Clinical Values of Phage Amplified Biologically Assay for Diagnosis of Tuberculosis_West China Medical Journal

Authors：

 YanJiangli ¹ , XuJing ¹ , LeiMin ²

1. Department of Clinical Laboratory, Traditional Hospital of Meishan, Meishan, Sichuan 620010, P. R. China;
2. Department of Infectious Deisease, Traditional Hospital of Meishan, Meishan, Sichuan 620010, P. R. China;

Corresponding author：

Keywords：

Phage amplified biologically assay; Phage; Mycobacterium tuberculosis; Tuberculosis infection; Tuberculosis

DOI：

10.7507/1002-0179.20160074

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Objective To evaluate the clinical values of phage amplified biologically assay (PhaB) for diagnosis of tuberculosis by comparatively analyzing the diagnostic performances of PhaB, acid fast stain and culture. Methods The samples of random sputum and morning sputum from 157 tuberculosis patients diagnosed between January and December 2014 were detected by mycobacteria culture, PhaB, acid fast stain and culture method. The differences of diagnostic performances were analyzed by chi-square test. Results The diagnostic sensitivity was 89.8% (mycobacteria culture), 68.2% (PhaB) and 52.2% (acid fast stain); according to the gold standard of culture method, the positive coincident rate was 74.5% and 57.4%, respectively in PhaB and acid fast stain (P<0.05), and the general coincident rate was 75.8% and 60.5% (P<0.05); of those patients with two negative sputum smears, the positive rate was 33.3% (25/75) in PhaB; the detection time was 1 hour (acid fast stain), 46 hours (PhaB) and 9.5 days (mycobacteria culture), respectively. Conclusion Because of its high sensitivity, high specificity and short turn around time, simple operation, distinguishing dead isolates and live isolates and drug resistance detection, PhaB is a new method for screening test of tuberculosis or as an effective complementary testing for traditional assays.

Citation： YanJiangli, XuJing, LeiMin. Clinical Values of Phage Amplified Biologically Assay for Diagnosis of Tuberculosis. West China Medical Journal, 2016, 31(2): 274-277. doi: 10.7507/1002-0179.20160074 Copy

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1. 全国结核病流行病学抽样调查技术指导组. 第四次全国结核病流行病学抽样调查报告[J]. 中华结核和呼吸杂志, 2002, 25(1):6-10.
2. 中华人民共和国卫生部. WS 288-2008 肺结核诊断标准[S]. 人民卫生出版社, 2008.
3. 中华人民共和国卫生部医政司. 全国临床检验操作规程[M]. 3版. 南京:东南大学出版社, 2006.
4. World Health Organization. Global tuberculosis control, epidemiology, stralegy, financing[M]. Geneva:World Health Organization, 2009:411.
5. Diel R, Loddenkemper R, Nienhaus A. Evidence-based comparison of commercial interferon-gamma release assays for detecting active TB:a meta analysis[J]. Chest, 2010, 137(4):952-968.
6. Drobniewski F, Cooke M, Jordan J, et al. Systematic review, meta-analysis and economic modelling of molecular diagnostic tests for antibiotic resistance in tuberculosis[J]. Health Technol Assess, 2015, 19(34):1-188, vii-viii.
7. Slany M. A new cultivation-independent tool for fast and reliable detection of Mycobacterium marinum[J]. J Fish Dis, 2014, 37(4):363-369.
8. Phung TN, Caruso D, Godreuil S, et al. Rapid detection and identification of nontuberculous mycobacterial pathogens in fish by using high-resolution melting analysis[J]. Appl Environ Microbiol, 2013, 79(24):7837-7845.
9. Wilson SM, Al-Suwaidi Z, Mcnerney R, et al. Evaluation of a new rapid bacteriophage-based method for the drug susceptibility testing of Mycobacterium tuberculosis[J]. Nat Med, 1997, 3(4):465-468.
10. 温占波, 李劲松. 分枝杆菌噬菌体在结核病诊断、耐药筛选中的应用研究[J]. 微生物学免疫学进展, 2008, 36(2):60-65.
11. Kiraz N, Et L, Akgun Y, et al. Rapid detection of Mycobacterium tuberculosis from sputum specimens using the FASTPlaqueTB test[J]. Int J Tuberc Lung Dis, 2007, 11(8):904-908.
12. Bonnet M, Gagnidze L, Varaine F, et al. Evaluation of FASTPlaqueTB to diagnose smear-negative tuberculosis in a peripheral clinic in Kenya[J]. Int J Tuberc Lung Dis, 2009, 13(9):1112-1118.
13. Swift BM, Denton EJ, Mahendran SA, et al. Development of a rapid phage-based method for the detection of viable Mycobacterium avium subsp. paratuberculosis in blood within 48 h[J]. J Microbiol Methods, 2013, 94(3):175-179.
14. Albert H, Heydenrych A, Brookes R, et al. Performance of a rapid phage-based test, FASTPlaqueTB, to diagnose pulmonary tuberculosis from sputum specimens in South Africa[J]. Int J Tuberc Lung Dis, 2002, 6(6):529-537.
15. Albay A, Kisa O, Baylan O, et al. The evaluation of FASTPlaqueTB test for the rapid diagnosis of tuberculosis[J]. Diagn Microbiol Infect Dis, 2003, 46(3):211-215.
16. Butt T, Ahmad RN, Afzal RK, et al. Rapid detection of rifampicin susceptibility of Mycobacterium tuberculosis in sputum specimens by mycobacteriophage assay[J]. J Pak Med Assoc, 2004, 54(7):379-382.
17. Watterson SA, Wilson SM, Yates MD, et al. Comparison of three molecular assays for rapid detection of rifampin resistance in Mycobacterium tuberculosis[J]. J Clin Microbiol, 1998, 36(7):1969-1973.
18. Albert H, Heydenrych A, Mole R, et al. Evaluation of FASTPlaqueTB-RIF, a rapid, manual test for the determination of rifampicin resistance from Mycobacterium tuberculosis cultures[J]. Int J Tuberc Lung Dis, 2001, 5(10):906-911.

West China Medical Journal

Clinical Values of Phage Amplified Biologically Assay for Diagnosis of Tuberculosis

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