LINC00636 regulates the proliferation and migration of non-small cell lung cancer cells by targeting NM23-H1_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

 LU Zhansheng , ZHANG Qingbin , ZHOU Xiaojiang , HU Jian , YAO Jing , TANG Weijuan

Department of Thoracic Surgery, Guizhou Provincial People's Hospital, Guiyang, 550002, P. R. China;

Corresponding author：

LU Zhansheng, Email: zbgr7671754@163.com

Keywords：

LINC00636; NM23-H1; non-small cell lung cancer; metastasis

DOI：

10.7507/1007-4848.202410087

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the mechanism by which LINC00636 promotes the proliferation and metastasis of non-small cell lung cancer (NSCLC) by targeting NM23-H1. Methods Based on the Affymetrix® GeneChip Human Transcriptome Array 2.0 (HTA2.0), differentially expressed long non-coding RNA (lncRNA) in three pairs of NSCLC tissues with and without lymph node metastasis were analyzed, and LINC00636 was selected as the candidate gene. The effects of LINC00636 knockdown and overexpression on the proliferation, apoptosis, migration and subcutaneous tumorigenicity of PC9 cells were analyzed. The dual-luciferase assay was used to analyze the targeting regulation of LINC00636 on NM23-H1. Results The expression of LINC00636 in the serum and tissues of NSCLC patients with lymph node metastasis was higher than that in patients without lymph node metastasis. LINC00636 knockdown could inhibit the invasion and proliferation of NSCLC cells. LINC00636 could target and regulate the expression of NM23-H1, and the expression of LINC00636 and NM23-H1 in NSCLC tissues and cells was negatively correlated. The inhibitory effect of NM23-H1 on the proliferation and invasion of PC9 cells could be partially reversed by LINC00636 overexpression. Conclusion LINC00636 promotes the proliferation and metastasis of NSCLC cells by inhibiting the expression of NM23-H1 and is a potential prognostic marker and therapeutic target for NSCLC.

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29.	姚菲菲, 王军, 何爱萍. LncRNA HOTAIR通过Wnt/β-Catenin信号通路调控NSCLC细胞增殖, 迁移及侵袭. 实用癌症杂志, 2021, 36(2): 7.Yao FF, Wang J, He AP. LncRNA HOTAIR regulates NSCLC cell proliferation, migration and invasion through the Wnt/β-Catenin signaling pathway. Pract J Cancer, 2021, 36(2): 7.
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31.	Fan Y, Shixu L, Siyang D, et al. Expression profile analysis of long noncoding RNA in HER-2-enriched subtype breast cancer by next-generation sequencing and bioinformatics. Oncotargets Ther, 2016, 9: 761-772.
32.	Yingcheng W, Jinhuan W, Yue M, et al. Orchestrating a biomarker panel with lncRNAs and mRNAs for predicting survival in pancreatic ductal adenocarcinoma. J Cell Biochem, 2018, 119.
33.	Steeg PS, Bevilacqua G, Sobel ME, et al. Identification and characterization of differentially expressed genes in tumor metastasis: The nm23 gene. Basic Life Sci, 1991, 57: 355-360.
34.	Yu L, Wang X, Zhang W, et al. The multiple regulation of metastasis suppressor NM23-H1 in cancer. Life Sci, 2021, 268: 118995.
35.	Li D, Li W, Tian C, et al. Prognostic and clinicopathological significance of nm23-H1 expression in non-small cell lung cancer: A meta-analysis. Medicine (Baltimore), 2022, 101(39): e30815.

