Effect of ozone on oxidative stress and energy metabolism change of blood from aortic dissection patients <i>in vitro</i>_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

DENG Li , YANG Hui , LIU Hongyu , SU Jingjing , HU Guang ,  CHI Chao

Department of Cardiovascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, 150006, P.R.China;

Corresponding author：

CHI Chao, Email: chichao0000@163.com

Keywords：

Aortic dissection; ozone; oxidative stress; energy metabolism

DOI：

10.7507/1007-4848.201808008

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effect of ozone on oxidative stress and energy metabolism change of blood from aortic dissection (AD) patients for providing preliminary evidence of application of ozonated autohemotherapy (ozone-AHT) in AD patients. Methods Twenty AD patients (16 males and 4 females with a mean age of 48.51±10.21 years) were consecutively included in the First Affiliated Hospital of Harbin Medical University from March 2016 to August 2016, and blood samples were collected from all participants and ozonized in vitro at different ozone concentrations (0 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml, 160 μg/ml). Malondialdehyde (MDA), red blood cells (RBCs) superoxide dismutase (SOD), Na⁺-K⁺-ATP, 2,3-bisphosphoglyceric acid (2,3-DPG) at different ozone concentrations were evaluated by enzyme-linked immunosorbent assay (ELISA). Results In the control group (0 μg/ml), the content of postoperative MDA was significantly higher than that of preoperation (P<0.05). The contents of postoperative SOD, Na⁺-K⁺-ATP and 2,3-DPG were significantly lower than that of preoperation (P<0.05). The content of MDA at the concentrations of 40 μg/ml, 60 μg/ml, 80 μg/ml group increased after the operation (P>0.05), and the SOD, Na⁺-K⁺-ATP, 2,3-DPG decreased compared with the preoperation (P>0.05). But all the values were not statistically significant at the concentrations of 40 μg/ml, 80 μg/ml and 160 μg/ml respectively between preoperation and postoperation (P>0.05). Compared with other concentration groups, the content of preoperative and postoperative MDA increased in the ozone group (160 μg/ml), and oppositely, the contents ofpreoperative and postoperative SOD, Na⁺-K⁺- ATP and 2,3-DPG decreased (P<0.05). Conclusion The concentrations of 40 to 80 μg/ml of ozone can improve the antioxidant capacity of erythrocyte membrane, reduce oxidative stress in blood samples of AD patients and improve the energy metabolism of erythrocyte membranes, so the concentration range of ozone is safe and feasible for the ozone-AHT of perioperative AD.

Citation： DENG Li, YANG Hui, LIU Hongyu, SU Jingjing, HU Guang, CHI Chao. Effect of ozone on oxidative stress and energy metabolism change of blood from aortic dissection patients in vitro. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2019, 26(5): 465-469. doi: 10.7507/1007-4848.201808008 Copy

1.	Liu N, Zhang W, Ma W, et al. Risk factors for hypoxemia following surgical repair of acute type A aortic dissection. Interact Cardiovasc Thorac Surg, 2017, 24(2): 251-256.
2.	Ge H, Jiang Y, Jin Q, et al. Nomogram for the prediction of postoperative hypoxemia in patients with acute aortic dissection. BMC Anesthesiol, 2018, 18(1): 146.
3.	Bocci V, Borrelli E, Travagli V, et al. The ozone paradox: ozone is a strong oxidant as well as a medical drug. Med Res Rev, 2009, 29(4): 646-682.
4.	Bocci V, Zanardi I, Travagli V, et al. Oxygenation-ozonation of blood during extracorporeal circulation: in vitro efficiency of a new gas exchange device. Artif Organs, 2007, 31(9): 743-748.
5.	Re L, Rowen R, Travagli V. Ozone therapy and its use in medicine: further comments. Cardiology, 2017, 136(4): 269.
6.	Üreyen ÇM, Baş CY, Arslan Ş. Myocardial infarction after ozone therapy: is ozone Therapy Dr. Jekyll or Mr. Hyde? Cardiology, 2017, 137(1): 20-21.
7.	Molinari F, Simonetti V, Franzini M, et al. Ozone autohemotherapy induces long-term cerebral metabolic changes in multiple sclerosis patients. Int J Immunopathol Pharmacol, 2014, 27(3): 379-389.
8.	Sheng W, Yang HQ, Chi YF, et al. Independent risk factors for hypoxemia after surgery for acute aortic dissection. Saudi Med J, 2015, 36(8): 940-946.
9.	Liu Z, Pang X, Zhang X, et al. Incidence and risk factors of delirium in patients after type-a aortic dissection surgery. J Cardiothorac Vasc Anesth, 2017, 31(6): 1996-1999.
10.	McDonald CI, Fraser JF, Coombes JS, et al. Oxidative stress during extracorporeal circulation. Eur J Cardiothorac Surg, 2014, 46(6): 937-943.
11.	Melek FE, Baroncini LA, Repka JC, et al. Oxidative stress and inflammatory response increase during coronary artery bypass grafting with extracorporeal circulation. Rev Bras Cir Cardiovasc, 2012, 27(1): 61-65.
12.	Test ST, Mitsuyoshi J. Activation of the alternative pathway of complement by calcium-loaded erythrocytes resulting from loss of membrane phospholipid asymmetry. J Lab Clin Med, 1997, 130(2): 169-182.
13.	Mosior M, Mikołajczak A, Gomułkiewicz J. The effect of ATP on the order and the mobility of lipids in the bovine erythrocyte membrane. Biochim Biophys Acta, 1990, 1022(3): 361-364.
14.	García-de-la-Asunción J, Pastor E, Perez-Griera J, et al. Oxidative stress injury after on-pump cardiac surgery: effects of aortic cross clamp time and type of surgery. Redox Rep, 2013, 18(5): 193-199.
15.	Soto ME, Zuñiga-Muñoz A, Guarner Lans V, et al. Infusion of Hibiscus sabdariffa L. Modulates oxidative stress in patients with Marfan syndrome. Mediators Inflamm, 2016, 2016: 8625203.
16.	Billings FT 4th, Ball SK, Roberts LJ 2nd, et al. Postoperative acute kidney injury is associated with hemoglobinemia and an enhanced oxidative stress response. Free Radic Biol Med, 2011, 50(11): 1480-1487.
17.	Almaz ME, Sönmez IŞ. Ozone therapy in the management and prevention of caries. J Formos Med Assoc, 2015, 114(1): 3-11.
18.	Smith NL, Wilson AL, Gandhi J, et al. Ozone therapy: an overview of pharmacodynamics, current research, and clinical utility. Med Gas Res, 2017, 7(3): 212-219.
19.	Wang L, Chen Z, Liu Y, et al. Ozone oxidative postconditioning inhibits oxidative stress and apoptosis in renal ischemia and reperfusion injury through inhibition of MAPK signaling pathway. Drug Des Devel Ther, 2018, 12: 1293-1301.
20.	Wasser G. Ischemic stroke penumbra and extracorporeal ozone treatment. Neuroradiol J, 2013, 26(3): 243-251.

