Synergy of TGF-β1 and TNF-α inducing human bronchial epithelial cells to optimize epithelia-mesenchymal transformation model_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

HE Ming ¹ , ZHU Kai ¹ , DUAN Hongtao ¹ , LI Pengcheng ² , YAN Xiaolong ¹ , ZHANG Zhipei ¹ ,  LI Xiaofei ¹

1. Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, 710038, P.R.China;
2. Department of Respiratory Disease, Tangdu Hospital, Fourth Military Medical University, Xi’an, 710038, P.R.China;

Corresponding author：

LI Xiaofei, Email: lxfchest@fmmu.edu.cn

Keywords：

Transforming growth factor-β1; tumor necrosis factor-α; epithelia-mesenchymal transformation

DOI：

10.7507/1007-4848.201604063

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the effectiveness of the transforming growth factor-β1(TGF-β1) and tumor necrosis factor-α(TNF-α) inducing human bronchial epithelial(HBE) cells to optimize epithelia-mesenchymal transformation(EMT) model. Methods Blank control, TGF-β1 10 ng/ml, TNF-α 10 ng/ml, TGF-β1 10 ng/ml+TNF-α 10 ng/ml induced human epithelial cells for 24 hours. Then the change of morphological alteration were observed by applying CCK8, cells migration assay and Western blot technique. Results When TGF-β1 plus TNF-α induced human epithelial cells for 24 hours, most of HBE cells traits changed including morphological alteration from cobblestone to fusiform, connection between cells vanishing, intercellular space broadening. In the experiments of checking cell migration capacity by the vitro scratch test, the group spacing was 420.06±10.38 μm in the blank control group, 499.86±34.00 μm in the TGF-β1 10 ng/ml group, 514.93±10.56 μm in the TNF-α 10 ng/ml group, 569.68±33.58 μm in the TGF-β1 10 ng/ml+TNF-α10 ng/ml group. TGF-β1 cooperated with TNF-α led to scratch spacing narrowing significantly. Western blot analysis showed that expression of E-cadherin and Vimentin varied significantly in the TGF-β1+TNF-α group. Conclusion Inducing human bronchial epithelial cell by TGF-β1 cooperated with TNF-α optimizes EMT model.

Citation： HE Ming, ZHU Kai, DUAN Hongtao, LI Pengcheng, YAN Xiaolong, ZHANG Zhipei, LI Xiaofei. Synergy of TGF-β1 and TNF-α inducing human bronchial epithelial cells to optimize epithelia-mesenchymal transformation model. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2017, 24(3): 228-232. doi: 10.7507/1007-4848.201604063 Copy

1.	刘星辰, 孙飞, 史宏灿. 排斥反应在同种异体气管移植物中的研究进展. 中国胸心血管外科临床杂志, 2015, 22(1): 71-74.
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6.	Rich JT, Gullane PJ. Current concepts in tracheal reconstruction. Curr Opin Otolaryngol Head Neck Surg, 2012, 20(4): 246-253.
7.	金芬, 张忠寿, 黄卫锋. 上皮间质转化和内皮间质转化在肾纤维化中的研究进展. 海南医学, 2014, 18: 2723-2725.
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13.	Roh YS, Song J, Seki E. TAK1 regulates hepatic cell survival and carcinogenesis. J Gastroenterol, 2014, 49(2): 185-194.
14.	段鸿涛, 刘红刚, 闫小龙, 等. TGF-β/Smad介导上皮细胞间质转化在气管移植后移植体狭窄中的机制研究. 中国胸心血管外科临床杂志, 2016, 23(5): 485-489.
15.	Felton VM1, Inge LJ, Willis BC,et al. Immunosuppression-induced bronchial epithelial-mesenchymal transition: a potential contributor to obliterative bronchiolitis. J Thorac Cardiovasc Surg, 2011, 141(2): 523-530.
16.	Grove DA, Xu J, Joodi R,et al. Attenuation of early airway obstruction by mesenchymal stem cells in a murine model of heterotopic tracheal transplantation. J Heart Lung Transplant, 2011, 30(3): 341-350.
17.	Grove DA, Xu J, Joodi R,et al. TWEAK enhances TGF-beta-induced epithelial-mesenchymal transition in human bronchial epithelial cells. Respir Res, 2015, 16: 48.doi: 10.1186/s12931-015-0207-5.
18.	Ji Q, Liu X, Han Z,et al. Resveratrol suppresses epithelial-to-mesenchymal transition in colorectal cancer through TGF-beta1/Smads signaling pathway mediated Snail/E-cadherin expression. BMC Cancer, 2015, 15: 97. doi: 10.1186/s12885-015-1119-y.
19.	Chen L, Li W, Qiu W,et al. RSRC1 SUMOylation enhances SUMOylation and inhibits transcriptional activity of estrogen receptor beta. FEBS Lett, 2015, 589(13): 1476-1484.
20.	黄颂, 孟爱民. TGF-β调节的EMT在肿瘤浸润、转移中作用的研究进展. 生命科学, 2015, 27(2): 127-134.

