Endotheial progenitor cell attenuates the ischemia-reperfusion injury after lung transplantation_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

WANG Muye ¹ , LIAO Chen ² , WANG Lijuan ³ , TONG Chuandi ⁴ ,  GAO Wei ¹

1. Department of Anesthesia, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, P.R.China;
2. Department of Histology and Embryology, Harbin Medical University, Harbin, 150086, P.R.China;
3. Deptarment of Anesthesia, Fuwai Hospital, National Center for Cardiovascular Diseases, Peking Union Medical College, Beijing, 100037, P.R.China;
4. Department of Anesthesia, Huashan Hospital Affiliated to Fudan University, Shanghai, 200040, P.R.China;

Corresponding author：

GAO Wei, Email: 530928515@qq.com

Keywords：

Lung transplantation; lung transplantation; endothelial progenitor cell

DOI：

10.7507/1007-4848.201709064

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To examine the effect of endothelial progenitor cell (EPC) on lung ischemia-reperfusion injury (LIRI). Methods Twenty-four recipients were randomized into 3 groups including a sham group, a LIRI group, and an EPC group. Rats in the sham group only received anesthesia. Rats in the LIRI and EPC groups received left lung transplantation and received saline or EPC immediately after reperfusion. The partial pressure of oxygen to fraction of inspiratory oxygen (PaO₂/FiO₂) ratio, wet-to-dry weight ratio and protein levels in the transplanted lung and inflammation-related factors levels in serum were examined. Histological change of transplanted lung were analyzed. The nuclear factor (NF)-κB in the transplanted lung was detected. Results Compared with the LIRI group, the PaO₂/FiO₂ ratio dramaticly increased, and the wet-to-dry weight ratio and protein level significantly decreased by EPC after reperfusion. The lung histological injury was attenuated by EPC. The pro-inflammatory factors in serum were down-regulated, whereas IL-10 was up-regulated in the EPC group. The expression of NF-κB was decreased by EPC. Conclusion EPC ameliorated LIRI after lung transplantation. The protection of EPC partly associated with anti-inflammation.

Citation： WANG Muye, LIAO Chen, WANG Lijuan, TONG Chuandi, GAO Wei. Endotheial progenitor cell attenuates the ischemia-reperfusion injury after lung transplantation. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2018, 25(7): 610-615. doi: 10.7507/1007-4848.201709064 Copy

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2.	Fu SL, Pang H, Xu JZ, et al. C/EBPβ mediates osteoclast recruitment by regulating endothelial progenitor cell expression of SDF-1α. PLoS One, 2014, 9(3): e91217.
3.	Lam CF, Liu YC, Hsu JK, et al. Autologous transplantation of endothelial progenitor cells attenuates acute lung injury in rabbits. Anesthesiology, 2008, 108(3): 392-401.
4.	Takahashi M, Matsuoka Y, Sumide K, et al. CD133 is a positive marker for a distinct class of primitive human cord blood-derived CD34-negative hematopoietic stem cells. Leukemia, 2014, 28(6): 1308-1315.
5.	Gao W, Liu D, Li D, et al. Effects of hypercapnia on T cells in lung ischemia/reperfusion injury after lung transplantation. Exp Biol Med (Maywood), 2014, 239(12): 1597-1605.
6.	高伟, 陈小杰, 宋春雨, 等. CD4+T 淋巴细胞在肺移植缺血再灌注损伤中的作用. 哈尔滨医科大学学报, 2010, 44(2): 99-102, 封底.
7.	崔晓光, 郭悦平, 高伟, 等. 治疗性高碳酸血症对大鼠移植肺细胞凋亡的影响. 中华麻醉学杂志, 2007, 27(10): 918-920.
8.	Ng CS, Wan S, Arifi AA, et al. Inflammatory response to pulmonary ischemia-reperfusion injury. Surg Today, 2006, 36(3): 205-214.
9.	Deanfield JE, Halcox JP, Rabelink TJ. Endothelial function and dysfunction: testing and clinical relevance. Circulation, 2007, 115(10): 1285-1295.
10.	Whitson BA, Prekker ME, Herrington CS, et al. Primary graft dysfunction and long-term pulmonary function after lung transplantation. J Heart Lung Transplant, 2007, 26(10): 1004-1011.
11.	de Perrot M, Liu M, Waddell TK, et al. Ischemia-reperfusion-induced lung injury. Am J Respir Crit Care Med, 2003, 167(4): 490-511.
12.	Goto T, Ishizaka A, Kobayashi F, et al. Importance of tumor necrosis factor-alpha cleavage process in post-transplantation lung injury in rats. Am J Respir Crit Care Med, 2004, 170(11): 1239-1246.
13.	Shaw MJ, Shennib H, Bousette N, et al. Effect of endothelin receptor antagonist on lung allograft apoptosis and NOSII expression. Ann Thorac Surg, 2001, 72(2): 386-390.
14.	Ross SD, Kron IL, Gangemi JJ, et al. Attenuation of lung reperfusion injury after transplantation using an inhibitor of nuclear factor-kappaB. Am J Physiol Lung Cell Mol Physiol, 2000, 279(3): L528-L536.
15.	Qiu J, Li W, Feng S, et al. Transplantation of bone marrow-derived endothelial progenitor cells attenuates cerebral ischemia and reperfusion injury by inhibiting neuronal apoptosis, oxidative stress and nuclear factor-κB expression. Int J Mol Med, 2013, 31(1): 91-98.
16.	Opal SM, De Palo VA. Anti-inflammatory cytokines. Chest, 2000, 117(4): 1162-1172.

