Expression of yes-associated protein 1 in rats with brain injury_West China Medical Journal

Authors：

 LI Linfang , HU Yingchun , ZHONG Wu , CHEN Muhu

Department of Emergency, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P. R. China;

Corresponding author：

LI Linfang, Email: 472696795@qq.com

Keywords：

Brain injury; Hippo signal pathway; Yes-associated protein 1; Bioinformatics; Rat

DOI：

10.7507/1002-0179.201705079

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the expression of yes-associated protein 1 (YAP1), as a key protein of Hippo signal pathway, in rats with brain injury. Methods A total of 18 Sprague Dawley rats were randomly divided into three groups: normal group, sham operation group and brain injury group. The expression of YAP1 in rats with brain injury was detected by immunochemistry, quantitative polymerase chainreaction and Western blotting. Result Seventy-two hours after the brain injury, the expression level of YAP1 in protein and gene increased significantly in brain injury group, compared with those in the normal and sham operation group (P<0.05). Conclusion The expression of YAP1 increases in rats with brain injury, which maybe a new target for therapy.

Citation： LI Linfang, HU Yingchun, ZHONG Wu, CHEN Muhu. Expression of yes-associated protein 1 in rats with brain injury. West China Medical Journal, 2017, 32(6): 838-841. doi: 10.7507/1002-0179.201705079 Copy

1.	Lu CY, Xia JF, Wang B, et al. Advances in diagnosis, treatments, and molecular mechanistic studies of traumatic brain injury. Biosci Trends, 2015, 9(3): 138-148.
2.	胡迎春, 陈睦虎, 钟武, 等. 大鼠颅脑损伤早期与晚期相关基因生物信息学分析. 医学信息, 2013, 26(18): 269-269, 270.
3.	官明, 陈礼刚, 董劲虎, 等. 半胱氨酸蛋白酶-3 抑制剂 z-DEVD-fmk 对大鼠脑创伤后神经细胞凋亡及血浆血管内皮素、降钙素基因相关肽水平的影响. 临床神经病学杂志, 2014, 27(3): 207-209.
4.	Mukherjee D, Sarmiento JM, Patil CG. Intracranial-pressure monitoring in traumatic brain injury. N Engl J Med, 2013, 368(18): 1748-1749.
5.	Li W, Wang C, Peng J, et al. Naringin inhibits TNF-a induced oxidative stress and inflammatory response in HUVECs via Nox4/NF-κ B and PI3K/Akt pathways. Curr Pharm Biotechnol, 2014, 15(12): 1173-1182.
6.	Zhao J, Xu YM, Zong YJ, et al. Inhibition of Stat3 expression induces apoptosis and suppresses proliferation in human leukemia HL-60 cells. Hematology, 2011, 16(4): 232-235.
7.	Cheng ZG, Zhang GD, Shi PQ, et al. Expression and antioxidation of Nrf2/ARE pathway in traumatic brain injury. Asian Pac J Trop Med, 2013, 6(4): 305-310.
8.	Wu XM, Mao H, Liu J, et al. Dynamic change of SGK expression and its role in neuron apoptosis after traumatic brain injury. Int J Clin Exp Pathol, 2013, 6(7): 1282-1293.
9.	Zhang L, Yan R, Zhang Q, et al. Survivin, a key component of the Wnt/β-catenin signaling pathway, contributes to traumatic brain injury-induced adult neurogenesis in the mouse dentate gyrus. Int J Mol Med, 2013, 32(4): 867-875.
10.	Wierzbicki PM, Rybarczyk A. The hippo pathway in colorectal cancer. Folia Histochem Cytobiol, 2015, 53(2): 105-119.
11.	Maugeri-Saccà M, Barba M, Pizzuti LA, et al. The hippo transducers TAZ and YAP in breast cancer: oncogenic activities and clinical implications. Expert Rev Mol Med, 2015, 17: e14.
12.	Kang W, Cheng AS, Yu J, et al. Emerging role of Hippo pathway in gastric and other gastrointestinal cancers. World J Gastroenterol, 2016, 22(3): 1279-1288.
13.	Harvey KF, Zhang X, Thomas DM. The hippo pathway and human cancer. Nat Rev Cancer, 2013, 13(4): 246-257.
14.	Schlegelmilch K, Mohseni M, Kirak O, et al. Yap1 acts downstream of α-catenin to control epidermal proliferation. Cell, 2011, 144(5): 782-795.
15.	胡迎春, 钟武, 陈睦虎, 等. 基于生物信息学探讨Yes-相关蛋白在大鼠心肌缺血再灌注损伤中的表达. 华西医学, 2016, 31(6): 1008-1012.
16.	Johnson R, Halder G. The two faces of Hippo: targeting the Hippo pathway for regenerative medicine and cancer treatment. Nat Rev Drug Discov, 2014, 13(1): 63-79.
17.	Li N, Lim G, Chen L, et al. Spinal expression of Hippo signaling components YAP and TAZ following peripheral nerve injury in rats. Brain Res, 2013, 1535: 137-147.
18.	Orcholski ME, Zhang Q, Bredesen DE. Signaling via amyloid precursor-like proteins APLP1 and APLP2. J Alzheimers Dis, 2011, 23(4): 689-699.
19.	Heallen T, Zhang M, Wang J, et al. Hippo pathway inhibits Wnt signaling to restrain cardiomyocyte proliferation and heart size. Science, 2011, 332(628): 458-461.
20.	Xin M, Kim Y, Sutherland LB, et al. Hippo pathway effector Yap promotes cardiac regeneration. Proc Natl Acad Sci USA, 2013, 110(34): 13839-13844.
21.	Zhou Q, Li L, Zhao B, et al. The hippo pathway in heart development, regeneration, and diseases. Circ Res, 2015, 116(8): 1431-1447.