1. 刘星辰, 孙飞, 史宏灿. 排斥反应在同种异体气管移植物中的研究进展. 中国胸心血管外科临床杂志, 2015, 22(1): 71-74.
2. Martinod E, Seguin A, Radu DM,et al. Airway transplantation: a challenge for regenerative medicine. Eur J Med Res, 2013, 18: 25.
3. Grillo HC. Bioengineered airway tissue. J Thorac Cardiovasc Surg, 2005, 129, 5: 1208-1209.
4. Tsukada H, Ernst A, Gangadharan S,et al. Tracheal replacement with a silicone-stented, fresh aortic allograft in sheep. Ann Thorac Surg, 2010, 89(1): 253-258.
5. Grillo HC. Tracheal replacement: a critical review. Ann Thorac Surg, 2002, 73(6): 1995-2004.
6. Rich JT, Gullane PJ. Current concepts in tracheal reconstruction. Curr Opin Otolaryngol Head Neck Surg, 2012, 20(4): 246-253.
7. 金芬, 张忠寿, 黄卫锋. 上皮间质转化和内皮间质转化在肾纤维化中的研究进展. 海南医学, 2014, 18: 2723-2725.
8. Konoeda C, Koinuma D, Morishita Y,et al. Epithelial to mesenchymal transition in murine tracheal allotransplantation: an immunohistochemical observation. Transplant Proc, 2013, 45(5): 1797-1801.
9. Zavadil J, Bottinger EP. TGF-beta and epithelial-to-mesenchymal transitions. Oncogene, 2005, 24(37): 5764-5774.
10. Borthwick LA, Corris PA, Mahida R,et al. TNFalpha from classically activated macrophages accentuates epithelial to mesenchymal transition in obliterative bronchiolitis. Am J Transplant, 2013, 13(3): 621-633.
11. Gardner A, Fisher AJ, Richter C,et al. The critical role of TAK1 in accentuated epithelial to mesenchymal transition in obliterative bronchiolitis after lung transplantation. Am J Pathol, 2012, 180(6): 2293-2308.
12. Herrinton LJ, Harrold LR, Liu L,et al. Association between anti-TNF-alpha therapy and interstitial lung disease. Pharmacoepidemiol Drug Saf, 2013, 22(4): 394-402.
13. Roh YS, Song J, Seki E. TAK1 regulates hepatic cell survival and carcinogenesis. J Gastroenterol, 2014, 49(2): 185-194.
14. 段鸿涛, 刘红刚, 闫小龙, 等. TGF-β/Smad介导上皮细胞间质转化在气管移植后移植体狭窄中的机制研究. 中国胸心血管外科临床杂志, 2016, 23(5): 485-489.
15. Felton VM1, Inge LJ, Willis BC,et al. Immunosuppression-induced bronchial epithelial-mesenchymal transition: a potential contributor to obliterative bronchiolitis. J Thorac Cardiovasc Surg, 2011, 141(2): 523-530.
16. Grove DA, Xu J, Joodi R,et al. Attenuation of early airway obstruction by mesenchymal stem cells in a murine model of heterotopic tracheal transplantation. J Heart Lung Transplant, 2011, 30(3): 341-350.
17. Grove DA, Xu J, Joodi R,et al. TWEAK enhances TGF-beta-induced epithelial-mesenchymal transition in human bronchial epithelial cells. Respir Res, 2015, 16: 48.doi: 10.1186/s12931-015-0207-5.
18. Ji Q, Liu X, Han Z,et al. Resveratrol suppresses epithelial-to-mesenchymal transition in colorectal cancer through TGF-beta1/Smads signaling pathway mediated Snail/E-cadherin expression. BMC Cancer, 2015, 15: 97. doi: 10.1186/s12885-015-1119-y.
19. Chen L, Li W, Qiu W,et al. RSRC1 SUMOylation enhances SUMOylation and inhibits transcriptional activity of estrogen receptor beta. FEBS Lett, 2015, 589(13): 1476-1484.
20. 黄颂, 孟爱民. TGF-β调节的EMT在肿瘤浸润、转移中作用的研究进展. 生命科学, 2015, 27(2): 127-134.

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Synergy of TGF-β1 and TNF-α inducing human bronchial epithelial cells to optimize epithelia-mesenchymal transformation model

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