1. 谭科芳, 孙璇. 内皮祖细胞移植治疗的研究进展和临床应用前景. 中南大学学报(医学版), 2014, 39(11): 1211-1216.
2. Fu SL, Pang H, Xu JZ, et al. C/EBPβ mediates osteoclast recruitment by regulating endothelial progenitor cell expression of SDF-1α. PLoS One, 2014, 9(3): e91217.
3. Lam CF, Liu YC, Hsu JK, et al. Autologous transplantation of endothelial progenitor cells attenuates acute lung injury in rabbits. Anesthesiology, 2008, 108(3): 392-401.
4. Takahashi M, Matsuoka Y, Sumide K, et al. CD133 is a positive marker for a distinct class of primitive human cord blood-derived CD34-negative hematopoietic stem cells. Leukemia, 2014, 28(6): 1308-1315.
5. Gao W, Liu D, Li D, et al. Effects of hypercapnia on T cells in lung ischemia/reperfusion injury after lung transplantation. Exp Biol Med (Maywood), 2014, 239(12): 1597-1605.
6. 高伟, 陈小杰, 宋春雨, 等. CD4+T 淋巴细胞在肺移植缺血再灌注损伤中的作用. 哈尔滨医科大学学报, 2010, 44(2): 99-102, 封底.
7. 崔晓光, 郭悦平, 高伟, 等. 治疗性高碳酸血症对大鼠移植肺细胞凋亡的影响. 中华麻醉学杂志, 2007, 27(10): 918-920.
8. Ng CS, Wan S, Arifi AA, et al. Inflammatory response to pulmonary ischemia-reperfusion injury. Surg Today, 2006, 36(3): 205-214.
9. Deanfield JE, Halcox JP, Rabelink TJ. Endothelial function and dysfunction: testing and clinical relevance. Circulation, 2007, 115(10): 1285-1295.
10. Whitson BA, Prekker ME, Herrington CS, et al. Primary graft dysfunction and long-term pulmonary function after lung transplantation. J Heart Lung Transplant, 2007, 26(10): 1004-1011.
11. de Perrot M, Liu M, Waddell TK, et al. Ischemia-reperfusion-induced lung injury. Am J Respir Crit Care Med, 2003, 167(4): 490-511.
12. Goto T, Ishizaka A, Kobayashi F, et al. Importance of tumor necrosis factor-alpha cleavage process in post-transplantation lung injury in rats. Am J Respir Crit Care Med, 2004, 170(11): 1239-1246.
13. Shaw MJ, Shennib H, Bousette N, et al. Effect of endothelin receptor antagonist on lung allograft apoptosis and NOSII expression. Ann Thorac Surg, 2001, 72(2): 386-390.
14. Ross SD, Kron IL, Gangemi JJ, et al. Attenuation of lung reperfusion injury after transplantation using an inhibitor of nuclear factor-kappaB. Am J Physiol Lung Cell Mol Physiol, 2000, 279(3): L528-L536.
15. Qiu J, Li W, Feng S, et al. Transplantation of bone marrow-derived endothelial progenitor cells attenuates cerebral ischemia and reperfusion injury by inhibiting neuronal apoptosis, oxidative stress and nuclear factor-κB expression. Int J Mol Med, 2013, 31(1): 91-98.
16. Opal SM, De Palo VA. Anti-inflammatory cytokines. Chest, 2000, 117(4): 1162-1172.

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Endotheial progenitor cell attenuates the ischemia-reperfusion injury after lung transplantation

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