1. Lu CY, Xia JF, Wang B, et al. Advances in diagnosis, treatments, and molecular mechanistic studies of traumatic brain injury. Biosci Trends, 2015, 9(3): 138-148.
2. 胡迎春, 陈睦虎, 钟武, 等. 大鼠颅脑损伤早期与晚期相关基因生物信息学分析. 医学信息, 2013, 26(18): 269-269, 270.
3. 官明, 陈礼刚, 董劲虎, 等. 半胱氨酸蛋白酶-3 抑制剂 z-DEVD-fmk 对大鼠脑创伤后神经细胞凋亡及血浆血管内皮素、降钙素基因相关肽水平的影响. 临床神经病学杂志, 2014, 27(3): 207-209.
4. Mukherjee D, Sarmiento JM, Patil CG. Intracranial-pressure monitoring in traumatic brain injury. N Engl J Med, 2013, 368(18): 1748-1749.
5. Li W, Wang C, Peng J, et al. Naringin inhibits TNF-a induced oxidative stress and inflammatory response in HUVECs via Nox4/NF-κ B and PI3K/Akt pathways. Curr Pharm Biotechnol, 2014, 15(12): 1173-1182.
6. Zhao J, Xu YM, Zong YJ, et al. Inhibition of Stat3 expression induces apoptosis and suppresses proliferation in human leukemia HL-60 cells. Hematology, 2011, 16(4): 232-235.
7. Cheng ZG, Zhang GD, Shi PQ, et al. Expression and antioxidation of Nrf2/ARE pathway in traumatic brain injury. Asian Pac J Trop Med, 2013, 6(4): 305-310.
8. Wu XM, Mao H, Liu J, et al. Dynamic change of SGK expression and its role in neuron apoptosis after traumatic brain injury. Int J Clin Exp Pathol, 2013, 6(7): 1282-1293.
9. Zhang L, Yan R, Zhang Q, et al. Survivin, a key component of the Wnt/β-catenin signaling pathway, contributes to traumatic brain injury-induced adult neurogenesis in the mouse dentate gyrus. Int J Mol Med, 2013, 32(4): 867-875.
10. Wierzbicki PM, Rybarczyk A. The hippo pathway in colorectal cancer. Folia Histochem Cytobiol, 2015, 53(2): 105-119.
11. Maugeri-Saccà M, Barba M, Pizzuti LA, et al. The hippo transducers TAZ and YAP in breast cancer: oncogenic activities and clinical implications. Expert Rev Mol Med, 2015, 17: e14.
12. Kang W, Cheng AS, Yu J, et al. Emerging role of Hippo pathway in gastric and other gastrointestinal cancers. World J Gastroenterol, 2016, 22(3): 1279-1288.
13. Harvey KF, Zhang X, Thomas DM. The hippo pathway and human cancer. Nat Rev Cancer, 2013, 13(4): 246-257.
14. Schlegelmilch K, Mohseni M, Kirak O, et al. Yap1 acts downstream of α-catenin to control epidermal proliferation. Cell, 2011, 144(5): 782-795.
15. 胡迎春, 钟武, 陈睦虎, 等. 基于生物信息学探讨Yes-相关蛋白在大鼠心肌缺血再灌注损伤中的表达. 华西医学, 2016, 31(6): 1008-1012.
16. Johnson R, Halder G. The two faces of Hippo: targeting the Hippo pathway for regenerative medicine and cancer treatment. Nat Rev Drug Discov, 2014, 13(1): 63-79.
17. Li N, Lim G, Chen L, et al. Spinal expression of Hippo signaling components YAP and TAZ following peripheral nerve injury in rats. Brain Res, 2013, 1535: 137-147.
18. Orcholski ME, Zhang Q, Bredesen DE. Signaling via amyloid precursor-like proteins APLP1 and APLP2. J Alzheimers Dis, 2011, 23(4): 689-699.
19. Heallen T, Zhang M, Wang J, et al. Hippo pathway inhibits Wnt signaling to restrain cardiomyocyte proliferation and heart size. Science, 2011, 332(628): 458-461.
20. Xin M, Kim Y, Sutherland LB, et al. Hippo pathway effector Yap promotes cardiac regeneration. Proc Natl Acad Sci USA, 2013, 110(34): 13839-13844.
21. Zhou Q, Li L, Zhao B, et al. The hippo pathway in heart development, regeneration, and diseases. Circ Res, 2015, 116(8): 1431-1447.

West China Medical Journal

Expression of yes-associated protein 1 in rats with brain injury

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