1. Peng WX, Koirala P, Mo YY. LncRNA-mediated regulation of cell signaling in cancer. Oncogene, 2017, 36(41): 5661-5667.
2. Müller V, Oliveira-Ferrer L, Steinbach B, et al. Interplay of lncRNA H19/miR-675 and lncRNA NEAT1/miR-204 in breast cancer. Mol Oncol, 2019, 13(5): 1137-1149.
3. Li J, Meng H, Bai Y, et al. Regulation of lncRNA and its role in cancer metastasis. Oncol Res, 2016, 23(5): 205-217.
4. Zhang Y, Liu J, Lv Y, et al. LncRNA meg3 suppresses hepatocellular carcinoma in vitro and vivo studies. Am J Transl Res, 2019, 11(7): 4089-4099.
5. Pan W, Li W, Zhao J, et al. lncRNA-PDPK2P promotes hepatocellular carcinoma progression through the PDK1/AKT/Caspase 3 pathway. Mol Oncol, 2019, 13(10): 2246-2258.
6. Chao Y, Zhou D. lncRNA-D16366 is a potential biomarker for diagnosis and prognosis of hepatocellular carcinoma. Med Sci Monit, 2019, 25: 6581-6586.
7. Gupta M, Chandan K, Sarwat M. Role of microRNA and long non-coding RNA in hepatocellular carcinoma. Curr Pharm Des, 2020, 26(4): 415-428.
8. Suresh K, Naidoo J, Lin CT, et al. Immune checkpoint immunotherapy for non-small cell lung cancer: Benefits and pulmonary toxicities. Chest, 2018, 154(6): 1416-1423.
9. 曾丽, 张洪攀, 马代远. 放疗前诱导免疫治疗在不可切除Ⅲ期非小细胞肺癌中的研究进展. 中国肿瘤临床, 2024, 51(5): 264-268.Zeng L, Zhang HP, Ma DY. Research progress of induction immunotherapy before radiotherapy in unresectable stageⅢ non-small cell lung cancer. Chin J Clin Oncol, 2024, 51(5): 264-268.
10. Zhang J, Zhang P, Wang L, et al. Long non-coding RNA HOTAIR in carcinogenesis and metastasis. Acta Biochim Biophys Sin (Shanghai), 2014, 46(1): 1-5.
11. Huang Q, Yan J, Agami R. Long non-coding RNAs in metastasis. Cancer Metastasis Rev, 2018, 37(1): 75-81.
12. Ge X, Li GY, Jiang L, et al. Long noncoding RNA CAR10 promotes lung adenocarcinoma metastasis via miR-203/30/SNAI axis. Oncogene, 2019, 38(16): 3061-3076.
13. Herbst RS, Morgensztern D, Boshoff C. The biology and management of non-small cell lung cancer. Nature, 2018, 553(7689): 446-454.
14. 郑森中, 牟吉. 非小细胞肺癌组织ADGRD1, PTK7与淋巴结转移和预后的关系研究. 中华实验外科杂志, 2024, 41(10): 2194-2194.Zheng SZ, Mou J. Relationship between ADGRD1, PTK7 and lymph node metastasis and prognosis in non-small cell lung cancer. Chin J Exp Surg, 2024, 41(10): 2194-2194.
15. 訾瑞, 胡萍. PEG3, STMN1基因在非小细胞肺癌的表达及其与临床病理特征, 血管生成相关性研究. 中国现代医学杂志, 2024, 34(9): 70-77.Zi R, Hu P. Expression of PEG3 and STMN1 genes in non-small cell lung cancer and their correlation with clinicopathological features and angiogenesis. Chin J Mod Med, 2024, 34(9): 70-77.
16. Li J, Shi D, Li S, et al. KEAP1 promotes anti-tumor immunity by inhibiting PD-L1 expression in NSCLC. Cell Death Dis, 2024, 15(2): 175.
17. Lin H, Li J, Wang M, et al. Exosomal long noncoding RNAs in NSCLC: Dysfunctions and clinical potential. J Cancer, 2023, 14(10): 1736-1750.
18. Zhou Z, Cao Q, Diao Y, et al. Non-coding RNA-related antitumor mechanisms of marine-derived agents. Front Pharmacol, 2022, 13: 1053556.
19. Achkar NP, Cambiagno DA, Manavella PA. miRNA biogenesis: A dynamic pathway. Trends Plant Sci, 2016, 21(12): 1034-1044.
