Interleukin-10 Inhibiting the Activation of Dendritic Cells from Chronic Severe Hepatitis B Patients_West China Medical Journal

Authors：

 LuoNanyou ¹ ,  XiaQingjie ²

1. Department of Clinical Laboratory, Longchang People's Hospital, Longchang, Sichuan 642150, P. R. China;
2. Neurological Diseases Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

XiaQingjie, Email: xiaqj2005@126.com

Keywords：

Hepatitis B; Dendritic cells; Interleukin-10

DOI：

10.7507/1002-0179.20160015

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To study the function of interleukin-10 (IL-10) in inhibiting the activation of dendritic cells (DC) in chronic severe hepatitis B patients. Methods Monocytes were isolated from peripheral blood of 16 chronic severe hepatitis B patients between March and September 2012, by ficoll-hypaque density gradient centrifugation and then cultured with plastic-adherence method. Dendritic cells were induced and proliferated from the monocytes with granulocyte-macrophage colony stimulating factor and interleukin-4 for 8 days. Hepatitis B virus core antigen and IL-10 were used to the DC culture to treat DC. The expression of surface marker on dendritic cells was detected by fluorescence-activated cell sorter. The cytotoxic T lymphocyte activity, as well as the interferon (IFN)-γ, IL-12p70 secretion were observed. Results The ratio of CD83, HLA-DR and CD86 positive cells, the concentration of IFN-γ and IL-12p70, as well as the cytotoxic T lymphocyte activity by dendritic cells were significantly increased in hepatitis B virus core antigen treated group and decreased in the IL-10 treated group compared with that in the control group. Meanwhile, the ratio of CD83, HLA-DR and CD86 positive cells, the concentration of IFN-γ and IL-12p70, as well as the cytotoxic T lymphocyte activity by dendritic cells were significantly decreased in IL-10 pretreated plus Hepatitis B virus core antigen treated group compared with that in the hepatitis B virus core antigen treated group. These results indicated that the hepatitis B virus core antigen could induce dendritic cells activation, and IL-10 could inhibit the activation of dendritic cells, even the Hepatitis B virus core antigen being added afterwards. Conclusion IL-10 can inhibit the activation of dendritic cells, and attenuate the cytotoxicity of autologous lymphocytes induced by DC.

Citation： LuoNanyou, XiaQingjie. Interleukin-10 Inhibiting the Activation of Dendritic Cells from Chronic Severe Hepatitis B Patients. West China Medical Journal, 2016, 31(1): 61-65. doi: 10.7507/1002-0179.20160015 Copy

1.	1 石庆凤, 曾维群, 陈敏, 等. IL-10、TNF-α对慢性乙型肝炎患者树突状细胞的影响[J]. 免疫学杂志, 2009(3): 311-313, 317.
2.	2 Op Den Brouw ML, Binda RS, Van Roosmalen MH, et al. Hepatitis B virus surface antigen impairs myeloid dendritic cell function: a possible immune escape mechanism of hepatitis B virus[J]. Immunology, 2009, 126(2): 280-289.
3.	3 Moore KW, de Waal Malefyt R, Coffman RL, et al. Interleukin-10 and the interleukin-10 receptor[J]. Ann Rev Immunol, 2001, 19: 683-765.
4.	4 Hawrylowicz CM, O’garra A. Potential role of interleukin-10-secreting regulatory T cells in allergy and asthma[J]. Nat Rev Immunol, 2005, 5(4): 271-283.
5.	5 Zampino R, Boemio A, Sagnelli C, et al. Hepatitis B virus burden in developing countries[J]. World J Gastroenterol, 2015, 21(42): 11941-11953.
6.	6 Errington F, Steele L, Prestwich R, et al. Reovirus activates human dendritic cells to promote innate antitumor immunity[J]. J Immunol, 2008, 180(9): 6018-6026.
7.	7 Cools N, Van Tendeloo VF, Smits EL, et al. Immunosuppression induced by immature dendritic cells is mediated by TGF-beta/IL-10 double-positive CD4+ regulatory T cells[J]. J Cell Mol Med, 2008, 12(2): 690-700.
8.	8 Cools N, Ponsaerts P, Van Tendeloo VF, et al. Balancing between immunity and tolerance: an interplay between dendritic cells, regulatory T cells, and effector T cells[J]. J Leukoc Biol, 2007, 82(6): 1365-1374.
9.	9 Wang FS, Xing LH, Liu MX, et al. Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection[J]. World J Gastroenterol, 2001, 7(4): 537-541.
10.	10 Zhang Z, Zou ZS, Fu JL, et al. Severe dendritic cell perturbation is actively involved in the pathogenesis of acute-on-chronic hepatitis B liver failure[J]. J Hepatol, 2008, 49(3): 396-406.
11.	11 Pontrelli P, Cariello M, Rascio F, et al. Thrombin May modulate dendritic cell activation in kidney transplant recipients with delayed graft function[J]. Nephrol Dial Transplant, 2015, 30(9): 1480-1487.
12.	12 Martin C, Espaillat MP, Santiago-Schwarz F. IL-10 restricts dendritic cell (DC) growth at the monocyte-to-monocyte-derived DC interface by disrupting anti-apoptotic and cytoprotective autophagic molecular machinery[J]. Immunol Res, 2015, 63(1): 131-143.
13.	13 Lamhamedi-Cherradi SE, Martin RE, Ito T, et al. Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12[J]. J Immunol, 2008, 180(9): 6000-6009.
14.	14 Chen W, Shi M, Shi F, et al. HBcAg-pulsed dendritic cell vaccine induces Th1 polarization and production of hepatitis B virus-specific cytotoxic T lymphocytes[J]. Hepatol Res, 2009, 39(4): 355-365.
15.	15 Gong Y, Zhao C, Zhao P, et al. Role of IL-10-producing regulatory B Cells in chronic hepatitis B virus infection[J]. Dig Dis Sci, 2015, 60(5): 1308-1314.

