EXPRESSION OF RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-κB LIGAND IN PERIPHERAL BLOOD OF PATIENTS WITH ASEPTIC LOOSENING OF THE IMPLANT AFTER TOTAL HIP APTHROPLASTY_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

HOUKaiyu ,  WANGYufei , LUXiaotao , YANGKemin , KONGWeiyun , LiYonggang

Department of Orthopedics, the Second People's Hospital of Yunnan Province, Kunming Yunnan, 650031, P. R. China;

Corresponding author：

WANGYufei, Email: hhwyf2010@126.com

Keywords：

Total hip arthroplasty; Aseptic loosening; Receptor activator of nuclear factor-κB ligand

DOI：

10.7507/1002-1892.20140188

Video：

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Objective To investigate the expression changes of the receptor activator of nuclear factor-κB ligand (RANKL) in the peripheral blood of patients with aseptic loosening of the implant after total hip arthroplasty (THA) by comparing with that of patients with femoral neck fracture and to analyze the correlation between RANKL expression and aseptic loosening. Methods Between January 2008 and January 2013,the peripheral blood were harvested from 58 patients with aseptic loosening of the implant after THA (trial group) and 63 patients with femoral neck fracture (control group).The 2 groups were well matched,with no significant differences in age and gender (P>0.05).The expressions of the RANKL mRNA and RANKL protein were evaluated by quantitative real-time PCR and Western blot respectively.At the same time,the concentration of RANKL was also measured by ELISA. Results The expression of the RANKL mRNA in the trial group was 18.30±1.09,which was significantly higher than that of control group (1.00±0.05)(t=125.390,P=0.000).The relative RANKL protein expression values in trial group and control group were 0.856±0.254 and 0.404±0.102 respectively,showing significant difference (t=13.032,P=0.000).The results of ELISA showed that the concentration of RANKL in trial group [(3.553 5±0.129 7) ng/mL] was significantly higher than that of control group [(1.912 3±0.126 2) ng/mL] (t=18.124,P=0.000). Conclusion The high RANKL expression in peripheral blood is probably correlated with aseptic loosening of the implant in patients undergoing THA,which possibly is the prognostic factor of aseptic loosening of the implant.

Citation： HOUKaiyu, WANGYufei, LUXiaotao, YANGKemin, KONGWeiyun, LiYonggang. EXPRESSION OF RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-κB LIGAND IN PERIPHERAL BLOOD OF PATIENTS WITH ASEPTIC LOOSENING OF THE IMPLANT AFTER TOTAL HIP APTHROPLASTY. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(7): 853-856. doi: 10.7507/1002-1892.20140188 Copy

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2.	Keener JD,Callaghan JJ,Goetz DD,et al.Twenty-five-year results after Charnley total hip arthroplasty in patients less than fifity years old:a concise follow-up of a previous report.J Bone Joint Surg (Am),2003,85-A(6):1066-1072.
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7.	Qian Y,Zhang XL,Zeng BF,et al.Substance P enhanced titanium particles-induced RANKL expression in fibroblasts from periprosthetic membrane.Connect Tissue Res,2013,54(6):361-366.
8.	奚正德,胡峻熊.RANKL-RANK信号传导与破骨细胞生成及骨病.中国骨质疏松杂志,2008,14(4):285-293.
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14.	Oue E,Lee JW,Sakamoto K,et al.CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction.Biochem Biophys Res Commun,2012,424(3):456-461.
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19.	Theoleyre S,Wittrant Y,Tat SK,et al.The molecular triad OPG/RANK/RANKL:involvement in the orchestration of pathophysiological bone remodeling.Cytokine Growth Factor Rev,2004,15(6):457-475.
20.	张波,郭艾.磨损颗粒引发假体周围骨溶解的机制及药物治疗进展.中国骨质疏松杂志,2007,13(8):596-599.
21.	Kong YY,Yoshida H,Sarosi I,et al.OPGL is a key regulator of osteoclastogenesis,lymphocyte development and lymphnode organogenesis.Nature,1999,397(6717):315-323.
22.	Veigl D,Niederlová J,Krystůfková O.Periprosthethic osteolysis and its association with RANKL expression.Physiol Res,2007,56(4):455-462.
23.	王海,宋科官.磨损颗粒诱导假体周围骨溶解的机制及基因治疗现状.哈尔滨医科大学学报,2011,45(1):95-98.
24.	Kong L,Yang C,Yu L,et al.Pyrroloquinoline quinine inhibits RANKL-mediated expression of NFATc1 in part via suppression of c-Fos in mouse bone marrow cells and inhibits wear particle-induced osteolysis in mice.PLoS One,2013,8(4):e61013.
25.	Chun L,Yoon J,Song Y,et al.The characterization of macrophages and osteoclasts in tissues harvested from revised total hip prostheses.J Biomed Mater Res,1999,48(6):899-903.
26.	耿磊,陈继营,陈城,等.假体周围组织冰冻切片对诊断髋关节假体无菌性松动的特异性分析.中国矫形外科杂志,2012,20(10):909-911.
27.	Alias E,Dharmapatni AS,Holding AC,et al.Polyethylene particles stimulate expression of ITAM-related molecules in peri-implant tissues and when stimulating osteoclastogenesis in vitro.Acta Biomater,2012,8(8):3104-3112.
28.	Granci D,Pellacani A,Spina M,et al.Serum levels of osteoprotegerin and receptor activator of nuclear factor-kappaB ligand as markers of periprosthetic osteolysis.J Bone Joint Surg (Am),2006,88(7):1501-1509.

