• 1. State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an Shaanxi, 710049, P. R. China;
  • 2. Institute for Biomechanics, ETH Zürich, Switzerland;
  • 3. Department of Orthopedics, the First Affiliated Hospital, Xi'an Jiaotong University;
  • 4. Institute of Medical and Biological Engineering, Leeds University, UK;
HEJiankang, Email: jiankanghe@mail.xjtu.edu.cn
Export PDF Favorites Scan Get Citation

Objective To solve the fixation problem between ligament grafts and host bones in ligament reconstruction surgery by using ligament-bone composite scaffolds to repair the ligaments, to explore the fabrication method for ligament-bone composite scaffolds based on three-dimensional (3-D) printing technique, and to investigate their mechanical and biological properties in animal experiments. Methods The model of bone scaffolds was designed using CAD software, and the corresponding negative mould was created by boolean operation. 3-D printing techinique was employed to fabricate resin mold. Ceramic bone scaffolds were obtained by casting the ceramic slurry in the resin mould and sintering the dried ceramics-resin composites. Ligament scaffolds were obtained by weaving degummed silk fibers, and then assembled with bone scaffolds and bone anchors. The resultant ligament-bone composite scaffolds were implanted into 10 porcine left anterior cruciate ligament rupture models at the age of 4 months. Mechanical testing and histological examination were performed at 3 months postoperatively, and natural anterior cruciate ligaments of the right sides served as control. Results Biomechanical testing showed that the natural anterior cruciate ligament of control group can withstand maximum tensile force of (1 384±181) N and dynamic creep of (0.74±0.21) mm, while the regenerated ligament-bone scaffolds of experimental group can withstand maximum tensile force of (370±103) N and dynamic creep of (1.48±0.49) mm, showing significant differences (t=11.617,P=0.000; t=-2.991,P=0.020). In experimental group, histological examination showed that new bone formed in bone scaffolds. A hierarchical transition structure regenerated between ligament-bone scaffolds and the host bones, which was similar to the structural organizations of natural ligament-bone interface. Conclusion Ligament-bone composite scaffolds based on 3-D printing technique facilitates the regeneration of biomimetic ligament-bone interface. It is expected to achieve physical fixation between ligament grafts and host bone.

Citation: ZHANGWenyou, HEJiankang, LIXiang, LIUYaxiong, BIANWeiguo, LIDichen, JINZhongmin. FABRICATION AND IN VIVO IMPLANTATION OF LIGAMENT-BONE COMPOSITE SCAFFOLDS BASED ON THREE-DIMENSIONAL PRINTING TECHNIQUE. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(3): 314-317. doi: 10.7507/1002-1892.20140071 Copy

  • Previous Article

    MECHANICAL PROPERTIES OF POLYLACTIC ACID/β-TRICALCIUM PHOSPHATE COMPOSITE SCAFFOLD WITH DOUBLE CHANNELS BASED ON THREE-DIMENSIONAL PRINTING TECHNIQUE
  • Next Article

    CARTILAGE REPAIR AND SUBCHONDRAL BONE RECONSTRUCTION BASED ON THREE-DIMENSIONAL PRINTING TECHNIQUE