Vitamin C Promoting Embryonic Stem Cells Co-cultured on Poly 3-Hydroxybutyrate-co-4-Hydroxybutyrate to Differentiating into Myocardiocytes_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

MAYi-xiang ,  MUJun-sheng , ZHANGJian-qun , BOPing

Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P. R. China;

Corresponding author：

MUJun-sheng, Email: wesleymu@hotmail.com

Keywords：

Stem cells; Myocardial patch; Vitamin C; 3-hydroxybutyrate-co-4-hydroxybutyrate scaffold

DOI：

10.7507/1007-4848.20160116

Video：

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Objective To assess the suitability of P (3HB-co-4HB) combined with embryonic stem cells (ESCs) for myocardial patch formation and whether adding vitamin C would improve inductivity or not. Method We extracted mouse embryonic fibrous cell from three clean female white Kunming mouses at a mean body weight of 37.5 grams. We recovered and cultured mouse ESCs. Those mouse embryonic stem cells were obtained from Shanghai Institutes of Biological Sciences. We took pendant-drop method to form embryonic bodies (EBs) and co-cultured them with myocardial patch. The experimental group were cultured in the substate with vitamin C while the control group were cultured in the substate without vitamin C. We immunostained the myocardial patch and observed them by scanning electron microscope. We calculated the differentiation efficiency and mapped the distribution curve of induction time. Results The scattergram showed that the differentiation efficiency increased gradually. The differentiation efficiency of the group with vitamin C was 71.1% and the group without vitamin C was 17.8%. There was a statistical difference between the two groups (P < 0.05). Conclusion On the biological patch of P (3HB-co-4HB), ESCs could grow, proliferate, and differentiate into myocardial cell and adding vitamin C into it could improve the differentiation efficiency.

Citation： MAYi-xiang, MUJun-sheng, ZHANGJian-qun, BOPing. Vitamin C Promoting Embryonic Stem Cells Co-cultured on Poly 3-Hydroxybutyrate-co-4-Hydroxybutyrate to Differentiating into Myocardiocytes. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2016, 23(5): 496-500. doi: 10.7507/1007-4848.20160116 Copy

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1. Mandel Y, Weissman A, Schick R, et al. Human embryonic and induced pluripotent stem cell-derived cardiomyocytes exhibit beat rate variability and power-law behavior. Circulation, 2012, 125(7):883-893.
2. Shiba Y, Fernandes S, Zhu WZ, et al. Human ES-cell-derived cardiomyocytes electrically couple and suppress arrhythmias in injured hearts. Nature, 2012, 489(7415):322-325.
3. Gepstein L, Ding C, Rahmutula D, et al. In vivo assessment of the electrophysiological integration and arrhythmogenic risk of myocardial cell transplantation strategies. Stem Cells, 2010, 28(12):2151-2161.
4. Teng CJ, Jun L, Chiu R, et al. Massive mechanical loss of microsp-heres with direct intramyocardial injection in the beating heart:Implications for cellular cardiomyoplasty. J Thorac Cardiovasc Surg, 2006, 132(3):628-632.
5. Kindi A, Asenjo JF, Ge Y, et al. Microencapsulation to reduce mechanical loss of microspheres:implications in myocardial cell therapy. Eur J Cardiothorac Surg, 2011, 39(2):241-247.
6. Saito T, Kuang JQ, Lin CC, et al. Transcoronary implantation of bone marrow stromal cells ameliorates cardiac function after myoc-ardial infarction. J Thorac Cardiovasc Surg, 2003, 126(1):114-123.
7. Blau HM, Brazelton TR, Weimann JM. The evolving concept of a stem cell:entity or function? Cell, 2001, 105(7):829-841.
8. Orlic D, Hill JM, Arai AE. Stem cells for myocardial regeneration. Circ Res, 2002, 91(12):1092-1102.
9. Roell W, Lewalter T, Sasse P, et al. Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia. Nature, 2007, 450(7171):819-824.
10. Pal R, Mamidi MK, Das AK, et al. Comparative analysis of cardiom-yocyte differentiation from human embryonic stem cells under 3-D and 2-Dculture conditions. J Biosci Bioeng, 2013, 115(2):200-206.
11. Bradley A. Mining the mouse genome. Nature, 2002, 420:512-514.
12. Verma V, Verma P, Ray P, et al. Preparation of scaffolds from human hair proteins for tissue-engineering applications. Biomed Mater, 2008, 3(2):191-196.
13. Nazarov R, Jin HJ, Kaplan DL. Porous 3-D scaffolds from regene-rated silk fibroin. Biomacromolecules, 2004, 5(3):718-726.
14. Tobella LM, Bunster M, Pooley A, et al. Biosynthesis of poly-beta-hydroxyalkanoates by Sphingopyxis chilensis S37 and Wautersia sp. PZK cultured in cellulose pulp mill effluents containing 2, 4, 6-trich-lorophenol. J Ind Microbiol Biotechnol, 2005, 32(9):397-401.
15. Thakor N, Trivedi U, Patel KC. Biosynthesis of medium chain length Poly(3-hydroxyalkanoates) (mcl-PHAs) by Comamonas testosterone during cultivation on vegetable oils. Bioresour Technol, 2005, 96(17):1843-1850.
16. Kose GT, Korkusuz F, Korkusuz P, et al. Bone generation on PHBV matrices:an in vitro study. Biomaterials, 2003, 24(27):4999-5007.
17. Takahashi T, Lord B, Schulze PC, et al. Ascorbic acid enhances differentiation of embryonic stem cells into cardiacmyocytes. Circulation, 2003, 107(14):1912-1916.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Vitamin C Promoting Embryonic Stem Cells Co-cultured on Poly 3-Hydroxybutyrate-co-4-Hydroxybutyrate to Differentiating into Myocardiocytes

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