra dopamine (DA) in the nervous system and oxidative stress parameters (malondialdehyde and glutathione), the influence of rotenone on DA neurons toxic effect and its pathogenesis. Methods This study applied back subcutaneous injection of rotenone in rats [1.0 mg/(kg·d)], and used immunocytochemistry technique to detect changes in the expression of tyrosine kinase (TH) in 10 rats of the control group and 10 rats of the experimental group. Spectrophotometry was used to detect the change of oxidative stress parameters in rats (malondialdehyde and glutathione). Results DA neurons in rats had various degrees of damage. The TH immune response strength of rats in the substantia nigra and striatum decreased significantly. The number of immune response nigra TH positive neurons was significantly less in the experimental group than in the control group (P< 0.01). Spectrophotometer method was used to detect the midbrain nigra of glutathione, which was significantly less in the experimental group than in the control group (P<0.01). Malondialdehyde in the experimental group was significantly higher (P<0.01). Conclusion Rotenone has obvious neurotoxicity, and can lead to the damage of DA neurons and obvious oxidative stress injury in rats, which provides an experimental basis for the pathogenesis of Parkinson's disease, and at the same time provides new targets for the treatment.

Citation： ZHANGYan-ping, LIYan-gai, XUXiao-chen, WANGYing-jie. The Effect of Rotenone on Dopaminic Neurons of Brain in Rat Model of Parkinson's Disease. West China Medical Journal, 2015, 30(8): 1407-1410. doi: 10.7507/1002-0179.20150406 Copy

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6.	石葛明, 樊平, 崔慧先, 等. 帕金森症患者脑标本5羟色胺转运体的表达[J]. 基础医学与临床, 2007, 27(3):263-266.
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9.	Li YQ, Li H, Kaneko T, et al. Morphological features and electrophysiological properties of serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleus. An intracellular recording and labeling study in rat brain slices[J]. Brain Res, 2001, 900(1):110-118.
10.	Mori S, Takino T, Yamada H, et al. Immunohistochemical demonstration of serotonin nerve fibers in the subthalamic nucleus of the rat, cat and monkey[J]. Neurosci Lett, 1985, 62(3):305-309.
11.	Cummings JL, Masterman DL. Depression in patients with Parkinson's disease[J]. Int J Geriatr Psychiatry, 1999, 14(9):711-718.
12.	Heinz A, Ragan P, Jones DW, et al. Reduced central serotonin transporters in alcoholism[J]. Am J Psychiatry, 1998, 155(11):1544-1549.
13.	杨咏梅, 聂亚雄, 唐小卿. 鱼藤酮对多巴胺能神经元的损伤作用及机制[J]. 国际病理科学与临床杂志, 2006, 26(2):145-148.
14.	Dexter DT, Carter CJ, Wells FR, et al. Basal lipid peroxidation in substantia nigra is increased in Parkinson's disease[J]. J Neurochem, 1989, 52(2):381-389.
15.	Gorell JM, Johnson CC, Rybicki BA, et al. The risk of Parkinson's disease with exposure to pesticides, farming, well water, and rural living[J]. Neurology, 1998, 50(5):1346-1350.

1. 褚玉霞, 刘健, 冯洁, 等. 帕金森病大鼠中缝背核5-羟色胺能神经元放电频率和放电形式的变化[J]. 生理学报, 2004, 56(5):597-602.
2. Majczyński H, Maleszak K, Cabaj A, et al. Serotonin-related enhancement of recovery of hind limb motor functions in spinal rats after grafting of embryonic raphe nuclei[J]. J Neurotrauma, 2005, 22(5):590-604.
3. 吴少平. 酪氨酸羟化酶及其活性片段在帕金森病中的作用[D]. 北京:中国人民解放军军事医学科学院, 2006:7-8.
4. Eberle-Wang K, Lucki I, Chesselet MF. A role for the subthalamic nucleus in 5-HT_2C-induced oral dyskinesia[J]. Neuroscience, 1996, 72(1):117-128.
5. Shulman LM, Taback RL, Rabinstein AA, et al. Non-recognition of depression and other non-motor symptoms in Parkinson's disease[J]. Parkinsonism Relat Disord, 2002, 8(3):193-197.
6. 石葛明, 樊平, 崔慧先, 等. 帕金森症患者脑标本5羟色胺转运体的表达[J]. 基础医学与临床, 2007, 27(3):263-266.
7. 黄超培, 赵鹏, 鱼藤酮的神经毒性研究进展[J]. 国外医学·卫生学分册, 2005, 32(6):361-365.
8. Nielsen K, Brask D, Knudsen GM, et al. Immunodetection of the serotonin transporter protein is a more valid marker for serotonergic fibers than serotonin[J]. Synapse, 2006, 59(5):270-276.
9. Li YQ, Li H, Kaneko T, et al. Morphological features and electrophysiological properties of serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleus. An intracellular recording and labeling study in rat brain slices[J]. Brain Res, 2001, 900(1):110-118.
10. Mori S, Takino T, Yamada H, et al. Immunohistochemical demonstration of serotonin nerve fibers in the subthalamic nucleus of the rat, cat and monkey[J]. Neurosci Lett, 1985, 62(3):305-309.
11. Cummings JL, Masterman DL. Depression in patients with Parkinson's disease[J]. Int J Geriatr Psychiatry, 1999, 14(9):711-718.
12. Heinz A, Ragan P, Jones DW, et al. Reduced central serotonin transporters in alcoholism[J]. Am J Psychiatry, 1998, 155(11):1544-1549.
13. 杨咏梅, 聂亚雄, 唐小卿. 鱼藤酮对多巴胺能神经元的损伤作用及机制[J]. 国际病理科学与临床杂志, 2006, 26(2):145-148.
14. Dexter DT, Carter CJ, Wells FR, et al. Basal lipid peroxidation in substantia nigra is increased in Parkinson's disease[J]. J Neurochem, 1989, 52(2):381-389.
15. Gorell JM, Johnson CC, Rybicki BA, et al. The risk of Parkinson's disease with exposure to pesticides, farming, well water, and rural living[J]. Neurology, 1998, 50(5):1346-1350.

West China Medical Journal

The Effect of Rotenone on Dopaminic Neurons of Brain in Rat Model of Parkinson's Disease

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