1. Liu N, Zhang W, Ma W, et al. Risk factors for hypoxemia following surgical repair of acute type A aortic dissection. Interact Cardiovasc Thorac Surg, 2017, 24(2): 251-256.
2. Ge H, Jiang Y, Jin Q, et al. Nomogram for the prediction of postoperative hypoxemia in patients with acute aortic dissection. BMC Anesthesiol, 2018, 18(1): 146.
3. Bocci V, Borrelli E, Travagli V, et al. The ozone paradox: ozone is a strong oxidant as well as a medical drug. Med Res Rev, 2009, 29(4): 646-682.
4. Bocci V, Zanardi I, Travagli V, et al. Oxygenation-ozonation of blood during extracorporeal circulation: in vitro efficiency of a new gas exchange device. Artif Organs, 2007, 31(9): 743-748.
5. Re L, Rowen R, Travagli V. Ozone therapy and its use in medicine: further comments. Cardiology, 2017, 136(4): 269.
6. Üreyen ÇM, Baş CY, Arslan Ş. Myocardial infarction after ozone therapy: is ozone Therapy Dr. Jekyll or Mr. Hyde? Cardiology, 2017, 137(1): 20-21.
7. Molinari F, Simonetti V, Franzini M, et al. Ozone autohemotherapy induces long-term cerebral metabolic changes in multiple sclerosis patients. Int J Immunopathol Pharmacol, 2014, 27(3): 379-389.
8. Sheng W, Yang HQ, Chi YF, et al. Independent risk factors for hypoxemia after surgery for acute aortic dissection. Saudi Med J, 2015, 36(8): 940-946.
9. Liu Z, Pang X, Zhang X, et al. Incidence and risk factors of delirium in patients after type-a aortic dissection surgery. J Cardiothorac Vasc Anesth, 2017, 31(6): 1996-1999.
10. McDonald CI, Fraser JF, Coombes JS, et al. Oxidative stress during extracorporeal circulation. Eur J Cardiothorac Surg, 2014, 46(6): 937-943.
11. Melek FE, Baroncini LA, Repka JC, et al. Oxidative stress and inflammatory response increase during coronary artery bypass grafting with extracorporeal circulation. Rev Bras Cir Cardiovasc, 2012, 27(1): 61-65.
12. Test ST, Mitsuyoshi J. Activation of the alternative pathway of complement by calcium-loaded erythrocytes resulting from loss of membrane phospholipid asymmetry. J Lab Clin Med, 1997, 130(2): 169-182.
13. Mosior M, Mikołajczak A, Gomułkiewicz J. The effect of ATP on the order and the mobility of lipids in the bovine erythrocyte membrane. Biochim Biophys Acta, 1990, 1022(3): 361-364.
14. García-de-la-Asunción J, Pastor E, Perez-Griera J, et al. Oxidative stress injury after on-pump cardiac surgery: effects of aortic cross clamp time and type of surgery. Redox Rep, 2013, 18(5): 193-199.
15. Soto ME, Zuñiga-Muñoz A, Guarner Lans V, et al. Infusion of Hibiscus sabdariffa L. Modulates oxidative stress in patients with Marfan syndrome. Mediators Inflamm, 2016, 2016: 8625203.
16. Billings FT 4th, Ball SK, Roberts LJ 2nd, et al. Postoperative acute kidney injury is associated with hemoglobinemia and an enhanced oxidative stress response. Free Radic Biol Med, 2011, 50(11): 1480-1487.
17. Almaz ME, Sönmez IŞ. Ozone therapy in the management and prevention of caries. J Formos Med Assoc, 2015, 114(1): 3-11.
18. Smith NL, Wilson AL, Gandhi J, et al. Ozone therapy: an overview of pharmacodynamics, current research, and clinical utility. Med Gas Res, 2017, 7(3): 212-219.
19. Wang L, Chen Z, Liu Y, et al. Ozone oxidative postconditioning inhibits oxidative stress and apoptosis in renal ischemia and reperfusion injury through inhibition of MAPK signaling pathway. Drug Des Devel Ther, 2018, 12: 1293-1301.
20. Wasser G. Ischemic stroke penumbra and extracorporeal ozone treatment. Neuroradiol J, 2013, 26(3): 243-251.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Effect of ozone on oxidative stress and energy metabolism change of blood from aortic dissection patients in vitro

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content