Effect of High Mobility Group Box-1 on The Death Pathways of Pancreatic Acinar Cell in Rat with Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 WANGJun , CAIZhi-fang , LIUYao ,  DUIDan-hua

Department of General Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China;

Corresponding author：

DUIDan-hua, Email: ddh1957@163.com

Keywords：

Severe acute pancreatitis; High mobility group box-1; Oncosis; Flowcytometry

DOI：

10.7507/1007-9424.20140071

Video：

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Objective To explore the effect of the serum high mobility group box-1 (HMGB1) on oncosis of pancreatic acinar cells in the rat with severe acute pancreatitis (SAP). Methods Thirty-two healthy SD rats were randomly divided into 2 groups:sham operation group (SO group, n=8) and SAP group (n=24). Rats of SO group were only flipped the intestinal canal after laparotomy, but rats of SAP group were induced by retrograde injection of 3% sodium taurocholate into bilio-pancreatic duct in addition. Rats of SO group were sacrificed at 6 hours after operation, and rats of SAP group were sacrificed at 6 (SAP-6 hour group, n=8), 12 (SAP-12 hour group, n=8), and 24 hours (SAP-24 hour group, n=8) after operation respectively. Pancreatic tissues were stained by HE to observe pathological changes. Serum HMGB1 was measured by ELISA, and the oncosis percentage of pancreatic acinar cells was examined by flowcytometry. Results Pathological results showed that structural integrity was observed in pancreatic acinar, and occasionally a single inflammatory cell infiltration was observed in rats of SO group. Swelling, interstitial edema, and inflammatory cell infiltration were observed in rats of SAP-6 hour group. Some necrosis of pancreatic acinar cell, stromal vascular congestion, and focal necrosis were observed in rats of SAP-12 hour group and SAP-24 hour group, which the pathological damage were worse over time. Levels of serum HMGB1 and oncosis percentages of pancreatic acinar cells in rats of 3 SAP subgroups were all higher than those of SO group (P < 0.01), and the 2 kinds of indexes both increased over time (P < 0.05). There was positive correlation between concentration of serum HMGB1 and oncosis percentages of pancreatic acinar cells in SAP rat during 24 hours after operation (r=0.846, P < 0.01). Conclusions HMGB1 seems to play an important role in SAP by inducing oncosis of pancreatic acinar cells when inducing inflammatory reaction in rat with SAP.

Citation： WANGJun, CAIZhi-fang, LIUYao, DUIDan-hua. Effect of High Mobility Group Box-1 on The Death Pathways of Pancreatic Acinar Cell in Rat with Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(3): 295-299. doi: 10.7507/1007-9424.20140071 Copy

