Cervical heart transplantation in mice model_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

CAO Jianbin ,  GONG Yongquan , WEI Chengxin , LI Fuli , LUO Peng

Department of Cardiothoracic Surgery, Liuzhou General Hospital, Liuzhou, 545003, Guangxi, P.R.China;

Corresponding author：

GONG Yongquan, Email: gongyongquan01@hotmail.com

Keywords：

Heart transplantation; animal model; mice

DOI：

10.7507/1007-4848.201609010

Video：

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Objective To investigate how to establish stable mice cervical heart transplantation model. Methods Totally, 40 male C57 mice with the age of 6-8 weeks and weight of 19-24 g were randomly divided into recipients and donors (n=20 in each group). Mice cervical heart transplantation model was established by connecting the ascending aorta of donors to the right cervical common artery of recipients through end to side anastmosis and the pulmonary artery of donors to the right external jugular vein of recipients through end to end anastmosis. Results More than 95% recipients survived after surgery. Cold ischemia time was 15±5 min, warm ischemia time 23±6 min, and the whole operation took about 55±15 min. The recipients survived more than 30 d with functional heart grafts. Histologically, there was no difference between the heart graft one month after the transplantion and the normal heart. Conclusion Cervical heart transplantation of mice model is reliable and feasible, which is easy to monitor the survival condition of heart graft by visual examination and palpation, which will benefit the basic research in transplantation field.

Citation： CAO Jianbin, GONG Yongquan, WEI Chengxin, LI Fuli, LUO Peng. Cervical heart transplantation in mice model. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2017, 24(11): 886-889. doi: 10.7507/1007-4848.201609010 Copy

1.	Corry RJ, Winn HJ, Russell PS. Primarily vascularized allografts of hearts in mice. The role of H-2D, H-2K, and non-H-2 antigens in rejection. Transplantation, 1973, 16(4):343-350.
2.	Plenter RJ, Zamora MR, Grazia TJ. Four decades of vascularized heterotopic cardiac transplantation in the mouse. J Invest Surg, 2013, 26(4):223-228.
3.	何莹, 宫念樵. 心脏移植动物模型的构建. 实用器官移植电子杂志, 2016, 4(2):88-92.
4.	郁雷, 高丽. 小鼠腹部心脏移植模型建立及并发症预防. 中国胸心血管外科临床杂志, 2014, 21(2):249-252.
5.	单忠贵, 马春野, 廖崇先, 等. 小鼠腹腔异位心脏移植模型制作技术的改进. 吉林大学学报(医学版), 2009, 35(1): 188-190.
6.	陈心足, Wang SJ, Zhou C, 等. 同种异体小鼠腹腔异位心脏移植模型的学习曲线. 中国胸心血管外科临床杂志, 2015, 22(9): 879-882.
7.	陈义发, 尹辉, 梁宾勇, 等. 两种异位心脏移植小鼠模型的建立和比较. 中国实验动物学报, 2010, 18(1):17-20.
8.	周景师, 窦科峰, 孙凯, 等. 两种小鼠异位心脏移植模型的复制及比较. 医学研究生学报, 2003, 16(9):653-655.
9.	Chen ZH. A technique of cervical heterotopic heart transplantation in mice. Transplantation, 1991, 52(6): 1099-1101.
10.	Gong W, Thornley T, Whitcher GH, et al. Introduction of modified cervical cardiac transplant model in mice. Exp Clin Transplant, 2012, 10(2):158-162.
11.	Fang J, He L, Wang SQ, et al. A simplified two-stitch sleeve technique for arterial anastomosis of cervical heterotopic cardiac transplantation in mice. Am J Transl Res, 2013, 5(5): 521-529.
12.	陈文伟, 杨亦荣, 夏鹏, 等. 小鼠颈部异位心脏移植手术的改良. 温州医学院学报, 2011, 41(3): 252-254.
13.	柳祎, 孙宗全. 小鼠颈部异位心脏移植技术的改进. 华中科技大学学报(医学版), 2003, 32(5): 522-525.
14.	Ratschiller T, Deutsch MA, Calzada-Wack J, et al. Heterotopic cervical heart transplantation in mice. J Vis Exp, 2015, (102): e52907.
15.	Oberhuber R, Cardini B, Kofler M. Murine cervical heart transplantation model using a modified cuff technique. J Vis Exp, 2014; (92): e50753.
16.	张向辉, 刘晓萍, 宋丽绅. 介绍一种改良的小鼠颈部心脏移植模型建立方法. 山东医药, 2014, 54(21): 29-32.
17.	刘琛, 王斌, 王春生. 单人袖套法建立小鼠颈部心脏移植模型. 上海医学, 2013, 36(5): 446-448.
18.	Matsuura A, Abe T, Yasuura K. Simplified mouse cervical heart transplantation using a cuff technique. Transplantation, 1991, 51(4): 896-898.
19.	高开柱, 孙宗全, 杜心灵, 等. 套管法建立小鼠颈部心脏移植模型的探讨. 华中科技大学学报(医学版), 2006, 35(5): 638-640.
20.	Niimi M. The technique for heterotopic cardiac transplantationin mice: experience of 3000 operations by one surgeon. J Heart Lung Transplant, 2001, 20(10): 1123-1128.