1. 1 石庆凤, 曾维群, 陈敏, 等. IL-10、TNF-α对慢性乙型肝炎患者树突状细胞的影响[J]. 免疫学杂志, 2009(3): 311-313, 317.
2. 2 Op Den Brouw ML, Binda RS, Van Roosmalen MH, et al. Hepatitis B virus surface antigen impairs myeloid dendritic cell function: a possible immune escape mechanism of hepatitis B virus[J]. Immunology, 2009, 126(2): 280-289.
3. 3 Moore KW, de Waal Malefyt R, Coffman RL, et al. Interleukin-10 and the interleukin-10 receptor[J]. Ann Rev Immunol, 2001, 19: 683-765.
4. 4 Hawrylowicz CM, O’garra A. Potential role of interleukin-10-secreting regulatory T cells in allergy and asthma[J]. Nat Rev Immunol, 2005, 5(4): 271-283.
5. 5 Zampino R, Boemio A, Sagnelli C, et al. Hepatitis B virus burden in developing countries[J]. World J Gastroenterol, 2015, 21(42): 11941-11953.
6. 6 Errington F, Steele L, Prestwich R, et al. Reovirus activates human dendritic cells to promote innate antitumor immunity[J]. J Immunol, 2008, 180(9): 6018-6026.
7. 7 Cools N, Van Tendeloo VF, Smits EL, et al. Immunosuppression induced by immature dendritic cells is mediated by TGF-beta/IL-10 double-positive CD4+ regulatory T cells[J]. J Cell Mol Med, 2008, 12(2): 690-700.
8. 8 Cools N, Ponsaerts P, Van Tendeloo VF, et al. Balancing between immunity and tolerance: an interplay between dendritic cells, regulatory T cells, and effector T cells[J]. J Leukoc Biol, 2007, 82(6): 1365-1374.
9. 9 Wang FS, Xing LH, Liu MX, et al. Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection[J]. World J Gastroenterol, 2001, 7(4): 537-541.
10. 10 Zhang Z, Zou ZS, Fu JL, et al. Severe dendritic cell perturbation is actively involved in the pathogenesis of acute-on-chronic hepatitis B liver failure[J]. J Hepatol, 2008, 49(3): 396-406.
11. 11 Pontrelli P, Cariello M, Rascio F, et al. Thrombin May modulate dendritic cell activation in kidney transplant recipients with delayed graft function[J]. Nephrol Dial Transplant, 2015, 30(9): 1480-1487.
12. 12 Martin C, Espaillat MP, Santiago-Schwarz F. IL-10 restricts dendritic cell (DC) growth at the monocyte-to-monocyte-derived DC interface by disrupting anti-apoptotic and cytoprotective autophagic molecular machinery[J]. Immunol Res, 2015, 63(1): 131-143.
13. 13 Lamhamedi-Cherradi SE, Martin RE, Ito T, et al. Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12[J]. J Immunol, 2008, 180(9): 6000-6009.
14. 14 Chen W, Shi M, Shi F, et al. HBcAg-pulsed dendritic cell vaccine induces Th1 polarization and production of hepatitis B virus-specific cytotoxic T lymphocytes[J]. Hepatol Res, 2009, 39(4): 355-365.
15. 15 Gong Y, Zhao C, Zhao P, et al. Role of IL-10-producing regulatory B Cells in chronic hepatitis B virus infection[J]. Dig Dis Sci, 2015, 60(5): 1308-1314.

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Interleukin-10 Inhibiting the Activation of Dendritic Cells from Chronic Severe Hepatitis B Patients

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