1.	Hyvönen MT, Herzig KH, Sinervirta R, et al. Activated polya-mine catabolism in acute pancreatitis:alpha-methylated polyamine analogues prevent trypsinogen activation and pancreatitis-associated mortality[J]. Am J Pathol, 2006, 168(1):115-122.
2.	王兴鹏.重视肠道衰竭在重症急性胰腺炎发病中的作用[J].中华消化杂志, 2002, 22(1):5-6.
3.	李磊, 王兴鹏.炎症反应的晚期介质HMGB1[J].国外医学:生理、病理科学与临床分册, 2004, 24(6):567-568.
4.	刘旺华, 李花, 肖献忠.高迁移率族蛋白B-1与炎症关系的研究进展[J].中国病理生理杂志, 2008, 24(8):1656-1660.
5.	Gdynia G, Keith M, Kopitz J, et al. Danger signaling protein HMGB1 induces a distinct form of cell death accompanied by formation of giant mitochondria[J]. Cancer Res, 2010, 70(21):8558-8568.
6.	郎洁, 张培建.细胞凋亡在急性胰腺炎发病机制中所起作用的研究进展[J].中国普通外科杂志, 2008, 17(9):914-916.
7.	任成山, 钱桂生.细胞凋亡和胀亡及其与多器官功能障碍综合征关系的研究进展[J].中国危重病急救医学, 2005, 17(4):247-250.
8.	楚江涛, 薛东波.急性胰腺炎腺泡细胞的死亡方式[J].中国现代普通外科进展, 2012, 15(4):302-304.
9.	Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980, 15(4):411-416.
10.	王连才, 马清涌, 陈香丽.一种改进的简便经济的大鼠胰腺腺泡细胞分离提纯方法[J].第四军医大学学报, 2008, 29(3):220-222.
11.	司徒镇强, 吴军正.细胞培养[M].西安:世界图书出版西安公司, 2007:58-59.
12.	Majno G, Joris I. Apoptosis, oncosis, and necrosis. An overview of cell death[J]. Am J Pathol, 1995, 146(1):3-15.
13.	Trump BF, Berezesky IK, Chang SH, et al. The pathways of cell death:oncosis, apoptosis, and necrosis[J]. Toxicol Pathol, 1997, 25(1):82-88.
14.	Shinoura N, Yoshida Y, Nishimura M, et al. Expression level of Bcl-2 determines anti-or proapoptotic function[J]. Cancer Res, 1999, 59(16):4119-4128.
15.	Alvarez P, Alvarado C, Puerto M, et al. Improvement of leukocyte functions in prematurely aging mice after five weeks of diet supplementation with polyphenol-rich cereals[J]. Nutrition, 2006, 22(9):913-921.
16.	Bhatia M. Apoptosis versus necrosis in acute pancreatitis[J]. Am J Physiol Gastrointest Liver Physiol, 2004, 286(2):G189-G196.
17.	Stolte M, Haas L, Wamwayi HM, et al. Induction of apoptotic cellular death in lymphatic tissues of cattle experimentally infected with different strains of rinderpest virus[J]. J Comp Pathol, 2002, 127(1):14-21.
18.	尚东, 关凤林, 杨佩满, 等.胰腺腺泡细胞凋亡在大鼠急性胰腺炎病程中的作用及凋亡调控基因的表达[J].肝胆胰外科杂志, 2001, 13(3):152-155.
19.	Ziangirova GG, Antonova OV. The causes of necrobiosis and apoptosis of corneal epithelial cells during primary acquired keratoconus[J]. Izv Akad Nauk Ser Biol, 2002, 16(5):517-521.
20.	沈美琴, 夏敏.晚期炎症因子介质高迁移率族蛋白1与重症急性胰腺炎的关系的研究进展[J].胰腺病学杂志, 2009, 19(6):371-373.
21.	Scaffidi P, Misteli T, Bianchi ME. Release of chromatin protein HMGB1 by necrotic cells triggers inflammation[J]. Nature, 2002, 418(6894):191-195.
22.	朱峰, 龙锦, 何忠野, 等.急性坏死性胰腺炎大鼠胰腺高迁移率族蛋白-1的表达及意义[J].中国普通外科杂志, 2007, 16(5):418-421.
23.	戴振华, 王亮, 孙伟, 等. HMGB1在鉴别过氧化氢诱导心肌细胞凋亡和死亡中作用的研究[J].南京医科大学学报:自然科学版, 2007, 27(9):956-959.
24.	杨智勇, 王春友, 熊炯炘, 等.重症急性胰腺炎大鼠血清高迁移率族蛋白-1水平的时相变化及意义[J].华中科技大学学报:医学版, 2004, 33(4):466-468.