1. Corry RJ, Winn HJ, Russell PS. Primarily vascularized allografts of hearts in mice. The role of H-2D, H-2K, and non-H-2 antigens in rejection. Transplantation, 1973, 16(4):343-350.
2. Plenter RJ, Zamora MR, Grazia TJ. Four decades of vascularized heterotopic cardiac transplantation in the mouse. J Invest Surg, 2013, 26(4):223-228.
3. 何莹, 宫念樵. 心脏移植动物模型的构建. 实用器官移植电子杂志, 2016, 4(2):88-92.
4. 郁雷, 高丽. 小鼠腹部心脏移植模型建立及并发症预防. 中国胸心血管外科临床杂志, 2014, 21(2):249-252.
5. 单忠贵, 马春野, 廖崇先, 等. 小鼠腹腔异位心脏移植模型制作技术的改进. 吉林大学学报(医学版), 2009, 35(1): 188-190.
6. 陈心足, Wang SJ, Zhou C, 等. 同种异体小鼠腹腔异位心脏移植模型的学习曲线. 中国胸心血管外科临床杂志, 2015, 22(9): 879-882.
7. 陈义发, 尹辉, 梁宾勇, 等. 两种异位心脏移植小鼠模型的建立和比较. 中国实验动物学报, 2010, 18(1):17-20.
8. 周景师, 窦科峰, 孙凯, 等. 两种小鼠异位心脏移植模型的复制及比较. 医学研究生学报, 2003, 16(9):653-655.
9. Chen ZH. A technique of cervical heterotopic heart transplantation in mice. Transplantation, 1991, 52(6): 1099-1101.
10. Gong W, Thornley T, Whitcher GH, et al. Introduction of modified cervical cardiac transplant model in mice. Exp Clin Transplant, 2012, 10(2):158-162.
11. Fang J, He L, Wang SQ, et al. A simplified two-stitch sleeve technique for arterial anastomosis of cervical heterotopic cardiac transplantation in mice. Am J Transl Res, 2013, 5(5): 521-529.
12. 陈文伟, 杨亦荣, 夏鹏, 等. 小鼠颈部异位心脏移植手术的改良. 温州医学院学报, 2011, 41(3): 252-254.
13. 柳祎, 孙宗全. 小鼠颈部异位心脏移植技术的改进. 华中科技大学学报(医学版), 2003, 32(5): 522-525.
14. Ratschiller T, Deutsch MA, Calzada-Wack J, et al. Heterotopic cervical heart transplantation in mice. J Vis Exp, 2015, (102): e52907.
15. Oberhuber R, Cardini B, Kofler M. Murine cervical heart transplantation model using a modified cuff technique. J Vis Exp, 2014; (92): e50753.
16. 张向辉, 刘晓萍, 宋丽绅. 介绍一种改良的小鼠颈部心脏移植模型建立方法. 山东医药, 2014, 54(21): 29-32.
17. 刘琛, 王斌, 王春生. 单人袖套法建立小鼠颈部心脏移植模型. 上海医学, 2013, 36(5): 446-448.
18. Matsuura A, Abe T, Yasuura K. Simplified mouse cervical heart transplantation using a cuff technique. Transplantation, 1991, 51(4): 896-898.
19. 高开柱, 孙宗全, 杜心灵, 等. 套管法建立小鼠颈部心脏移植模型的探讨. 华中科技大学学报(医学版), 2006, 35(5): 638-640.
20. Niimi M. The technique for heterotopic cardiac transplantationin mice: experience of 3000 operations by one surgeon. J Heart Lung Transplant, 2001, 20(10): 1123-1128.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Cervical heart transplantation in mice model

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