1. Harris WH.Wear and periprosthetic osteolysis:the problem.Clin Orthop Relat Res,2001,(393):66-70.
2. Keener JD,Callaghan JJ,Goetz DD,et al.Twenty-five-year results after Charnley total hip arthroplasty in patients less than fifity years old:a concise follow-up of a previous report.J Bone Joint Surg (Am),2003,85-A(6):1066-1072.
3. 邵振兴,蒋青.假体周围骨质溶解细胞分子生物学研究.国际骨科学杂志,2007,28(3):170-173.
4. Roato I,Caldo D,D'Amico L,et al.Osteoclastogenesis in peripheral blood mononuclear cell cultures of periprosthetic osteolysis patients and the phenotype of T cells localized in periprosthetic tissues.Biomaterials,2010,31(29):7519-7525.
5. Day JS,Baxter RM,Ramsey ML,et al.Characterization of wear debris in total elbow arthroplasty.J Shoulder Elbow Surg,2013,22(7):924-931.
6. Moon HJ,Yun YP,Han CW,et al.Effect of heparin and alendronate coating on titanium surfaces on inhibition of osteoclast and enhancement of osteoblast function.Biochem Biophys Res Commun,2011,413(2):194-200.
7. Qian Y,Zhang XL,Zeng BF,et al.Substance P enhanced titanium particles-induced RANKL expression in fibroblasts from periprosthetic membrane.Connect Tissue Res,2013,54(6):361-366.
8. 奚正德,胡峻熊.RANKL-RANK信号传导与破骨细胞生成及骨病.中国骨质疏松杂志,2008,14(4):285-293.
9. Lähdeoja T,Pajarinen J,Kouri VP,et al.Toll-like receptors and aseptic loosening of hip endoprosthesis-a potential to respond against danger signals? Orthop Res,2010,28(2):184-190.
10. 吕丹,孙明林.人工髋关节置换后假体无菌性松动的因素及其防治.中国组织工程研究与临床康复,2009,13(13):2553-2556.
11. 李守民,朱晨,孔荣,等.人工髋关节置换术后翻修原因分析及预防.中国骨与关节损伤杂志,2010,25(12):1060-1062.
12. Pajarinen J,Kouri VP,Jämsen E,et al.The response of macrophages to titanium particles is determined by macrophage polarization.Acta Biomater,2013,9(11):9229-9240.
13. Han KH,Ryu JW,Lim KE,et al.Vascular expression of the chemokine CX3CL1 promotes osteoclast recruitment and exacerbates bone resorption in an irradiated murine model.Bone,2014,61(1):91-101.
14. Oue E,Lee JW,Sakamoto K,et al.CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction.Biochem Biophys Res Commun,2012,424(3):456-461.
15. Asagiri M,Takayanagi H.The molecular understanding of osteoclast differentiation.Bone,2007,40(2):251-264.
16. Ryu J,Kim HJ,Chang EJ,et al.Sphingosine 1-phosphate as a regulator of osteoclast differentiation and osteoclast-osteoblast coupling.EMBO J,2006,25(24):5840-5851.
17. Ha J,Lee Y,Kim HH.CXCL2 mediates lipopolysaccharide-induced osteoclastogenesis in RANKLprimed precursors.Cytokine,2011,55(1):48-55.
18. 田虹,樊瑜波.OPG、RANK、RANKL的结构、作用机制和在骨疾病中的作用.现代生物学进展,2010,10(20):3963-3966.
19. Theoleyre S,Wittrant Y,Tat SK,et al.The molecular triad OPG/RANK/RANKL:involvement in the orchestration of pathophysiological bone remodeling.Cytokine Growth Factor Rev,2004,15(6):457-475.
20. 张波,郭艾.磨损颗粒引发假体周围骨溶解的机制及药物治疗进展.中国骨质疏松杂志,2007,13(8):596-599.
21. Kong YY,Yoshida H,Sarosi I,et al.OPGL is a key regulator of osteoclastogenesis,lymphocyte development and lymphnode organogenesis.Nature,1999,397(6717):315-323.
22. Veigl D,Niederlová J,Krystůfková O.Periprosthethic osteolysis and its association with RANKL expression.Physiol Res,2007,56(4):455-462.
23. 王海,宋科官.磨损颗粒诱导假体周围骨溶解的机制及基因治疗现状.哈尔滨医科大学学报,2011,45(1):95-98.
24. Kong L,Yang C,Yu L,et al.Pyrroloquinoline quinine inhibits RANKL-mediated expression of NFATc1 in part via suppression of c-Fos in mouse bone marrow cells and inhibits wear particle-induced osteolysis in mice.PLoS One,2013,8(4):e61013.
25. Chun L,Yoon J,Song Y,et al.The characterization of macrophages and osteoclasts in tissues harvested from revised total hip prostheses.J Biomed Mater Res,1999,48(6):899-903.
26. 耿磊,陈继营,陈城,等.假体周围组织冰冻切片对诊断髋关节假体无菌性松动的特异性分析.中国矫形外科杂志,2012,20(10):909-911.
27. Alias E,Dharmapatni AS,Holding AC,et al.Polyethylene particles stimulate expression of ITAM-related molecules in peri-implant tissues and when stimulating osteoclastogenesis in vitro.Acta Biomater,2012,8(8):3104-3112.
28. Granci D,Pellacani A,Spina M,et al.Serum levels of osteoprotegerin and receptor activator of nuclear factor-kappaB ligand as markers of periprosthetic osteolysis.J Bone Joint Surg (Am),2006,88(7):1501-1509.

Chinese Journal of Reparative and Reconstructive Surgery

EXPRESSION OF RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-κB LIGAND IN PERIPHERAL BLOOD OF PATIENTS WITH ASEPTIC LOOSENING OF THE IMPLANT AFTER TOTAL HIP APTHROPLASTY

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