1. Hyvönen MT, Herzig KH, Sinervirta R, et al. Activated polya-mine catabolism in acute pancreatitis:alpha-methylated polyamine analogues prevent trypsinogen activation and pancreatitis-associated mortality[J]. Am J Pathol, 2006, 168(1):115-122.
2. 王兴鹏.重视肠道衰竭在重症急性胰腺炎发病中的作用[J].中华消化杂志, 2002, 22(1):5-6.
3. 李磊, 王兴鹏.炎症反应的晚期介质HMGB1[J].国外医学:生理、病理科学与临床分册, 2004, 24(6):567-568.
4. 刘旺华, 李花, 肖献忠.高迁移率族蛋白B-1与炎症关系的研究进展[J].中国病理生理杂志, 2008, 24(8):1656-1660.
5. Gdynia G, Keith M, Kopitz J, et al. Danger signaling protein HMGB1 induces a distinct form of cell death accompanied by formation of giant mitochondria[J]. Cancer Res, 2010, 70(21):8558-8568.
6. 郎洁, 张培建.细胞凋亡在急性胰腺炎发病机制中所起作用的研究进展[J].中国普通外科杂志, 2008, 17(9):914-916.
7. 任成山, 钱桂生.细胞凋亡和胀亡及其与多器官功能障碍综合征关系的研究进展[J].中国危重病急救医学, 2005, 17(4):247-250.
8. 楚江涛, 薛东波.急性胰腺炎腺泡细胞的死亡方式[J].中国现代普通外科进展, 2012, 15(4):302-304.
9. Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980, 15(4):411-416.
10. 王连才, 马清涌, 陈香丽.一种改进的简便经济的大鼠胰腺腺泡细胞分离提纯方法[J].第四军医大学学报, 2008, 29(3):220-222.
11. 司徒镇强, 吴军正.细胞培养[M].西安:世界图书出版西安公司, 2007:58-59.
12. Majno G, Joris I. Apoptosis, oncosis, and necrosis. An overview of cell death[J]. Am J Pathol, 1995, 146(1):3-15.
13. Trump BF, Berezesky IK, Chang SH, et al. The pathways of cell death:oncosis, apoptosis, and necrosis[J]. Toxicol Pathol, 1997, 25(1):82-88.
14. Shinoura N, Yoshida Y, Nishimura M, et al. Expression level of Bcl-2 determines anti-or proapoptotic function[J]. Cancer Res, 1999, 59(16):4119-4128.
15. Alvarez P, Alvarado C, Puerto M, et al. Improvement of leukocyte functions in prematurely aging mice after five weeks of diet supplementation with polyphenol-rich cereals[J]. Nutrition, 2006, 22(9):913-921.
16. Bhatia M. Apoptosis versus necrosis in acute pancreatitis[J]. Am J Physiol Gastrointest Liver Physiol, 2004, 286(2):G189-G196.
17. Stolte M, Haas L, Wamwayi HM, et al. Induction of apoptotic cellular death in lymphatic tissues of cattle experimentally infected with different strains of rinderpest virus[J]. J Comp Pathol, 2002, 127(1):14-21.
18. 尚东, 关凤林, 杨佩满, 等.胰腺腺泡细胞凋亡在大鼠急性胰腺炎病程中的作用及凋亡调控基因的表达[J].肝胆胰外科杂志, 2001, 13(3):152-155.
19. Ziangirova GG, Antonova OV. The causes of necrobiosis and apoptosis of corneal epithelial cells during primary acquired keratoconus[J]. Izv Akad Nauk Ser Biol, 2002, 16(5):517-521.
20. 沈美琴, 夏敏.晚期炎症因子介质高迁移率族蛋白1与重症急性胰腺炎的关系的研究进展[J].胰腺病学杂志, 2009, 19(6):371-373.
21. Scaffidi P, Misteli T, Bianchi ME. Release of chromatin protein HMGB1 by necrotic cells triggers inflammation[J]. Nature, 2002, 418(6894):191-195.
22. 朱峰, 龙锦, 何忠野, 等.急性坏死性胰腺炎大鼠胰腺高迁移率族蛋白-1的表达及意义[J].中国普通外科杂志, 2007, 16(5):418-421.
23. 戴振华, 王亮, 孙伟, 等. HMGB1在鉴别过氧化氢诱导心肌细胞凋亡和死亡中作用的研究[J].南京医科大学学报:自然科学版, 2007, 27(9):956-959.
24. 杨智勇, 王春友, 熊炯炘, 等.重症急性胰腺炎大鼠血清高迁移率族蛋白-1水平的时相变化及意义[J].华中科技大学学报:医学版, 2004, 33(4):466-468.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of High Mobility Group Box-1 on The Death Pathways of Pancreatic Acinar Cell in Rat with Severe Acute Pancreatitis

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