20. Yang Y, Yujiao W, Fang W, et al. The roles of miRNA, lncRNA and circRNA in the development of osteoporosis. Biol Res, 2020, 53(1): 40.
21. Wang Q, Han J, Xu P, et al. Silencing of lncRNA SNHG16 downregulates cyclin D1 (CCND1) to abrogate malignant phenotypes in oral squamous cell carcinoma (OSCC) through upregulating miR-17-5p. Cancer Manag Res, 2021, 13: 1831-1841.
22. Duan R, Li C, Wang F, et al. The long noncoding RNA ZFAS1 potentiates the development of hepatocellular carcinoma via the microRNA-624/MDK/ERK/JNK/P38 signaling pathway. Onco Targets Ther, 2020, 13: 4431-4444.
23. Fang Y, Hu J, Wang Z, et al. LncRNA H19 functions as an Aquaporin 1 competitive endogenous RNA to regulate microRNA-874 expression in LPS sepsis. Biomed Pharmacother, 2018, 105: 1183-1191.
24. Sun Z, Shi K, Yang S, et al. Effect of exosomal miRNA on cancer biology and clinical applications. Mol Cancer, 2018, 17(1): 147.
25. Guo S, Yin Y, Wang Z, et al. lncRNA TUG1 expression in NSCLC and its clinical significance. Clin Lab, 2020, 66(10): 2020.
26. Zhao Z, Wang J, Wang S, et al. LncRNA CCAT2 promotes tumorigenesis by over-expressed Pokemon in non-small cell lung cancer. Biomed Pharmacother, 2017, 87: 692-697.
27. Roh J, Kim B, Im M, et al. MALAT1-regulated gene expression profiling in lung cancer cell lines. BMC Cancer, 2023, 23(1): 818.
28. Zang C, Nie FQ, Wang Q, et al. Long non-coding RNA LINC01133 represses KLF2, P21 and E-cadherin transcription through binding with EZH2, LSD1 in non-small cell lung cancer. Oncotarget, 2016, 7(10): 11696-11707.
29. 姚菲菲, 王军, 何爱萍. LncRNA HOTAIR通过Wnt/β-Catenin信号通路调控NSCLC细胞增殖, 迁移及侵袭. 实用癌症杂志, 2021, 36(2): 7.Yao FF, Wang J, He AP. LncRNA HOTAIR regulates NSCLC cell proliferation, migration and invasion through the Wnt/β-Catenin signaling pathway. Pract J Cancer, 2021, 36(2): 7.
30. 张亚琛, 梁迪, 靳晶, 等. 非小细胞肺癌相关lncRNA的研究进展. 中国肺癌杂志, 2018, 21(1): 7.Zhang YC, Liang D, Jin J, et al. Research progress of lncRNA in non-small cell lung cancer. Chin J Lung Cancer, 2018, 21(1): 7.
31. Fan Y, Shixu L, Siyang D, et al. Expression profile analysis of long noncoding RNA in HER-2-enriched subtype breast cancer by next-generation sequencing and bioinformatics. Oncotargets Ther, 2016, 9: 761-772.
32. Yingcheng W, Jinhuan W, Yue M, et al. Orchestrating a biomarker panel with lncRNAs and mRNAs for predicting survival in pancreatic ductal adenocarcinoma. J Cell Biochem, 2018, 119.
33. Steeg PS, Bevilacqua G, Sobel ME, et al. Identification and characterization of differentially expressed genes in tumor metastasis: The nm23 gene. Basic Life Sci, 1991, 57: 355-360.
34. Yu L, Wang X, Zhang W, et al. The multiple regulation of metastasis suppressor NM23-H1 in cancer. Life Sci, 2021, 268: 118995.
35. Li D, Li W, Tian C, et al. Prognostic and clinicopathological significance of nm23-H1 expression in non-small cell lung cancer: A meta-analysis. Medicine (Baltimore), 2022, 101(39): e30815.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Latest ArticlesLINC00636 regulates the proliferation and migration of non-small cell lung cancer cells by targeting NM23-H1

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